Antimutagenic and Cytotoxic Effects of an Ethanol Extract of Buckwheat Sprout

메밀싹 에탄올 추출물의 항돌연변이원성 및 암세포 성장억제 효과

  • Cui, Cheng-Bi (Department of Agriculture, Yanbian University) ;
  • Lee, Eui-Yong (R&D Team, Bakery Plant, CJ Corp.) ;
  • Ham, Seung-Shi (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Lee, Deuk-Sik (Department of Foodservice Industry, Hanzhong University)
  • 최승필 (연변대학 농학원 식품과학학부) ;
  • 이의용 (CJ(주) 베이커리공장 기술개발팀) ;
  • 함승시 (강원대학교 바이오산업공학부) ;
  • 이득식 (한중대학교 외식산업학과)
  • Published : 2008.09.30

Abstract

This study was performed to determine the antimutagenic and anticancer effects of ethanol extract of buckwheat sprout using Ames test and SRB assay, respectively. An ethyl acetate fraction (200 ${\mu}/plate$) from the ethanol extract of buckwheat sprout showed inhibition rate of 80.6% against the mutagenesis induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) in Salmonella typhimurium TA100 strain. Also the ethyl acetate fraction (200 ${\mu}/plate$) showed higher antimutagenic activity than other fractions against the mutagenesis induced by 4-nitroquinoline-1-oxide (4NQO) in Salmonella typhimurium TA98 and TA100. In addition, the ethyl acetate fraction (200 ${\mu}/plate$) showed high antimutagenic effect of 80.9% and 85.9% against the mutation of TA98 and TA100 strains induced by 3-amino-1,4-dimethyl-5H-pyrido-(4,3-b)indol (Trp-P-1), respectively. The cytotoxic effects of each solvent fraction from the ethanol extract of buckwheat sprout against human cancer cell lines including lung carcinoma (A549), gastric carcinoma (AGS), breast adenocarcinoma (MCF-7), hepatocellular carcinoma (Hep3B), and colon adenocarcinoma (Colo 205) were investigated. The ethyl acetate fraction of buckwheat sprout ethanol extract at the concentration of 1.0 mg/ml showed strong cytotoxic activities of 70.3, 94.8, 79.6, 82.3, and 73.2% against A549, AGS, MCF-7, Hep3B and Colo 205 cancer cell lines, respectively.

References

  1. Choi, B. H., Kim, S. K., Park, K. Y. and Park, R. Y. (1995) Agronomic character and productivity of genetic resources in buckwheat (Fagopyrum esculentum Moench) (in Korean). RDA, J. Agric. Sci. 37, 221-227
  2. Shibatr, S., Imal, T., Ghikubu, S. and Miyyahara, T. (1979) The composition of buckwheat flour of various varieties and cultivated at various periods. Rept. Nalt. Food Res.Inst. 34, 1-7
  3. Lee, J.S., Lee, M.H., Chang, Y.K., Ju, J.S. and Son, H.S. (1995) Effects of buckwheat diet on serum glucose and lipid metabolism in NIDDM. Korean J. Nutr. 28, 809-816
  4. Martin, A. and Martin, C. (1997) Comparision of 5 microplate colorimetric assay for in vitro cytotoxicity testing and cell proliferation assays. Cytotechnology 11, 49-54 https://doi.org/10.1007/BF00749057
  5. Kim, S.H., Lee, E.Y. and Ham, S.S. (2007) Antioxidation and antigenotoxic effects of buckwheat sprout extracts. J. Korean Soc. Food Nutr. 36, 955-959 https://doi.org/10.3746/jkfn.2007.36.8.955
  6. Lee, J.S., Soo, S.S., Maeng, Y.S., Chang, Y.K. and Ju, J.S. (1994) Effects of buckwheat on organ on weight, glucose and lipid metabolism in streptozotocin-induced diabetic rats. Korean J. Nutr. 27, 819-827
  7. Ham, S.S., Choi, G.P., Choi, Y.S. and Lee, S.Y. (1994) Antimutagenic study of buckwheat leaves ethanol extract. J. Korean Soc. Food Sci. 23, 698-703
  8. Ham, S.S., Choi, K.P., Choi, Y.S. and Lee, S.Y. (1994) Studies on antimutagenic and lipotropic action of flavonoids of buckwheats. J. Korean Soc. Food Nutr. 23, 698-703
  9. Hertog, M.G., K romhout, D., Aracanis, C., Blackburn, H., Buzina, E., Fidanza, F., Giampaoli, S., Jansen, A., Menotti, A., Nedeljkovic, S., Pekkarinen, M., Simic, B.S., Toshima, H., Feskens, E.J.M., Hollman, P.C.H. and Katan, M.B. (1995) Flavnoid intake and longterm risk of coronary heart disease and cancer in the countries study. Arch. Intern. Med. 155, 381-386 https://doi.org/10.1001/archinte.155.4.381
  10. Kwak, C.S., Lim, S.J., Kim, S.A., Park, S.C. and Lee, M.S. (2004) Antioxidative and antimutagenicity effects of Korean buckwheat, sorghum, millet and Job's tears. J. Korean Soc. Food Sci. Nutr. 33, 921-929 https://doi.org/10.3746/jkfn.2004.33.6.921
  11. Griffith, J.Q., Couch, J.F. and Lindauer, M.A. (1995) Effect of rutin on increased capillary fragility in man. Proc. Soc. Exp. Bio. Med. 55, 228-229
  12. Ham, S.S., Lee, S.Y., Choi, M. and Hwang, H.J. (1998) Antimutagenicity and cytotoxicity effects of woorimil wheat flour extracts added with wila herb and seaweed powder. J. Korean Soc. Food Sci. Nutr. 27, 1177-1182
  13. Kwon, T.B. (1994) Changes in rutin and fatty acids of buckwheat during germination. J. Food Nutr. 7, 124-127
  14. Maron, D.M. and Ames, B.N. (1983) Revised methods for the Salmonella mutagenicity test. Mutation Res. 113, 173-215 https://doi.org/10.1016/0165-1161(83)90010-9
  15. Matsubara, Y., Kumamoto, H., Lizuka, Y., Murakami, T., Okamoto, K., Miyake, H. and Yokoi, K. (1995) Structure and hypotensive effect of flavonoid glycosides in Citrus ubshiu peelings. Agric. Biol. Chem. 49, 900-905
  16. Yoo, K.H., Kim, S.H., Ham, Y.A., Yoo, S.J., Oh, H.T. and Ham, S.S. (2006) Antimutagenic and cytotoxic effects of Fagopyrum esculentum Moenech noodles extracts. J. Korean Soc. Food Nutr. 35, 1291-1296 https://doi.org/10.3746/jkfn.2006.35.10.1291
  17. He, J., Klag, M.J., Whelton, P.K., Mo, J.P., Chen, J.K., Qian, M.C., Mo, P.S. and He, G.Q. (1995) Oats and buckwheat intakes and cardiovascular disease risk factors in buckwheat intakes and cardiovascular disease risk factors in an ethnic minority of China. Am. J. Clin. Nutr. 61, 366-372 https://doi.org/10.1093/ajcn/61.2.366
  18. Matsuhashi, T. (1984) Composition of buckwheat and buckwheat powder. New Food Industry 26, 54-58
  19. Keli, S.O., Hertog, M.G.K., Freskens, E.J.M. and Kromhout, D. (1996) Dietary flavonoids, antioxidant vitamins, and incidence of stroke: The Zutpen study. Arch. Intern. Med. 154, 637-642
  20. Lee, J.S., Park, S.J., Sung, K.S., Han, C.K., Lee, M.H., Jung, C.W. and Kwon, T.B. (2000) Effects of germinated-buckwheat on blood pressure, plasma glucose and lipid levels of spontaneously hypertensive rats. Korean J. Food Sci. Technol. 32, 206-211
  21. Ikeda, K., Arioka, K., Fujii, S., Kusano, T. and Oku, M. (1984) Effect on buckwheat protein quality of seed germination and changes in trypsin inhibitor content. Cereal Chem. 61, 236-240
  22. Lee, S. Y. (1997) History of buckwheat, Buckwheat 1, 3-6
  23. Lee, M.H., Woo, S.J., Oh, S.K. and Kwon, T.B. (1994) Changes in contents and composition of dietary fiber during buckwheat germination. J. Food Nutr. 7, 274-283
  24. Maeng, Y.S., Park, H.K. and Kwon, T.B. (1990) Analysis of rutin contents in buckwheat foods. Korean. J. Food Sci. Technol. 22, 732-737