Correlation Between Skin Prick Test and Enzyme-linked Immunosorbent Assay Using Serum for Identification of Subjects Positive to Major Indoor Respiratory Allergens

실내환경 주요 호흡기 알레르기 항원 감작검사를 위한 피부단자 시험과 혈청 ELISA시험의 상관성에 대한 연구

  • Published : 2008.10.31


The indoor environment is an important source of exposure to various aeroallergens and pathogenic microorganism. It has been shown that exposure to aeroallergens enhances the risk of indoor inhabitants developing asthma. Since the skin prick test, a typical clinical method for identification of subjects positive to allergens, can rarely cause fatal or non-fatal reactions in susceptible persons, an in vitro assay such as ELISA using serum has been considered for testing positivity against various allergens. We evaluated the validity of a serum ELISA kit for screening positive subjects to major aeroallergens including Dermatophagoides farinae, Dermatophagoides pteronyssinus, cockroach, Alternaria, Cladosporium, Aspergillus, Penicillium, dog hair, cat fur, mugwort, and ragweed. The ELISA results were compared with the skin prick test results, and sensitivity, specificity, and overall accuracy were calculated to each allergen. Higher sensitivities were obtained from D. farinae, (77.8%) and D. pteronyssinus (69.2%), but sensitivities to Aspergillus, Penicillium, dog hair, cat fur, and ragweed were very low down to 0%. Specificity ranged from 88.7% (cat fur) to 100% (mugwort and ragweed). Overall the accuracy of the serum ELISA kit was relatively high, in that the lowest was 85.1% for cat fur and the highest was 98.6% for Alternaria, Cladosporium, and ragweed. Considering specificity and overall accuracy for the serum ELISA kit, it may be considered reliable. However, when the kit is used for screening purpose, positivity to aeroallergens should be carefully determined since sensitivity for the kit is low.


indoor respiratory allergen;skin prick test;ELISA;validity


  1. Park, H-S., Heo, Y-J., Byun, J-A. and Heo, Y. : Immunotoxicological evaluation of pollen intake using mice model. J. Env. Health Sci., 31(4), 287-293, 2005
  2. Kim, M-K. and Oh, S-W. : Change of causative inhalant allergens in respiratory allergic patients in Chungbuk district. Korean J. Asthma Allergy Clin. Immunol., 19(5), 696-702, 1999
  3. Principles of epidemiology: Jung Soon Kim. Sin Kwang Publishing, 116-124, 2000
  4. Kim, K-H., Kim, K-T., Lee, S-K., Park, H-S., Lee YM., Nahm, D-H., Son, C-H., Yang, D-K., Roh, M-S., Choi, P-J., Lee,J-H., Kim K-N. and Lee K-N. : Sensitization rates for inhalant allergens in patients with respiratory allergy in Busan. Korean J. Asthma Allergy Clin. Immunol., 25(1), 59-63, 2005
  5. Yu, S-D., Yoo, S-E., Lee, M-J., Choi, W-H., Kim DS., Lee, C-H. and Park, Y-J.: Pulmonary function and its influence factors of elementary school children in Gangneung. J. Env. Health Sci., 34(1), 20-26, 2008
  6. Kim, S-H., Lee, J-Y., Son, S-W., Chang, Y-S., Jung J-W., Kim, Y-K., Cho, S-H., Min, K-U. and Kim, YY. : P revalence o f adult asthma b ased o n question - aires and methacholine bronchial provocation test in Seoul. Korean J. Asthma Allergy Clin. Immunol., 21(4), 618-627, 2001
  7. Park, J-B., Kim, K-Y., Jang G-Y., Kim C-N. and Lee, K-J. : Size distribution and concentration of airborne fungi in the public facilities. J. Env. Health Sci., 32(1), 36-45, 2006
  8. Simons, F.E.R., Frew, A.J., Ansotegui, I.J., Bochner, B.S., Golden, D.B.K., Finkelman, F.D., Leung, D.Y.M., Lotvall, J., Marone, G., Metcalfe, D.D., Muller, U., Rosenwasser, L.J., Sampson, H.A., Schwartz, L.B., van Hage, M. and Walls, A.F. : Risk assessment in anaphylaxis: current and future approaches. J. Allergy Clin. Immunol., 120, S2-S24, 2007
  9. Richardson, G., Eick, S. and Jones, R. : How is the indoor environment related to asthma?: literature review. J. Adv. Nursing, 52, 328-339, 2005
  10. Aas, K. and Belin, L. : Standardization of diagnostic work i n allergy. Acta Allergol 27, 439-444, 1972
  11. Blanco, C., Carrillo, T., Ortega, N., Alvarez, M., Dominguez, C. and Castillo, R. : Comparison of skin prick test and specific serum IgE determination for the diagnosis of latex allergy. Clin. Exp. Allergy, 28, 971-976, 1998
  12. Park, H-W., Kim, S-H., Kim S-H., Chang, Y-H., Chung, H., Lee, B-J., Kim, Y-K., Cho, S-H., Min, KU. and Kim, Y-Y. : Changes of atopy prevalence among asthmatics visiting tertiary referral hospital (1983, 1990, 1997). Korean J. Asthma Allergy Clin. Immunol., 20(1), 99-105, 2000
  13. D'Amato, G. : urban air pollution and plant-derived respiratory allergy. Clin. Exp. Allergy, 30, 628-636, 2000
  14. Berstein, D.I., Wanner, M., Borish, L. and Liss, G.M.: Twelve-year survey of fatal reactions to allergen injections and skin testing: 1999-2001. J. Allergy Clin. Immunol., 113, 1129-1136, 2004
  15. Roberts, G. and Lack, G. : Diagnosing peanut allergy with skin prick test and specific IgE testing. J. Allergy Clin. Immunol., 115, 1291-1296, 2005

Cited by

  1. Anti-inflammatory Effect of Cirsium Setidens Ethanol Extract on Macrophages and Effect on Immunoglobulins in Normal or Ovalbumin Sensitized Balb/c Mouse vol.26, pp.6, 2017,