Adsorption Isotherms of 2-deoxyuridine (dUrd) and 2-deoxycytidine (dCyd) by Static Method

정적 방법에 의한 2-deoxyuridine(dUrd)과 2-deoxycytidine(dCyd)의 흡착 평형식

  • Published : 2008.02.28

Abstract

Adsorption isotherm with the most fundamental information related to chromatography process is obtained experimentally. The adsorption isotherm of 2-deoxyuridine (dUrd) and 2-deoxycytidine (dCyd) with ${\mu}$-Bondapak $C_{18}$, static method was adopted in RP-HPLC. The concentrations of mobile and stationary phases were measured with different initial concentrations of dUrd and dCyd, 1, 3, 5, 7, 10 mg/mL, respectively. The adsorption isotherm data were applied by Freundlich, Langmuir, Sips, and Radke-Prausnitz model equations. As a result of the regression analysis, standard error between adsorption isotherm of dUrd and Radke-Prausnitz equation was very low, and adsorption isotherm of dCyd was in an agreement with Sips equation very well.

Keywords

static method;adsorption isotherm;2-deoxyuridine (dUrd);2-deoxycytidine (dCyd);HPLC

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