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Comparison of Panning and Sodium Polytungstate Methods for Separating Dinoflagellate Cysts

와편모조류 시스트 분리를 위한 Panning 방법과 SPT 용액방법의 비교

  • Published : 2008.06.30

Abstract

Two methods for separating dinoflagellate cysts from marine sediments were compared: panning and sodium polytungstate (SPT). After pretreatment with the SPT solution, Protoperidinium species had a high germination rate and cell growth rate. The germinated cells grew continuously, even in a low nutrient environment. Alexandrium cysts did not germinate, because their germination is usually active in winter. Using SPT solution, the separation process was easier, quicker, and caused much less eye fatigue than the panning method. Therefore, SPT solution offers a convenient way of pretreating dinoflagellate cysts for separation and analysis.

References

  1. Anderson, D.M. and J.J. Lively. 1985. Sinking charac- teristics of dinoflagellate cysts. Limnol. Oceanogr., 30, 1000-1009 https://doi.org/10.4319/lo.1985.30.5.1000
  2. Iwakaki, H. 1961. The life cycle of Porphyra tenera in vitro. Biol. Bull., 121, 173-187 https://doi.org/10.2307/1539469
  3. Kim, Y.O., M.H. Park and M.S. Han. 2002. Role of cyst germination on the bloom initiation of Alexandrium tamarense (Dinophyceae) in Masan Bay, Korea. Aquat. Microb. Ecol., 29, 279-286 https://doi.org/10.3354/ame029279
  4. Robinson, S.M.C. and R.A. Chandler. 1993. An effective and safe method for sorting small molluscs from sediment. Limnol. Oceanogr., 38, 1088-1091 https://doi.org/10.4319/lo.1993.38.5.1088
  5. Savage, N.M. 1988. The use of sodium polytungstate for conodont separations. J. Micropalaeon., 7, 39-40 https://doi.org/10.1144/jm.7.1.39
  6. Shin, J.B. 2002. Distribution and germinability of Alexandrium tamarense/catenella resting cysts for the prediction of toxic algal blooms. Master thesis, Pukyong National University, Korea, 1-46
  7. Six, J., P.A. Schultz, J.D. Jastrow and R. Merckx. 1999. Recycling of sodium polytungstate used in soil organic matter studies. Soil Biol. Biochem., 31, 1193-1196 https://doi.org/10.1016/S0038-0717(99)00023-1
  8. Park, C.M. 2000. Distribution and germination charac- teristics of the resting cysts of toxic dinoflagellate genus Alexandirum in Korean coastal waters. Master thesis, Pukyong National University, Korea, 1-98
  9. Kremp A. and D.M. Anderson. 2000. Factors regulating germination of resting cysts of the spring bloom dinoflagellate Scrippsiella hangoei from the northern Baltic Sea. J. Plank. Res., 22, 1311-1327 https://doi.org/10.1093/plankt/22.7.1311
  10. Gregory, M.R. and K.A. Johnston. 1987. A non-toxic substitute for hazardous heavy liquids-aqueous sodium polytungstate (3$Na_2$$WO_4$9$WO_3$.$H_2O$) soluion. N.Z. J. Geol. Geophys., 30, 317-320 https://doi.org/10.1080/00288306.1987.10552626
  11. Matsuoka, K., Y. Fukuyo and D.M. Anderson. 1989. Methods for modern dinoflagellate cyts studies. In: Red Tides: Biology, Environmental Science and Toxicology. Elsevier Science Publishing, Tokyo, Japan, 461-479
  12. Park, M.H, Y.O. Kim, S.Y. Cho, M.S. Han. 2004. Effect of environmental conditions on germination of Alexandrium tamarense cysts from Masan Bay, Korea. Kor. J. Environ. Biol., 22, 200-205
  13. Bolch, C.J.S. 1997. The use of sodium polytungstate for the separation and concentration of living dino-flagelalte cysts from marine sediments. Phycologia, 36, 472-478 https://doi.org/10.2216/i0031-8884-36-6-472.1
  14. Anderson, D.M. and B.A. Keafer. 1987. An endogenous annual clock in the toxic marine dinoflagellate Gonyaulax tamarensis. Nature, 325, 616-617 https://doi.org/10.1038/325616a0
  15. Anderson, D.M. 1980. Effects of temperature conditioning on development and germination of Gonyalulax tamarensis (Dinophyceae) hypnozygotes. J. Phycol., 16, 166-172 https://doi.org/10.1111/j.1529-8817.1980.tb03013.x