Expression of laccase in transgenic tobacco chloroplasts

엽록체형질전환을 이용한 담배에서의 laccase 유전자의 발현

  • 유병호 (유진텍㈜ 부설연구소) ;
  • 임종민 (유진텍㈜ 부설연구소) ;
  • 우제욱 (유진텍㈜ 부설연구소) ;
  • 최동욱 (전남대학교 사범대학 과학교육학부 생물교육) ;
  • 김선하 (충남대학교 농업생명과학대학 응용생물학) ;
  • 최관삼 (충남대학교 농업생명과학대학 응용생물학) ;
  • 유장렬 (한국생명공학연구원 식물유전체연구센터) ;
  • 고석민 (유진텍㈜ 부설연구소)
  • Published : 2008.03.31


Laccase (EC is a small group of enzymes that catalyze the oxidation of a broad range of phenolic compounds including hazardous and recalcitrant pollutants in the environment. This study attempted to develop an efficient system for production of a recombinant laccase by chloroplast genetic transformation of tobacco. Chloroplast transformation vector was constructed and introduced into the tobacco chloroplast genome using particle bombardment. Chloroplast-transformed plants were subsequently regenerated. PCR and southern blot analyses confirmed stable integration of the laccase gene into the chloroplast genome. Northern blot analysis revealed that mRNA of the laccase gene was highly expressed in chloroplast-transformed plants.



  1. Alfred MM, Richard CS (2002) Laccase: new functions for an old enzyme. Phytochemistry 60: 551-565
  2. Alicia FM, Angel M, Michael M, Henry D (2003) A Chloroplast transgenic approach to hyper-express and purify Human Serum Albumin, a protein highly susceptible to proteolytic degradation. Plant Biotech J 1: 71-79
  3. Daniell H, Datta R, Varma S, Gray S, Lee SB (1998) Containment of herbicide resistance through genetic engineering of the chloroplast genome. Nature Biotechnol 16: 345-348
  4. Daniell H, Lee SB, Panchal T, Wiebe PO (2001) Expression of cholera toxin B subunit gene and assembly as functional oligomers in transgenic tobacco chloroplasts. J Mol Biol 311: 1001-1009
  5. Day CD, Lee E, Kobayashi J, Holappa LD, Albert H, Ow DW (2000) Transgene integration into the same chromosome location can produce alleles that express at a predictable level, or alleles that are differentially silenced. Genes Dev 14: 2869-2880
  6. De Cosa B, Moar W, Lee SB, Miller M, Daniell H. (2001) Overexpression of the Bt Cry2Aa2 operon in chloroplasts leads to formation of insecticidal crystals. Nature Biotechnol 19: 71-74
  7. Glynis G, Gordon A, Douglas B, Adrian C (2000) Transgenic plants as factories for biopharmaceuticals. Natature Biotechnol 18: 1151-1155
  8. Higuchi T (1990) Lignin biochemistry: biosynthesis and biodegradation. Wood Sci Technol 24: 23-63
  9. Hood EE, Bailey MR, Beifuss K, Magallanes-Lundback M, Horn ME, Callaway E, Drees C, Delaney DE, Clough R, Howard JA (2003) Criteria for high-level expression of a fungal laccase gene in transgenic maize. Plant Biotechnol J 1: 129-140
  10. Hood EE, Witcher DR, Maddock S, Meyer T, Baszczynski C, Mailey MR, Flynn P, Register J, Marshall L, Bond D, Kulisek E, Kusnadi A, Evangelista R, Nikolov Z, Wooge C, Mehigh RJ, Hernan R, Kappel WK, Ritland D, Li CP, Howard JA (1997) Commercial production of avidin from transgenic maize: Characterization of transformant, production, processing, extraction and purification. Mol Breed 3: 291-306
  11. Kirk TK, Farrell RL (1987) Enzymatic 'combustion': the microbial degradation of lignin. Annu. Rev Microbiol 41: 465-505
  12. Kooter JM, Matzke MA, Meyer P (1999) Listening to the silent genes: transgene silencing, gene regulation and pathogen control. Trends Plant Sci 4: 40-347
  13. Paszczynski A, Pasti-Grigsby MB, Goszczynski S, Crawford RL, Crawford DL (1992) Mineralization of sulfonated azo dyes and sulfanilic acid by phanerochaete chrysosporium and streptomyces chromofuscus. Appl Environ Microbiol 58: 3598-3600
  14. Scott SE, Wilkinson MJ (1999) Low probability of chloroplast movement from oilseed rape (Brassica napus) into wild Brassica rapa. Nature Biotechnol 17: 390-392
  15. Spadaro JT, Gold MH, and Renganathan V (1992) Degradation of azo dyes by the lignin-degrading fungus Phanerochaete chrysosporium. Appl Environ Microbiol 58: 2397-2401
  16. Streatfield SJ (2007) Approaches to achieve high-level heterologous protein production in plants. Plant Biotechnol J 5: 2-15

Cited by

  1. Stable expression of a fungal laccase protein using transplastomic tobacco vol.6, pp.4, 2012,