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Growth and Antioxidant Production of Bacillus polyfermenticus SCD in Whey Protein Concentrate (WPC)-based Medium

유청단백질농축물을 기본 배지로 한 Bacillus polyfermenticus SCD균의 생육과 항산화물질 생산

  • Choi, Gooi-Hun (Division of Animal Life Science, Konkuk University) ;
  • Lee, Jang-Hyun (Division of Animal Life Science, Konkuk University) ;
  • Jo, Mi-Na (Division of Animal Life Science, Konkuk University) ;
  • Yoon, Yo-Chang (Division of Animal Life Science, Konkuk University) ;
  • Paik, Hyun-Dong (Division of Animal Life Science, Konkuk University)
  • 최귀헌 (건국대학교 동물생명과학부) ;
  • 이장현 (건국대학교 동물생명과학부) ;
  • 조미나 (건국대학교 동물생명과학부) ;
  • 윤여창 (건국대학교 동물생명과학부) ;
  • 백현동 (건국대학교 동물생명과학부)
  • Published : 2008.03.30

Abstract

The cell growth and antioxidant activity of Bacillus polyfermenticus SCD were studied in tryptic soy broth (TSB) medium and whey protein concentrate (WPC)-based medium. Overall, higher lactose contents in WPC-35 medium (up to 2.0%), and longer culture times correlated with greater cell viability. In WPC-35 medium with 1.5% and 2.0% lactose, the cell growth of B. polyfermenticus SCD was similar to growth in TSB medium. The 1,1-diphenyl-2-picyrylhydrazyl (DPPH) radical scavenging activity of culture supernatant of B. polyfermenticus SCD in WPC-35 medium was measured to assess antioxidant activity. The antioxidant activity increased up to 32 hr of culture, reaching a maximum of 75.57% DPPH radical scavenging activity. The antioxidant activity seemed to follow the typical kinetics of primary metabolite synthesis. The antioxidant activity of B. polyfermenticus SCD supernatant in WPC-35 medium was more effective and stable than supernatant from TSB medium. These results suggest that WPC-35 medium is effective for the production of antioxidant by B. polyfermenticus SCD.

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