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Bio-inspired Cell Deformability Monitoring Chips Based on Strain Dependent Digital Lysis Rates

미소유로의 길이에 따른 통과세포의 파괴율을 바탕으로 한 생체모사 세포 변형성 검사칩에 관한 연구

  • 윤세찬 (한국과학기술원 바이오시스템학과, 디지털나노구동연구단) ;
  • 이동우 (한국과학기술원 바이오시스템학과, 디지털나노구동연구단) ;
  • 조영호 (한국과학기술원 바이오시스템학과 및 기계공학과, 디지털나노구동연구단)
  • Published : 2008.10.01

Abstract

We present a novel cell deformability monitoring chip based on the digitally measured cell lysis rate which is dependent on the areal strain of the cell membrane. This method offers simple cell deformability monitoring by automated high-throughput testing system. We suggest the filter design considering the areal strain imposed on the cell membrane passing through the filter array having gradually increased orifice length. In the experiment using erythrocytes, we characterized the cell deformability in terms of average fracture areal strain which was $0.24{\pm}0.014\;and\;0.21{\pm}0.002$ for normal and chemically treated erythrocytes, respectively. We also verified that the areal strain of 0.15 effectively discriminates the deformability difference of normal and chemically treated erythrocytes, which can be applied to the clinical situation. We compared the lysis rates and their difference for the samples from different donors and found that the present chips can be commonly used without any calibration process. The experimental results demonstrate the simple structure and high performance of the present cell deformability monitoring chips, applicable to simple and cost-effective cell aging process monitoring.

Keywords

Cell Deformability;Erythrocyte Deformability;Cell Lysis;Red Blood Cell

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