Neuroprotecticve Effect of Sophora Subprostrata on Transient Global Ischemia in Gerbil

광두근(廣豆根)의 Gerbil 전뇌(全腦)허혈에 대한 신경손상방어효능 연구

  • Min, Hong-Kyu (Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Kang, Ho-Chang (Graduate School of East-West Medicine, Kyung Hee University) ;
  • Lee, Hyun-Sam (Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Kim, Sun-Yeou (Graduate School of East-West Medicine, Kyung Hee University) ;
  • Sohn, Young-Joo (College of Oriental Medicine, Sangji University) ;
  • Jung, Hyuk-Sang (Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Sohn, Nak-Won (Graduate School of East-West Medicine, Kyung Hee University) ;
  • Kim, Yoon-Bum (Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University)
  • 민홍규 (경희대학교 한의과대학 한의학연구소) ;
  • 강호창 (경희대학교 동서의학대학원) ;
  • 이현삼 (경희대학교 한의과대학 한의학연구소) ;
  • 김선여 (경희대학교 동서의학대학원) ;
  • 손영주 (상지대학교 한의과대학) ;
  • 정혁상 (경희대학교 한의과대학 한의학연구소) ;
  • 손낙원 (경희대학교 동서의학대학원) ;
  • 김윤범 (경희대학교 한의과대학 한의학연구소)
  • Published : 2008.09.30

Abstract

Objectives : This research was performed to investigate protective effect of Sophora Subprostrata against transient global ischemic damage after 5-min two vessel occlusion. Methods : Gerbils were divided into three groups: Normal group, 5-min two vessel occlusion (2VO) group, Sophora Subprostrata administrated group after 2VO. The CCAs were occluded by microclip for 5min. Sophora Subprostrata was administrated orally(12mg/ml) for 7 days after 2VO. The histological and immunohistochemistrical analysis was performed at 72 hours and 7 days after the surgery each. For histological analysis, the brain tissue was stained with 1% cresyl violet solution and Immunohistochemistry for BAX and Bcl-2 was carried out to examine effect of Sophora Subprostrata on ischemic brain tissue. Results : The results showed that (1) Sophora Subprostrata has the protective effect against ischemia in CA1 area of the gerbil hippocampus 7 days after 5-minute occlusion, (2) the treatment of Sophora Subprostrata inhibits the expression of Bax relatively after 2VO-induced ischemia. That protective effect of the Sophora Subprostrata seems to be performed by regulating the proportion of Bax and Bcl-2 protein, (3) in hypoxia/reperfusion model using PC12 cell, the Sophora Subprostrata extract has the protective effect against ischemia in the dose of $2{\mu}/m{\ell}$ and $20{\mu}/m{\ell}$.This study suggests that Sophora Subprostrata has neuroprotective effect against neuronal damage following cerebral ischemia in vivo with a widely used experimental model of cerebral ischemia in Mongolian gerbils and that Sophora Subprostrata regulates the proportion of Bax and Bcl-2 protein following ischemia. And, Sophora Subprostrata extract has protective effects also on a hypoxia/reperfusion cell culture model using PC12 cell. Conclusions : Sophora Subprostrata has protective effects against ischemic brain damage at the early stage of ischemia.