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The effects of Ganoderma lucidum herba pharmacopuncture on 3T3-L1 preadipocyte differentiation

3T3-L1 지방전구세포의 분화에 미치는 영지약침의 영향

  • Lee, Chea-Woo (Dept. of Acupuncture & Moxibustion College of Oriental Medicine, Dong-Eui University) ;
  • Yoon, Hyun-Min (Dept. of Acupuncture & Moxibustion College of Oriental Medicine, Dong-Eui University) ;
  • Kang, Kyung-Hwa (Department of Oriental Physiology, College of Oriental Medicine, Dongeui University)
  • 이채우 (동의대학교 한의과대학 침구학교실) ;
  • 윤현민 (동의대학교 한의과대학 침구학교실) ;
  • 강경화 (동의대학교 한의과대학 생리학교실)
  • Published : 2008.09.30

Abstract

Objective: The purpose of this study is to investigate the effects of Ganoderma lucidum herba pharmacopuncture(GHP) on the adipogenesis in 3T3-L1 preadipocytes. Methods: 3T3- L1 preadipocytes were differentiated with adipogenic reagents by incubating for 2 days in the absence or presence of GHP ranging from 1 and 2%. The effect of GHP on cell proliferation of 3T3-L1 preadipocytes was investigated using MTT assay. The effect of GHP on adipogenesis was examined by Oil red O staining and measuring glycerol-3-phosphate dehydrogenase (GPDH) and intracellular triglyceride (TG) content. Results: Following results were obtained from the preadipocyte proliferation and adipocyte differentiation of 3T3-L1. We observed no effect of GHP on preadipocyte proliferation. GHP inhibited adipogenesis, the activity of GPDH and accumulation of intracellular TG content. Conclusions: These results suggest that GHP inhibit differentiation of preadipocyte.

Keywords

References

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