DOI QR코드

DOI QR Code

Physiological Activities of Dried Persimmon, Fresh Persimmon and Persimmon Leaves

곶감, 생감 및 감잎 추출물의 생리활성 효과

  • Hong, Jung-Hee (The Center for Traditional Microorganism Resources, Keimyung University) ;
  • Kim, Hyun-Jeong (The Center for Traditional Microorganism Resources, Keimyung University) ;
  • Choi, Yong-Hwa (Dept. of Plant Resources Sangju National University) ;
  • Lee, In-Seon (The Center for Traditional Microorganism Resources, Keimyung University)
  • 홍정희 (계명대학교 전통미생물자원개발 및 산업화연구(TMR)센터) ;
  • 김현정 (계명대학교 전통미생물자원개발 및 산업화연구(TMR)센터) ;
  • 최용화 (상주대학교 식물자원학과) ;
  • 이인선 (계명대학교 전통미생물자원개발 및 산업화연구(TMR)센터)
  • Published : 2008.08.30

Abstract

Antioxidative, antidiabetes, antibacterial, anticancer and angiotensin-converting enzyme (ACE) inhibitory activities of methanol extracts of dried persimmon, fresh persimmon and persimmon leaves were investigated. Total polyphenol content of dried persimmon, fresh persimmon and persimmon leaves were 147.79, 301.45 and $315.90\;{\mu}g/mg$, respectively, of which fresh persimmon and persimmon leaves had significantly higher total polyphenol than dried persimmon. Activities of DPPH radical scavenging, lipid peroxidation inhibition and salivary $\alpha$-amylase inhibition were increased in persimmon leaves related to total polyphenol contents. Anticancer activities against AGS of fresh persimmon and persimmon leaves were $65{\sim}70%$; however, there were no significant differences between dried persimmon and fresh persimmon on free radical scavenging activity and inhibitory activity of salivary $\alpha$-amylase. Also, extracts of dried persimmon, fresh persimmon and persimmon leaves showed good ACE inhibitory activities. Dried persimmon and fresh persimmon showed antibacterial activities on E.coli O157:H7. Therefore, there are many difference activities by dried and parts of persimmon. From this result, it is suggested that persimmon leaves is believed to have possible antioxidative, antidiabetes and anticancer capacities by polyphenol, but further studies on the identification of the active compound(s) as antioxidant, antidiabetic, antihypertensive and antibacterial materials will be needed to develop a better understanding of its potency on persimmons.

References

  1. Park SH, Hwang HS, Han JH. 2004. Development of drink from composition with medicinal plants and evaluation of its physiological function. Korean J Nutr 37: 364-372
  2. Mutsuo T, Itoo S, Ben-Arie R. 1991. A model experiment for elucidating the mechanism of astringency removal in persimmon fruit using respiration inhibitors. J Jpn Soc Hort Sci 60: 437-442 https://doi.org/10.2503/jjshs.60.437
  3. Yu TJ. 1976. Food carte . Pak Myoung Publishing Co., Seoul, Korea. p 129-132
  4. Hanlam E. 1981. Vegetable tannins. In Biochemistry of plants. Stumpf PK, Comm EE, eds. Academic Press, NY. p 527-539
  5. Nose M, Fujino N. 1982. Antioxidant activities of some vegetable food and active component of avocado epicarp. Nippon Shokuhin Kogyo Gakkaishi 29: 507-512 https://doi.org/10.3136/nskkk1962.29.9_507
  6. Seo JH, Jeong YJ, Kim KS. 2000. Physiological characteristics of tannins isolated from astringent persimmon fruits. Korea J Food Sci Technol 32: 212-217
  7. Moon SH, Kim KH, Park KY. 1996. Antitumor effect of persimmon leaves in vivo using sarcoma-180 cells. J Korean Soc Food Sci Nutr 25: 865-870
  8. Ma J, Liu XY, Noh KH, Kim MJ, Song YS. 2007. Protective effects of persimmon leaf and fruit extracts against acute ethanol-induced hepatotoxicity. J Korean Soc Food Sci Nutr 12: 202-208 https://doi.org/10.3746/JFN.2007.12.4.202
  9. Kim ES, Kim MK. 1999. Effect of dried leaf powders and ethanol extracts of persimmon, green tea and pine needle on lipid metabolism and antioxidative capacity in rats. Korean J Nutr 32: 337-352
  10. Ham YJ, Park YM. 2003. Evaluation of astringency removal process in carbon dioxide flushing system and storability of 'Sagoksi' persimmon fruits. J Kor Soc Hort Sci 44: 417-421
  11. Joung SY, Lee SJ, Sung NJ, Jo JS, Kang SK. 1995. The chemical composition of persimmon leaf tea. J Korean Soc Food Nutr 24: 720-726
  12. Song HS, Lee HK, Jang HD, Kim JI, Park OJ, Lee MS, Kang MH. 1996. Antimutagenic effects of persimmon leaf tea extracts in sister chromatid exchange (SCE) assay system. J Korean Soc Food Nutr 25: 232-239
  13. Moon SH, Park KY. 1995. Antimutagenic effects of boiled water extract and tannin from persimmon leaves. J Korean Soc Food Nutr 24: 880-886
  14. Park YJ, Kang MH, Kim JI, Park OJ, Lee MS, Jang HD. 1995. Changes of vitamin C and superoxide dismutase (SOD)-like activity of persimmon leaf tea by processing method and extraction condition. Korean J Food Sci Technol 27: 281-285
  15. Choi SW, Kang WW, Chung SK, Cheon SH, Rhuw TH. 1996. Antioxidative activity of flavonoids in persimmon leaves. Foodtech 5: 119-123
  16. Jeong HS, Chung HS, Lee HD, Seong JH, Choi JU. 2001. Controlled atmosphere storage and modified atmosphere packaging of astringency-removed persimmons. Food Sci Biotechnol 10: 380-386
  17. Kameda K, Takaku T, Okuda H, Kimura Y, Okuda T, Hatano T, Agata I, Arichi S. 1987. Inhibitory effects of various flavonoids isolated from leaves of persimmon on angiotensin converting enzyme activity. J Nat Prod 50: 680-683 https://doi.org/10.1021/np50052a017
  18. Singleton VL, Orthofer R, Lamuela-Raventos RM. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol 299: 152-178 https://doi.org/10.1016/S0076-6879(99)99017-1
  19. Moreno MI, Isla MI, Sampietro AR, Vattuone MA. 2000. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol 71: 109-114 https://doi.org/10.1016/S0378-8741(99)00189-0
  20. Blois MS. 1977. Antioxidant determinations by the use of a stable free radicals. J Agric Food Chem 25: 103-107 https://doi.org/10.1021/jf60209a051
  21. Slater TF, Sawyer BC. 1971. The stimulatory effects of carbon tetrachloride on peroxidative reactions in rat liver fractions in vitro. Interaction sites in the endoplasmic reticulum. Biochem J 123: 805-821 https://doi.org/10.1042/bj1230805
  22. Gutierrez AM, Reboredo GR, Arcemis CJ, Catala A. 2000. Non-enzymatic lipid peroxidation of microsomes and mitochondria isolated from liver and heart of pigeon and rat. Int J Biochem Cell Biol 32: 73-79 https://doi.org/10.1016/S1357-2725(99)00105-3
  23. Okhawa H, Ohishi N, Yagi K. 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95: 351-358 https://doi.org/10.1016/0003-2697(79)90738-3
  24. Ogawa S, Fujieda S, Sakata Y, Ishizaki M, Hisamatsu S, Okazaki K, Ooki Y, Mori M, Itoh M, Korenaga T. 2004. Synthesis and glycosidase inhibitory activity of some N-substituted 5a-carba-$\beta$-fuco- and $\beta$-galactopyranosylamines, and selected derivatives. Bioorg Med Chem 12: 6569-6579 https://doi.org/10.1016/j.bmc.2004.09.023
  25. Gao H, Kawabata J. 2005. $\alpha$-Glucosidase inhibition of 6-hydroxyflavones. Part 3: Synthesis and evaluation of 2,3,4-trihydroxybenzoyl-containing flavonoid analogs and 6-aminoflavones as $\alpha$-glucosidase inhibitors. Bioorg Med Chem 13: 1661-1671 https://doi.org/10.1016/j.bmc.2004.12.010
  26. Cushman DW, Cheung HS. 1971. Spectrophotometric assay and properties of the angiotensin converting enzyme of rabbit lung. Biochem Pharmacol 20: 1637-1648 https://doi.org/10.1016/0006-2952(71)90292-9
  27. Green LM, Reade JL, Ware CF. 1984. Rapid colorimetric assay for cell viability: application to the quantitation of cytotoxic and growth inhibitory lymphokines. J Immunol Methods 70: 257-268 https://doi.org/10.1016/0022-1759(84)90190-X
  28. Lee TB. 1979. I llustrated Flora of Korea. Hyangmoon Publishing Co., Seoul, Korea. p 511
  29. Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1198-1200 https://doi.org/10.1038/1811199a0
  30. Ancerewicz J, Migliavacca E, Carrupt PA, Testa B, Bree F, Zini R, Tillement JP, Labidalle S, Guyot D, Chauvet-Monges AM, Crevat A, Le Ridant A. 1998. Structure-property relationships of trimetazidine derivatives and model compounds as potential antioxidants. Free Radic Biol Med 25: 113-120 https://doi.org/10.1016/S0891-5849(98)00072-0
  31. Kang YH, Park YK, Oh SR, Mood KD. 1995. Studies on the physiological functionality of pine needle and mugwort extracts. Korean J Food Sci Technol 27: 978-984
  32. Uchida S, Ohta H, Niwa M, Mori A, Nonaka G, Nishioka I, Ozaki M. 1990. Prolongation of life span of stroke-prone spontaneously hypertensive rats (SHRSP) ingesting persimmon tannin. Chem Pharm Bull (Tokyo) 38: 1049-1052 https://doi.org/10.1248/cpb.38.1049
  33. Ito S, Oshima Y. 1962. Studies on the tannin of Japanese persimmon. Agric Biol Chem 26: 156-161 https://doi.org/10.1271/bbb1961.26.156
  34. Torel J, Cillard J, Cillard P. 1986. Antioxidant activity of flavonoids and reactivity with peroxy radical. Phytochemistry 25: 383-385 https://doi.org/10.1016/S0031-9422(00)85485-0
  35. Josse RG, Chiasson JL, Ryan EA, Lau DC, Ross SA, Yale JF, Leiter LA, Maheux P, Tessier D, Wolever TM, Gerstein H, Rodger NW, Dornan JM, Murphy LJ, Rabasa-Lhoret R, Meneilly GS. 2003. Acarbose in the treatment of elderly patients with type 2 diabetes. Diabetes Res Clin Pract 59: 37-42 https://doi.org/10.1016/S0168-8227(02)00176-6
  36. Matsui T, Ueda T, Oki T, Sugita K, Terahara N, Matsumoto K. 2001. $\alpha$-Glucosidase inhibitory action of natural acylated anthocyanins. 1. Survey of natural pigments with potent inhibitory activity. J Agric Food Chem 49: 1948-1951 https://doi.org/10.1021/jf001251u
  37. Queseda C, Bartolome B, Nieto O, Gomez-Cordoves C, Hernanadez T, Estrella I. 1995. Phenolic inhibitors of $\alpha$-amylase and trypsin enzymes by extracts from pears, lentils and cocoa. J Food Prot 59: 185-192
  38. Gyemant G, Zajacz A, Batta G. 2004. Inhibitory effects of tannin on human salivary alpha-amylase. Biochem Biophys Res Commun 319: 1265-1271 https://doi.org/10.1016/j.bbrc.2004.05.122
  39. Lee WY, Ahn JK, Park YK, Park SY, Kim YM, Rhee HI. 2004. Inhibitory effects of proanthocyanidin extracted from Distylium racemosum on $\alpha$ -amylase and $\alpha$-glucosidase activities. Kor J Pharmacogn 35: 271-275
  40. Noh H, Song KB. 2001. Isolation of an angiotensin converting enzyme inhibitor from Oenathe javanica. Agric Chem Biotechnol 44: 98-99
  41. Oh SJ, Kim SH, Kim SK, Baek YJ, Cho KH. 1997. Angiotensin I-converting enzyme inhibitory activity of the K-casein fragments hydrolyzated by chymosin, pepsin, and trysin. Fractionation of angiotensin converting enzyme (ACE) inhibitory peptides from soybean paste. Korean J Food Sci Technol 27: 230- 234
  42. Funayama S, Hikino H. 1979. Hypotensive principles of Diospyros kaki leaves. Chem Pharm Bull (Tokyo) 27: 2865-2868 https://doi.org/10.1248/cpb.27.2865
  43. Hibasami H, Achiwa Y, Fujikawa T, Komiya T. 1996. Induction of programmed cell death (apoptosis) in human lympoid leukemia cells by catechin compounds. Anticancer Res 16: 1943-1946
  44. Nose K. 1984. Inhibition of flavonoids of RNA synthesis in permeable WI-38 cells and of transcription by RNA polymerase II. Biochem Pharmacol 33: 3823-3837 https://doi.org/10.1016/0006-2952(84)90046-7
  45. Moon SH. 2002. Inhibitory effect of persimmon leaves on the mutagenicity in spore rec assay and on the growth of human cancer cells. Korean J Food & Nutr 15: 23-28
  46. Ueda S, Yamashita H, Nakajima M, Kuwabara Y. Inhibition of microorganism by spice extracts and flavoring compounds. Nippon Shokuhin Kogyo Gakkaishi 29: 389-392
  47. Kim MH, Lee NH, Lee MH, Kwon DJ, Choi UK. 2007. Antimicrobial activity of aqueous ethanol extracts of Perilla frutescens var. acuta leaf. Korean J Food Culture 22: 266-273

Cited by

  1. Effect of Removed Peel from Sweet Persimmon on Nutritional Ingredients and Antioxidant Activities vol.39, pp.10, 2010, https://doi.org/10.3746/jkfn.2010.39.10.1495
  2. Isolation and Identification of Contaminated Organisms on Dried Persimmon vol.19, pp.6, 2012, https://doi.org/10.11002/kjfp.2012.19.6.939
  3. Antioxidative and Antiallergic Effect of Persimmon Leaf Extracts vol.38, pp.12, 2009, https://doi.org/10.3746/jkfn.2009.38.12.1691
  4. Antioxidant Activity and α-Glucosidase Inhibitory Effect of Jerusalem Artichoke (Helianthus tuberosus) Methanol Extracts by Heat Treatment Conditions vol.19, pp.4, 2011, https://doi.org/10.7783/KJMCS.2011.19.4.257
  5. Anti-diabetic and Anti-oxidative Effects of Opuntia humifusa Cladodes vol.43, pp.5, 2014, https://doi.org/10.3746/jkfn.2014.43.5.661
  6. Physical Characteristics and Changes in Functional Components of Gochujang with Different Amounts of Sweet Persimmon Powder vol.40, pp.12, 2011, https://doi.org/10.3746/jkfn.2011.40.12.1668
  7. Effect of freezing temperature on quality characteristics of dried persimmons vol.23, pp.7, 2016, https://doi.org/10.11002/kjfp.2016.23.7.931
  8. Antioxidative and Antidiarrheal Effects of Persimmon Extracts vol.26, pp.4, 2010, https://doi.org/10.5625/lar.2010.26.4.407
  9. Physicochemical Characteristics and Antioxidant Activity of Bracken (Pteridium aquilinum Kuhn) in Namhae vol.31, pp.3, 2015, https://doi.org/10.9724/kfcs.2015.31.3.288
  10. Evaluation of Physicochemical Properties and Enhancement of Antioxidant Activities of Dioscorea batatas by Stepwise Steaming Process vol.25, pp.6, 2015, https://doi.org/10.17495/easdl.2015.12.25.6.1049
  11. Antioxidant Activity of Branches of Fuyu and Nishimurawase Persimmon Trees vol.53, pp.2, 2010, https://doi.org/10.3839/jabc.2010.015
  12. The Antioxidant and Skin Whitening Effect of Artemisia iwayomogi Extracts vol.44, pp.1, 2012, https://doi.org/10.9721/KJFST.2012.44.1.089
  13. Biological Activities of Extracts from Gamma-irradiated Aralia elata Cortex vol.43, pp.8, 2014, https://doi.org/10.3746/jkfn.2014.43.8.1236
  14. Antioxidative Activities and Quality Characteristics of Steamed Roll with Added Ligularia fischeri (Ledeb.) Turcz. Powder vol.32, pp.2, 2016, https://doi.org/10.9724/kfcs.2016.32.2.147
  15. Anti-aging and Anti-diabetes Effects of Aconitum pesudo-laeve var. erectum Extracts vol.23, pp.5, 2013, https://doi.org/10.5352/JLS.2013.23.5.616
  16. Studies on the Anti-Oxidative and Anti-Inflammatory Activity of the Extracts from Persimmon Waste vol.34, pp.4, 2018, https://doi.org/10.9724/kfcs.2018.34.4.335