Inhibition of Meloidogyne incognita Egg Hatching by Herbal Extracts

식물체 추출물이 뿌리혹선충(Meloidogyne incognita)의 부화에 미치는 영향

  • Elbadri, G.A.A. (Agricultural Research Corporation, Cop Protection Research Center) ;
  • Lee, Dong-Woon (Department of Applied Biology, Kyungpook National University) ;
  • Park, Jung-Chan (Department of Applied Biology and Environmental Sciences, Gyeongsang National University) ;
  • Choo, Ho-Yul (Department of Applied Biology and Environmental Sciences, Gyeongsang National University) ;
  • Kim, Hyeong-Hwan (Horticultural Environment Division, National Horticultural Research Institute)
  • ;
  • 이동운 (경북대학교 생물응용학과) ;
  • 박정찬 (경상대학교 응용생물환경학과) ;
  • 추호렬 (경상대학교 응용생물환경학과) ;
  • 김형환 (원예연구소 원예환경과)
  • Published : 2008.06.30


The inhibitory effect of herbal extracts using methanol and hexane collected from Sudan and Korea was evaluated on egg hatching of Meloidogyne incognita. The concentration of herbal extracts were 10,000, 1,000 and 100 ppm, respectively. They were treated to Meloidogyne incognita eggs after 3, 7, and 21 days of hatching. All herbal extracts inhibited egg hatching significantly compared to the control. The degree of inhibition was directly related to the concentration of the extracts. At 10,000 ppm, very fewer individuals were hatched at all the periods of exposure with the exception of the extract of fruits of Quisqalis indica which gave 84.4 and 54.5% at 7 and 21 days, respectively. Egg hatching was completely ceased, especially after 7 days for most of the extracts. While at 1,000 ppm relatively high numbers of eggs were hatched compared to the higher concentration, as well the extracts Q. indica reported many number of hatched eggs compared to others, especially at 21 days. On the other hand, the extract from pods of Cucumis mello var agrestis gave the least hatching number of eggs at all periods of exposure which gave 2, 8, and 3% at 3, 7, and 21 days, respectively in comparison to the control. Whereas at 100 ppm, inhibition increased with exposure time. However, the extract leaf of Desmodium caudatum represented the fewer hatching eggs at 3 and 7 days.


  1. Choi, D. R. and Y. E. Choi (1982) Survey on plant parasitic nematodes in cropping by controlled horticulture. The Korean J. Plant Protec. 21:8-14
  2. Coxa, J. C., L. B. McCartya, J. E. Torleb, S. A. Lewis and S. B. Martine (2006) Suppressing sting nematodes with Brassica sp. and spotted spurge extracts. Agronomy Journal 98:962-967
  3. Duke, S. O. (1990) Natural pesticides from plants. pp. 511-517, In Advances in new crops (eds. Janick, J. and J. E. Simon), Timber Press, Portland
  4. Eisenback, J. D. and H. Hirschmann (1991) Root-not nematodes: Meloidogyne species and races. pp. 191-274, In Manual of agricultural nematology (eds. Nickle., W. R., and Marcel Dekker). New York
  5. Kim, D. I., J. D. Park, S. G. Kim, H. Kuk, M. J. Jang and S. S. Kim (2005) Screening of some crude plant extracts for their acaricidal and insecticidal efficacies. J. Asia-Pacific Entomol. 8:93-100
  6. Lee, H. S., G. J. Choi, K. Y. Cho, S. G. Lee and Y. J. Ahn (2000) Fungicidal and insecticidal activities of various grain extracts against five insect pest pests and six phytopathogenic fungi. The Korean Journal of Pesticide Science 4:7-14
  7. Lee, S. G., B. S. Park, S. E. Lee, J. G. Son, C. Song and H. S. Lee (2001b) Toxicity of various fruit trees extracts to five agricultural and four store-product arthropods pests. The Korean Journal of Pesticides Science, 4:27-32
  8. Onifade, A. K. (2007) Effect of essential oils from five Ocimum sp. on the pathogenicity of Pratylenchus brachyurus (Godfery) in tomato. Agricultural Journal 2:185-191
  9. Osman, A. A and D. Viglierchio (1988) Efficacy of biologically active agents as nontraditional nematicides for Meloidogyne javanica. Revue Nematol. 11:93-98
  10. Lee, S. G., Y. J. Ahn, J. D. Park, J. C. Kim, K. Y. Cho and H. S. Lee (2001a) Fungicidal activity of 46 plant extracts against rice leaf blast, rice sheath blight, tomato late blight, cucumber gray mold, barley powdery mildew and wheat leaf rust. The Korean Journal of Pesticide Science 5:18-25
  11. SAS. (2004) SAS Institute Onlinedoc $\left(R \right)$ version 8.01 Statistical Analysis System Cary, NC. USA
  12. Meyer, S.L. F., I. A. Zasada, D. P. Roberts, B. T. Vinyard, D. K. Lakshman, J. K. Lee, D. J. Chitwood and L. K. Carta (2006) Plantago lanceolata and Plantago rugelii extracts are toxic to Meloidogyne incognita but not to certain microbes. Journal of Nematology, 38:333-338
  13. Yassin, A. M. (1984) Root-knot nematodes problems on vegetables in the Sudan. Acta Hort. 143:407-416
  14. Pandey, R. (2000) Additive effect of three organic materials and some nematicides on the reproduction of Meoidogyne incognita and yield of Mentha arvensis. Nematropica 30:155-160
  15. Pandey, R. and A. Kalra (2005) Chemical activators: A novel and sustainable management approach for Meloidogyne incognita (Kofoid and White) Chitwood in Chamomilla recutita L. Archives of Phytopathology and Plant Protection 38:107-111
  16. Carter, C. C. and J. N. Sasser (1982) Research on the integrated crop protection system with emphasis on the root-knot nematodes (Meloidogyne spp.) affecting economic food crops in developing nations. International Meoidogyne Project (IMP) contract No. AID/ta-c-1234. A cooperative publication of the department of plant pathology North Carolina State University and United States Agency for International Development
  17. Musabyimana, T. and R. C. Saxena (1999) Efficacy of neem seed derivatives against nematodes affecting banana. Phytoparasitica 27:43-49
  18. Lee, H. S., B. S. Kim, T. H. Kim, K. Y. Cho and Y. J. Ahn (1998) Fungicidal activities of leguminous seed extracts toward phytopathogenic fungi. The Korean Journal of Pesticide Science 2:21-27
  19. Piotte, C., P. Castagnone-Sereno, J. Uijhof, P. Abad, M. Bongiovanni and A. Dalmasso (1992) Molecular characterization of species and populations of Meloidogyne from various geographic origins with repeated DNA homologous probes. Fundamental of Applied Biology 15:271-276
  20. Kong, J. O., S. M. Lee, Y. S. Moon, S. G. Lee and Y. J. Ahn (2007) Nematicidal activity of cassia and cinnamon oil compounds and related compounds toward Bursaphylenchus xylophilus (Nematoda: Parasitaphelenchidae). Journal of Nematology 39:31-36
  21. Husan-Bano, I. A. Siddiqui, W. Ahmed and S. Ehteshamul-Hague (1999) Effect of some medicinal plants of Meloidogyne javancica, root-knot nematodes. Pakistan Journal of Biological Sciences 2:1364-1365
  22. Castagone-Sereno, P., F. Vanlerberghe-Masutti and F. Leroy (1994) Genetic polymorphism between and within Meloidogyne species detected with RAPD markers. Genome 37:904-909
  23. Jepson, S. B. (1987) Identification of root-knot nematodes (Meloidogyne species). Wallingford, UK, C. A .B. International
  24. Yassin, A. M., E. M. Elamin and H. Decker (1992) The Impact of plant parasitic nematodes on major food crops in the Sudan. In: The biology and control of nematodes pests of food crops in Africa (eds. Fawole, B., O. A. Egunjiobi, S. O. Adesiyan, O. Batabola and A. A. Idowu), Proceedings of the first regional symposium on the biology and control of nematodes pests in food crops in Africa. University of Ibadan, Nigeria 26-29, July, 1992
  25. Zasada, I. A., W. Klassen, S. L. F. Meyer, M. Codallo and A. A. Abdul-Baki (2006) Velvet bean (Munca pruriens) extracts; impact on Meloidogyne incognita survival and on Lycopersicon esculentum and Lactuca sativa germination and growth. Pest management Sciences 62:1122-1127
  26. Park, I. K., S. G. Lee, J. D. Park, S. C. Shin and Y. J. Ahn (2003) Fungicidal activity of domestic plant extracts against six major phytopathogenic fungi. The Korean Journal of Pesticide Science 7:83-91
  27. Menna, S. and H. Melakeberhan (2006) Comparative efficacy of oil seed radish and tomato root exudates on hatching of Meloidogyne hapla. Pakistan Journal of Biological Sciences 6:485-489
  28. Nickle, W. R. (1991) A manual of agricultural nematology. 1035 pp. Marcel Dekker Inc., New York
  29. Amer-Zareen, M. J. Zaki and N. Javed (2003) Nematicidal activity of Ginger and its effect on the efficacy of Pasteuria penetrans for the control of root-knot nematodes on tomato. Asian Journal of Plant Sciences 2:858-860
  30. Shaukat, S. S. and I. A. Siddiqui (2001) Nematicidal activity of some weed extracts against Meloidogyne javanica (Treub.) Chitwood. Pakistan Journal of Biological Sciences 4:1251-1252
  31. Bello, L., P. Chindo, P. Marley and M. Alegbeijo (2006) Effect of some plant extracts on larval hatch of the root-knot nematodes, Meloidogyne incognita. Archives of Phytopathology and Crop Protection 39:253-257
  32. Cho, M. R., B. C. Lee, D. S. Kim, H. Y. Jeon, M. S. Yiem and J. O. Lee (2000) Distribution of plant-parasitic nematodes in fruit vegetable production areas in Korea and identification of root-knot nematodes by enzyme phenotypes. Korean J. Appl. Entomol. 39:123-129
  33. Orion, D., G. Kritzman, S. L. F. Meyer, E. F. Erbe and D. J. Chitwood (2001) A role of the gelatinous matrix in the resistance of root-knot nematodes (Meloidogyne spp) eggs to microorganisms. Journal of Nematology 33:203-207