- Volume 18 Issue 3
DOI QR Code
Activities of Recombinant MT1-MMP Expressed in PANC-1 Cells.
PANC-1세포에서 발현된 재조합 MT1-MMP의 효소 활성
- Kim, Hye-Nan (Department of Biotechnology, Hannam University) ;
- Chung, Hye-Shin (Department of Biotechnology, Hannam University)
- Published : 2008.03.31
Membrane-type 1 matrix metalloproteinase (MT1-MMP) is a membrane-associated zinc-dependent endoproteinase involved in extracellular matrix remodeling. MT1-MMP hydrolyzes ECM proteins like collagen and is involved in cancer cell migration and metastasis. Caveolins are integral membrane proteins and play a role in formation of caveolae, specialized membrane microdomains involved in clathrin-independent endocytosis. Recombinant MT1-MMP was transiently expressed in PANC-1 cells. Cells expressing recombinant MT1-MMP were able to hydrolyze collagen and migrate on collagen coated trans-well. Both subjacent collagen degradation and the cell migration conferred by recombinant MT1-MMP were inhibited by co-transfection of plasmids containing caveolin-1 cDNA. The results support that MT1-MMP is localized in lipid raft of the membrane and MT1-MMP activities in invasive cells could be inhibited by caveolin.
- Liu, P., M. Rudick and R. G. W. Anderson. 2002. Multiple functions of Caveolin-1. J. Biol. Chem. 277, 41295-41298. https://doi.org/10.1074/jbc.R200020200
- Krajewska, W. M. and I. Maslowska. 2004. Caveolins: Struture and function in signal transduction. Cell Mol. Biol. Lett. 9, 195-220.
- Annabi, B., M. Lachambre, N. Bousqet-Gagnon, M.. Page, D. Gingras and R. Belivau. 2001. Localization of membrane- type 1 matrix metalloproteinase in caveolae membrane domain. Biochem. J. 353, 547-553. https://doi.org/10.1042/0264-6021:3530547
- Lieber M., J. Mazzetta, W. Nelson-Rees, M. Kaplan and G. Todaro. 1975. Establishment of a continuous tumor-cell line (panc-1) from a human carcinoma of the exocrine pancreas. Int. J. Cancer 15, 741-747. https://doi.org/10.1002/ijc.2910150505
- Holmbeck, K., P. J. Bianco, S. Caterina, S. Yamada, M. Kromer, S. A. Kuznetsov, M. Mankani, P. G. Robey, A. R. Poole, I. Pidoux, J. M. Ward and H. Birdedal-Hansen. 1999. MT1-MMP-deficient mice develop dwarfism, osteopenia, arthritis and connective tissue disease due to inadequate collagen turnover. Cell 99, 81-91. https://doi.org/10.1016/S0092-8674(00)80064-1
- Egenlad, M. and Z. Werb. 2002. New functions for the matrix metalloproteinases in cancer progression. Nat. Rev. Cancer 2, 161-174. https://doi.org/10.1038/nrc745
- Seiki, M. 2002. The cell surface: the stage for matrix metalloproteinase regulation of migration. Curr. Opin. Cell Biol. 14, 624-632. https://doi.org/10.1016/S0955-0674(02)00363-0
- Stanton, S., J. Gavrilovic, S. J. Atkinson, M. d'Ortho, K. M. Yamada, L. Zardi and G. Murphy. 1998. The activation of proMMP-2 (gelatinase A) by HT1080 fibrosarcoma cells is promoted by culture on a fibronectin substrate and is concomitant with an increase in processing of MT1-MMP (MMP14) to a 45kDa form. J. Cell Sci. 111, 2789-2798.
- Atkinsoon, S. J., M. L. Patterson, M. J, Butler and G. Murphy. 2001. Membrane type 1 matrix metalloproteinase and gelatinase A synergistically degrade type I collagen in a cell model. FEBS Lett. 491, 222-226. https://doi.org/10.1016/S0014-5793(01)02204-9
- Ohuchi, E., K. Imai, Y. Fujii, H. Sato, M. Seiki and K. Okada. 1997. Membrane type 1 matrix metalloproteinase digests interstitial collagens and other extracellular matrix macromolecules. J. Biol. Chem. 272, 2446-2451. https://doi.org/10.1074/jbc.272.4.2446
- Hottary, K. H., E. Allen, A. Punturieri, I. Yana and S. J. Weiss. 2000. Regulation of Cell Invasion and Morphogenesis in a Three-dimensional Type I Collagen Matrix by Membrane-type Matrix Metalloproteinases 1, 2, and 3. J. Cell Biol. 149, 1309-1323. https://doi.org/10.1083/jcb.149.6.1309