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A study on the comparison of antioxidant effects among wild ginseng, cultivated wild ginseng, and cultivated ginseng extracts

자연산 산삼, 산양삼 및 인삼의 항산화능 비교연구

  • Jang, Hae-Young (Department of Acup & Moxi, Korean Medical College, Sangji University) ;
  • Park, Hee-Soo (Department of Acup & Moxi, Korean Medical College, Sangji University) ;
  • Kwon, Ki-Rok (Department of Acup & Moxi, Korean Medical College, Sangji University) ;
  • Rhim, Tae-Jin (Devision of Animal resourses and life science, Sangji University)
  • 장해영 (상지대학교 한의과대학 침구학교실) ;
  • 박희수 (상지대학교 한의과대학 침구학교실) ;
  • 권기록 (상지대학교 한의과대학 침구학교실) ;
  • 임태진 (상지대학교 응용동물과학부)
  • Published : 2008.09.30

Abstract

Objective: The objective of this study was to compare the antioxidant effects among wild ginseng, cultivated wild ginseng, and ginseng extracts. Methods: In vitro antioxidant activities were examined by total antioxidant capacity (TAC), oxygen radical scavenging capacity(ORAC), total phenolic content, 1, 1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, inhibition of induced lipid peroxidation using liver mitochondria, reactive oxygen species(ROS) scavenging effect using 2', 7'-dichlorofluorescein(DCF) fluorescence. Results: 1. TAC of 1.5 and 3.75 mg extracts was highest in cultivated wild ginseng, followed by wild ginseng and lowest in ginseng. 2. ORAC of 2, 10, and $20{\mu}g$ extracts was highest in cultivated wild ginseng, followed by wild ginseng and lowest in ginseng. 3. Total phenolic content of 0.375, 0.938, and 1.875 mg extracts was highest in cultivated wild ginseng, followed by wild ginseng and lowest in ginseng. 4. DPPH(1, 1 -Diphenyl-2-picrylhydrazyl) scavenging activity between wild ginseng and cultivated wild ginseng did not differ significantly (p>0.05). 5. Induced lipid peroxidation, measured by TBARS concentration in solution containing rat liver mitochondria incubated in the presence of $FeSO_4$/ascorbic acid was inhibited as amounts of wild ginseng, cultivated wild ginseng, and ginseng extracts increased. TBARS concentration of ginseng extracts were significantly (p<0.05) higher than wild ginseng or cultivated wild ginseng extracts. 6. DCF fluorescence intensity was decreased as concentrations of wild ginseng, cultivated wild ginseng, and ginseng extracts increased, demonstrating that ROS generation was inhibited in a concentrationdependent manner. Conclusions: In summary, the results of this study demonstrate that cultivated wild ginseng extracts had similar antioxidant activities to wild ginseng extracts and greater that of cultivated ginseng extracts.

References

  1. M. Valko, D. Leibfritz, J. Moncol, Mark T.D. Cronin, M. Mazur, J. Telser. Free radicals and antioxidant in normal physiological funtions and human disease. The International J. & Cell Biology. 2007;39:44-84 https://doi.org/10.1016/j.biocel.2006.07.001
  2. Sawyer, DT., Valentine, JS. How super is superoxide? ACC. Chem. Res. 1981;14:393 https://doi.org/10.1021/ar00072a005
  3. Fridorich, I. Biological effects of the superoxide radical. Arch. Biophys. 1986;247:1-11 https://doi.org/10.1016/0003-9861(86)90526-6
  4. Ames, BN. Dietary carcinogens and anticarcinogens. Oxygen radical and degenerative disease. Science. 1983; 221:1256-1264 https://doi.org/10.1126/science.6351251
  5. Chance, B., Sies, H., Boveris A. Hydroperoxide metabolism in mammalian organs. Physiol. Rev. 1979;59:527
  6. Kandawami, C., Middleton, EJ. Free radical scavenging and anti-oxident activity of plant flavonoids. Free Radicals in Diagnostic Medicine. Armstrong D. Plenum Press, New York and London. 1994;351-376
  7. 하대유. 인삼에 대한 세포학 및 면역학적 연구. 대한면역학회지. 1979;1(1):45-52
  8. 山田昌之. 朝鮮人蔘의 硏究. 日本藥理學會誌. 1955;51:390
  9. Brekhman. I.I, Panax ginseng, Gosudarst Isdat et Med, Lit. Leningard, 1957;1
  10. 최진호. 인삼의신비. 교문사. 1984;13-14
  11. 남기열. 최신 고려인삼. 천일인쇄소. 1996;56
  12. Takagi, K, Proceedings International Ginseng Symposium, The Central Research Institute, Office of Monopoly, Seoul, Korea, 1974;119
  13. 김승환, 장명제, 이성규, 장완성, 최현희, 성종환. 인삼복용시 과산화지질과 총항산화능에 미치는 영향. 한국체육학회지. 2003;42(3):661-668
  14. Choi, KJ., Kim, MW., Hong, SK., Kim, DH. Effect of solvents on the yield, brown color intensity, UV absorbance, reducing and antioxident activities of extracts from white and red ginseng. J. Korean Agric. Chem. Soc. 1983;26:8-18
  15. 이승은, 이성우, 방진기, 유영주, 성낙술. 인삼의 부위별항산화활성. Korean J. Medicinal Crop. Sci. 2004;12(3): 237-242
  16. Hovius, R., H. Lambrechts, K. Nocolay and B. de Kruijff. Improved methods to isolate and subfractionate rat liver mitochondria. Lipid composition of the inner and outer membrane. Biochim. Biophys. Acta. 1990;1021:217-226 https://doi.org/10.1016/0005-2736(90)90036-N
  17. Re, R., N. Pellegrini, A. Proteggente, A. Pannala, M. Yang and C. Rice-Evans. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 1999;26 :1231-1237 https://doi.org/10.1016/S0891-5849(98)00315-3
  18. Erel, O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin. Biochem. 2004;37:277-285 https://doi.org/10.1016/j.clinbiochem.2003.11.015
  19. Huang, D., B. Ou, M. Hampsch-Woodill, J. A. Flanagan and R. L. Prior. High-throughput assay of oxygen radical absorbance capacity(ORAC) using a multichannel liquid handling system coupled with a microplate fluorescence reader in 96-well format. J. Agric. Food Chem. 2002;50:4437-4444 https://doi.org/10.1021/jf0201529
  20. Singleton, V.L. and R. Orthofer. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol. 1999;299:152-178 https://doi.org/10.1016/S0076-6879(99)99017-1
  21. Malterud, K.E., T.L. Farbrot, A.E. Huse and R.B. Sund. Antioxidant and radical scavenging effects of anthraquinones and anthrones. Pharmacology 1993;47:77-85 https://doi.org/10.1159/000139846
  22. Stacey, N.H. and C.D. Klaassen. Inhibition of lipid peroxidation without prevention of cellular injury in isolated rat hepatocytes. Toxicol. Appl. Pharm. 1981;58:8-18 https://doi.org/10.1016/0041-008X(81)90110-1
  23. LeBel, C.P., H. Ischiropoulos and S.C. Bondy. Evaluation of the probe 2',7'-dichlorofluorescin as an indicator of reative oxyten species formation and oxidative stress. Chem. Res. Toxicol. 1992;5:227-231 https://doi.org/10.1021/tx00026a012
  24. Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall. Protein measurements with the Folin phenol reagent. J. Biol. Chem. 1951;193:265-275
  25. Devy, C. and Gautier, R. New perspectives on the biochemistry of superoxide anion and the efficiency of superoxide dismutase. Biochem. Pharmacol. 1990;39:399-405 https://doi.org/10.1016/0006-2952(90)90043-K
  26. Kuramoto, T. Development and application of food materials from plant extracts such as SOD. Up to date Food Processing. 1992;27:22-23
  27. Rorald, W. Naturally occurring nitrite in food. J. Japan Soc. Food Agric. 1975;26:1735-1742 https://doi.org/10.1002/jsfa.2740261116
  28. Jeon, HK., Kim, SC., Jung, NP. Effects of ginseng sponin fraction and cyclophosphamide on the tumoricidal activity of mouse macrophage and antitumor effect. Korean J. Ginseng Sci. 1991;15:99-105
  29. Kim, MJ., Jung, NP. The effects of ginseng saponin on the mouse immune system. Korean J. Ginseng Sci. 1987;11:130-135
  30. Kang, SY., Kim, ND. The anti-hypersensitive effect of red ginseng saponinand the endotheliumderived vascular relaxation. Korean J. Ginseng Sci. 1992;16:175-182
  31. Joo, CN., Kim, JH. Study on the hypoglycemic action of ginseng saponin on streptozotocin induced diabetic rats. Korean J. Ginseng Sci. 1991;16:190-197
  32. Oliveira, ACC., Perez, AC., Merino G., Prietp, JG., Alvarez, AI. Protective effects of Panax ginseng on muscle injury and inflammation after eccentric exercise. Comparative Biochemistry and Physiology Part C. 2001;130:369-377
  33. Kim, JS., Kim, KW., Choi, KJ., Kwak, YK., Im, KS., Lee, KM., Chung, HY. Screening of anti-oxidative components from red ginseng saponin. Korean J. Ginseng Sci. 1996;20:173-178
  34. Ryu, GH. Present status of red ginseng products and its manufacturing process. Food Industry and Nutrition. 2003;8:38-42
  35. Kitagawa, I. Chemical studies on crude drug processing. I. On constituents of ginseng radix rubra(1). Yakugaku Zasshi 1983;103:612-622
  36. Kim, KH., Lee, YS., Jung, IS., Park, SY., Chung, HY., Lee, IR., Yun, YS. Acidic polysaccharide from Panax ginseng, ginsan, induces Th1 cell and macrophage cytokines and generates LAK cells in synergy withr II-2. Planta media. 1998;64:110-115 https://doi.org/10.1055/s-2006-957385
  37. Lee, JW., Sohn, HO., Do, JH. Function of the water soluble browning reaction products isolated from Korean red ginseng 2. Linoleic acid, Oxbrain autoxidant and $Fe^{2+}$ ADP/NAD system. Korean J. Ginseng Res. 2000;24:35-40
  38. 김성진, 신순식, 서부일, 지선영. 산삼, 장뇌삼, 인삼의 항암효과에 대한 연구. 대한본초학회지. 2004;19(2):41-50
  39. 單書健, 神農本草經校證, 吉林科學技術出版社. 1988;150
  40. Yoshizawa, S., Horiuchi, T., Yoshida, T., Okuda, T. Antitumor promoting activity of (-)-epigallocatechin gallate, the main constitutent of tannin in green tea. Phytother. Res. 1987;1:44-47 https://doi.org/10.1002/ptr.2650010110
  41. Fridovich, I. Biological effects of the superoxide radical. Adv. Enzymol. 1986;58: 62

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