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The Stabilization of Lactic Acid Bacteria, Bacillus polyfermenticus KJS-2

유산간균인 Bacillus polyfermenticus KJS-2의 안정화

  • 김강민 (인제대학교 제약공학과) ;
  • 이진영 (인제대학교 제약공학과) ;
  • 홍용근 (인제대학교 물리치료학과) ;
  • 이상길 (인제대학교 제약공학과) ;
  • 강재선 (인제대학교 제약공학과)
  • Published : 2008.08.30

Abstract

Bacillus polyfermenticus KJS-2 (Bp2) was isolated from $Bispan^{(R)}$, a commercially available probiotics consisting of more than 4 strains. The objective of this study was to investigate the effect of three-layer coating on the stabilty of Bp2. Bp2 was microencapsulated with sodium alginate using an air atomizer. The Bp2 loaded pellets were also coated with HFP-chitosan and HPMCP for oral delivery system. When compared to the uncoated Bp2, the survival of the three-layer coated Bp2 increased to approximately 63% (p<0.01) in a 30% ethanol solution, 54% (p<0.05) in an artificial gastric juice (pH 2), and 53% (p<0.05) in the bile acid (pH 5). When coated beads were stored at $100^{\circ}C$ and $130^{\circ}C$, Bp2 in coated beads was very stable (p<0.01) compared to uncoated Bp2.

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