Antimutagenic and Cytotoxic Effects of an Ethanol Extract of Buckwheat Sprout

메밀싹 에탄올 추출물의 항돌연변이원성 및 암세포 성장억제 효과

  • Cui, Cheng-Bi (Department of Agriculture, Yanbian University) ;
  • Lee, Eui-Yong (R&D Team, Bakery Plant, CJ Corp.) ;
  • Ham, Seung-Shi (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Lee, Deuk-Sik (Department of Foodservice Industry, Hanzhong University)
  • 최승필 (연변대학 농학원 식품과학학부) ;
  • 이의용 (CJ(주) 베이커리공장 기술개발팀) ;
  • 함승시 (강원대학교 바이오산업공학부) ;
  • 이득식 (한중대학교 외식산업학과)
  • Published : 2008.09.30


This study was performed to determine the antimutagenic and anticancer effects of ethanol extract of buckwheat sprout using Ames test and SRB assay, respectively. An ethyl acetate fraction (200 ${\mu}/plate$) from the ethanol extract of buckwheat sprout showed inhibition rate of 80.6% against the mutagenesis induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) in Salmonella typhimurium TA100 strain. Also the ethyl acetate fraction (200 ${\mu}/plate$) showed higher antimutagenic activity than other fractions against the mutagenesis induced by 4-nitroquinoline-1-oxide (4NQO) in Salmonella typhimurium TA98 and TA100. In addition, the ethyl acetate fraction (200 ${\mu}/plate$) showed high antimutagenic effect of 80.9% and 85.9% against the mutation of TA98 and TA100 strains induced by 3-amino-1,4-dimethyl-5H-pyrido-(4,3-b)indol (Trp-P-1), respectively. The cytotoxic effects of each solvent fraction from the ethanol extract of buckwheat sprout against human cancer cell lines including lung carcinoma (A549), gastric carcinoma (AGS), breast adenocarcinoma (MCF-7), hepatocellular carcinoma (Hep3B), and colon adenocarcinoma (Colo 205) were investigated. The ethyl acetate fraction of buckwheat sprout ethanol extract at the concentration of 1.0 mg/ml showed strong cytotoxic activities of 70.3, 94.8, 79.6, 82.3, and 73.2% against A549, AGS, MCF-7, Hep3B and Colo 205 cancer cell lines, respectively.


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