Antibacterial Activity of the Phaeophyta Ecklonia stolonifera on Methicillin-resistant Staphylococcus aureus

  • Eom, Sung-Hwan (Department of Food Science and Technology, Pukyong National University) ;
  • Kang, Min-Seung (Department of Food Science and Technology, Pukyong National University) ;
  • Kim, Young-Mog (Department of Food Science and Technology, Pukyong National University)
  • Published : 2008.03.31


In an effort to discover an alternative therapeutic agent against methicillin-resistant Staphylococcus aureus (MRSA), several medicinal plants and seaweeds were evaluated for its antibacterial activity against MRSA. A methanolic extract of the Phaeophyta Ecklonia stolonifera exhibited significant antibacterial activity against MRSA. To perform more detailed investigation on antibacterial activity, the methanol extract of E. stolonifera was further fractionated with organic solvents such as hexane, dimethylchloride, ethyl acetate, and n-butanol. Among them, the hexane fraction showed the strongest antibacterial activity against MRSA strains with MIC from 500 to $600 {\mu}g/mL$. The fraction also exhibited a bactericidal activity against MRSA, indicating that E. stolonifera contains a bactericidal substance against MRSA.



  1. Barna, J.C.J. and D.H. Williams. 1984. The structure and mode of action of glycopeptide antibiotics of the vancomycin group. Annu. Rev. Microbiol., 38, 339-357
  2. Finland, M. 1979. Emergence of antibiotic resistance in hospital 1935-1975. Rev. Infect. Dis., 1, 4-21
  3. Han, E.S., J.W. Kim,, M.O. Eom, I.H. Kang, H.J. Kang, J.S. Choi, K.W. Ha and H.Y. Oh. 2000. Inhibitory effects of Ecklonia stolonifera on gene mutation on mouse lymphoma tk+/- locus in L5178Y-3.7.2C cell and bone marrow micronuclei formation in ddY mice. Environ. Mutagen. Carcinogen., 20, 104-111
  4. Hanaki, H., H. Labischinski, T. Inaba, N. Kondo, H. Murakami and K. Hiramatsu. 1998. Increase in glu-tamine-non-amidated muropeptides in the peptidegly-can of vancomycin-resistant Staphylococcus aureus strain Mu50. J. Antimicrob. Chemother., 42, 315-320
  5. Hara, I. and T. Sakurai. 1998. Isolation and characteriza-tion of vanadium bromoperoxidase from a marine macroalga, Ecklonia stolonifera. J. Inorg. Biochem., 72, 23-28
  6. Hiramatsu, K., H. Hanaki, T. Ino, K. Yabuta, T. Oquri and P.C. Tenover. 1997. Methicillin-resistant Staphylo-coccus aureus clinical strain with reduced van-comycin susceptibility. J. Antimicrob. Chemother., 40, 135-136
  7. Joe, M.J., S.N. Kim, H.Y. Choi, W.S. Shin, G.M. Park, D.W. Kang and Y.K. Kim. 2006. The inhibitory effects of eckol and dieckol from Ecklonia stolonifera on the expression of matrix metalloproteinase-1 in human dermal fibroblasts. Biol. Pharm. Bull., 29, 1735-1739
  8. Kang, H.S., H.R. Kim, D.S. Byun, B.W. Son, T.J. Nam and J.S. Choi. 2004. Tyrosinase inhibitors isolated from the edible brown alga Ecklonia stolonifera. Arch. Pharm. Res., 27, 1226-1232
  9. Kang, H.S., H.Y. Chung, J.H. Jung, B.W. Son and J.S. Choi, 2003. A new phlorotannin from the brown alga Ecklonia stolonifera. Chem. Pharm. Bull., 51, 1012-1014
  10. Kim, Y.C., R.B. An, N.Y. Yoon, T.J. Nam and J.S. Choi. 2005. Hepatoprotective constituents of the edible brown alga Ecklonia stolonifera on tacrine-induced cytotoxicity in Hep G2 cells. Arch. Pharm. Res., 28, 1376-1380
  11. Kurata, K., K. Taniguchi, K. Shiraishi and M.M. Suzuki. 1993. Ecklonialactones-C-F from the brown alga Ecklonia stolonifera. Phytochemistry, 33, 155-159
  12. Lee, J.H., J.C. Park and J.S. Choi. 1996. The antioxidant activity of Ecklonia stolonifera. Arch. Pharm. Rev., 19, 223-227
  13. Lowy, F.D. 1998. Staphylococcus aureus infections. N. Engl. J. Med., 339, 520-532
  14. National Committee for Clinical Laboratory Standards. 1997. Method for Dilution Antimicrobial Suscepti-bility Test for Bacteria That Grow Aerobically, 4th ed., M7-A4. National Committee for Clinical Laboratory Standards, PA, USA
  15. Taniguchi, K., K. Kurata and M. Suzuki. 1991. Feeding-deterrent effect of phlorotannins from the brown alga Ecklonia stolonifera against the abalone Haliotis discus hannai. Nippon Suisan Gakkaishi, 57, 2065-2071
  16. Witte, W. 1999. Antibiotic resistance in Gram-positive bacteria: epidemiological aspects. J. Antimicrob. Chemoth., 44, 1-9

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