Effect of Dietary Supplementation of Cu-methionine Chelate and Cu-soy Proteinate on the Performance, Small Intestinal Microflora and Immune Response in Laying Hens

사료내 Cu-methionine Chelate와 Cu-soy Proteinate가 산란계의 생산성, 소장내 미생물 균총 및 면역체계에 미치는 영향

  • Paik, I.K. (Department of Animal Science and Technology, College of Industrial Science, Chung-Ang University) ;
  • Kim, C.H. (Department of Animal Science and Technology, College of Industrial Science, Chung-Ang University) ;
  • Park, K.W. (Department of Animal Science and Technology, College of Industrial Science, Chung-Ang University)
  • 백인기 (중앙대학교 산업과학대학 동물자원과학과) ;
  • 김찬호 (중앙대학교 산업과학대학 동물자원과학과) ;
  • 박광월 (중앙대학교 산업과학대학 동물자원과학과)
  • Published : 2008.09.30


This study was conducted to investigate the effect of dietary supplementation of Cu-methionine chelate(Cu-Met) and Cu-soy proteinate(Cu-SP) on the performance, small intestinal microflora and immune response in laying hens. A total of 960 Hy-line $Brown^{(R)}$ laying hens of 39 wks old were assigned to one of the following 6 dietary treatment: control(C), antibiotic(Avilamycine 6 ppm), Cu-Met 50 and Cu-Met 100(50 and 100 ppm Cu as Cu-methionine chelate), Cu-SP 50 and Cu-SP 100(50 and 100ppm Cu as Cu-soy proteinate). Each treatment was replicated 4 times with forty birds per replication, housed in 2 birds per cages. Forty birds units were arranged according to randomized block design. Feeding trial lasted 6 wks under 16 hours lighting regimen. Hen-day and hen-house egg production of groups treated with Antibiotic and Cu supplements tended to be higher than the control with significant difference (P<0.05) shown between Cu-Me 100 and control. Egg weight was significantly (P<0.05) heavier in antibiotic and Cu-SP treatments than Cu-Met treatments but they were not significantly different from the control. Eggshell strength, egg shell thickness, egg yolk color and Haugh unit were not significantly different among treatments. There were no significant differences in leukocytes and erythrocytes in the chicken blood. But mean corpuscular hemoglobin value(MCH) was significantly (P<0.05) higher in Cu-SP 100 than antibiotic treatment. The concentrations of serum IgG and IgA were not significantly different among treatments. Copper concentration in the liver tended to increase as the level of copper supplementation increased, that of Cu-SP 100 being significantly (P<0.05) higher than those of the control and antibiotic treatment. Concentrations of iron and zinc of the liver were not significantly influenced by treatments. Populations of Cl. perfringens and Lactobacilli in the small intestinal content were significantly (P<0.05) influenced by treatments. Population of Cl. perfringens decreased and that of Lactobacilli increased in the copper supplemented groups. The result of this experiment showed that Cu-Met and Cu-SP are comparable to antibiotic in improving egg production in laying hens. Birds fed diets supplemented with Cu-SP produced heavier eggs than those fed diets with Cu-Met. There were no significant differences in the performances between 50 ppm and 100 ppm copper supplementation as organic forms.


  1. AOAC 1990 Official Method of Analysis (15th Ed.). Association of Official Analytical Chemists. Arlington VA
  2. Aoyagi S, Backer DH 1993 Protective effect of copper-amino acid complexes against inhibitory effects of L-cysteine and ascorbic acid. Poultry Sci 72(Suppll):82.(Abstr)
  3. Baker DH, Odle J, Funk MA, Wieland TM 1991 Bioavailability of copper in cupric oxide, cuprous, and in a copper-lysine complex. Poultry Sci 70:177
  4. Bradley BC, Graber G, Condon RJ, Frabish LJ 1983 Effect of graded levels of dietary copper on copper and iron concentrations in swine tissues. J Anim Sci 56:625
  5. Burnell TW, Cromwell GL, Stahly TS 1988 Cited by J. Gohl in Bottom Line of Nutrition Feedstuff. June 13. pp16-18
  6. Cromwell GL, Stahly TS, Mongue HJ 1989 Effect of source and level of copper on performance and liver copper stores in weanling pig. J Anim Sci 67:2996-3002
  7. Dove CR, Haydon KD 1991 The effect of copper addition to diet with various iron levels on the performance and hematology of weanling swine. J Anim Sci 69:2013-2019
  8. Edmonds JS, Izquiendo OA, Baker DH 1985 Feed additive studies with newly weaned pigs: Efficacy of supplemental copper, antibiotics and organic acids. J Anim Sci 60:462
  9. Eisen EJ, Bohren BB, McKean HE 1962 The Haugh unit as a measure of egg albumen quality. Poultry Sci 41:1461-1468
  10. Fisher C, Lauren-Jones AP, Hill KJ, Hardy WS 1973 The effect of copper sulfate on performance and the structure of gizzard in broilers. Br Poult Sci 14:55-68
  11. Jackson N 1977 The effect of dietary copper sulphate on laying performance, nutrient intake and tissue copper and iron levels of the mature, laying, domestic fowl. Br J Nutr 38:93-100
  12. Klasing CK 1998 Minerals. pp234-276 in Comparative Avian Nutrition. CAB International. New York. USA
  13. Lim HS, Paik IK 2003 Effect of supplementary mineral methionine chelates (Zn, Cu, Mn) on the performance and eggshell quality of laying hens. Asian-Aust J Anim Sci 16(12):1804-1808
  14. Lim HS, Paik IK 2006 Effects of dietary supplementation of copper chelates in the form of methionine, chitosan and yeast in laying hens. Asian-Aust J Anim Sci 19(8):1174-1178
  15. Melvin JS 1984 Physiological Properties & Cellular & Chemical Constituents of Blood. Dukes' Physiology of Domestic Animals. 10th Ed
  16. NRC 1998 Nutrient Requirements of Poultry. National Research Council. National Academy of Science. Washington, DC
  17. Paik IK, Seo SH, Um JS, Chang MB, Lee BH 1999 Effect of supplementary copper-chelate on the performance and cholesterol level in plasma and breast muscle of broiler chickens. Asian-Aust J Anim Sci 12(5):794-798
  18. Paik IK 2001a Management of excretion of phosphorus, nitrogen and pharmacological level minerals to reduce enviornmental pollution from animal production. Asian-Aust J Anim Sci 14 (3):384-394
  19. Paik IK 2001b Application of chelated minerals in animal production. Asian-Aust J Anim Sci 14, Special Issue:191-198
  20. Pearce J, Jackson N, Stevenson MH 1983 The effect of dietary intake and of the dietary concentration of copper sulphate on the laying domestic fowl: Effect on some aspects of lipid, carbohydrate and amino acid metabolism. Br Poult Sci 59: 1099
  21. Pesti EG, Bakalli RI 1996 Studies on the feeding of cupric sulfate pentahydrate and cupric citrate to broiler chickens. Poult Sci 75:1086-1091
  22. Roof MD, Mahan DC 1982 Effect of carbadox and various dietary copper level for weanling swine. J Anim Sci 55: 1109
  23. SAS Institute 1996 $SAS/STAT^{\circledR}$ User's Guide Release. 6.12 Edition. SAS Institute Inc. Cary NC. USA
  24. Shurson GC, Ku PK, Waxler GL, Yokoyama MT, Moler ER 1990 Physiological relationships between microbiological status and dietary copper levels in the pig. J Anim Sci 68: 1061
  25. Underwood EJ 1977 Trace Elements in Human and Animal Nutrition. 5th ed. Academic Press Inc., New York
  26. Ward TL, Watkins KL, Southern LL, Hoyt PG, French DD 1991 Interactive effects of sodium zeolite-A and copper in growing swine: Growth and bone and tissue mineral concentration. J Anim Sci 69:726
  27. Wedekind KJ, Hortin AE, Baker DH 1992 Methodology for assessing zinc bioavailability: efficacy estimates for zinc-methionine, zinc sulfate, and zinc oxide. J Anim Sci 70:178-187
  28. Zhou W, Kornegay ET, Lindemann MD, Swinkels JWGM, Welton MK, Wong EA 1994 Stimulation of growth by intravenous injection of copper in weanling pigs. J Anim Sci 72:2395-2403
  29. 김윤기 백인기 1993 구리 공급원과 첨가 수준이 육계의 생산성에 미치는 영향. 한국축산학회지 35(1):52-59
  30. 김병한 임희석 남궁환 백인기 1993 이유자돈 사료 첨가제로서 Copper chelates (메치오닌, 키토산, 효모)의 효과. 동물자원과학회지 45(1):49-56
  31. 민승기 남궁환 백인기 1993 사료첨가제로써 구리복합제가육계의 생산성에 미치는 영향. 한국영양사료학회지 17(5):247-257
  32. 민승기 엄재상 백인기 1994 메치오닌-구리 및 단백질-구리 복합제가 육계와 쥐의 성장과 광물질 대사 및 장내 균총에 미치는 영향. 한국 영양사료학회지 18(2):103-113
  33. 백인기 2000 약리적 수준 사용 광물질의 착염화. 단미. 보조사료 산업발전을 위한 심포지움. 한국단미사료협회. pp 37-60
  34. 백인기 남궁환 최영진 1991 황산동과 지방의 상호작용이 육계의 생산성에 미치는 영향. 한국영양사료학회지 15(6):281-286
  35. 이한규 2005 Fe-soy proteinate를 이용한 철분강화계육 및 계란 생산에 관한 연구. 제103회 석사학위논문, 중앙대학교
  36. 홍성진 임희석 백인기 2002 사료내 Cu 및 Zn-Methionine Chelates 첨가가 육계의 생산성에 미치는 영향. 한국동물자원과학회지 44(4):399-406

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