DOI QR코드

DOI QR Code

On-line Monitoring of a Glucose Concentration on a Fermentation Process of Wine for an Automatic Control of a Fermentation Process

발효공정 자동제어를 위한 포도주 발효 중 포도당 농도 온라인 측정

  • Song, Dae-Bin (Division of Agricultural Engineering, Gyeongsang National University/Institute of Agricultural & Life Science)
  • Published : 2008.08.25

Abstract

A flow injection analysis method (FIA), which analyzes sample conditions after injecting a sample and reagents into a continuous stream, are recognized as the most adequate analyzing method according to the increase of sampling frequency, the decrease of measuring time and the diversity of measuring targets. Specially, the FIA is considered to be used effectively for the control of a fermentation process to produce fermentation food and useful microbial production by activation of a fermentation industry for development of biological materials. In this study, a flow injection analysis sensor unit was developed for on-line monitoring of the fermentation process. The performance was verified by on-line measuring the concentration of glucose of the fermentation process of wine. The glucose concentrations of the samples were measured every 12 hours during the whole fermentation process and compared with those by a HPLC. The concentration relative errors of glucose on the fermentation process of wine showed below 30% within 72 hours and over 50% after the 72 hours. The sensor unit had potential to on-line monitoring of the fermentation process but some problems to overcome for an commercial application.

References

  1. Kim, H. K. and B. S. Noh. 2002. Optimization of Sikhe processing using the obtained data by biosensor. Korean J. Food Sci. Technol. 34(1):65-72. (In Korean)
  2. Kim, J. H., D. H. Rhie, T. J. Kim and B. S. Noh. 1998. Development of biosensor for simultaneous determination of glucose, lactic acid and ethanol. Korean J. Food Sci. Technol. 30(1):22-34. (In Korean)
  3. Park, I. S., J. H. Kim, T. J. Kim, N. S. Kim and B. S. Noh. 1996. Simultaneous determination of glucose and ethanol of Takju by biosensor using dual cathode electrode. Korean J. Food Sci. Technol. 28(5):974-980. (In Korean)
  4. Rajendran, V. and J. Irudayaraj. 2002. Detection of glucose and lactose in milk with a microdialysis-coupled flow injection amperometric sensor. J. Dairy Sci. 85:1357-1361 https://doi.org/10.3168/jds.S0022-0302(02)74201-X
  5. Rhee, J. I. 1998. On-line monitoring of glucose and acetate by flow-injection analysis in Escherichia coli fermentation process. Korean J. Biotechnol. Bioeng. 13(3):244-250. (In Korean)
  6. Rocha, I. and E. C. Ferreira. 2002. On-line simultaneous monitoring of glucose and acetate with FIA during high cell density fermentation of recombinant E. coli. Analytica Chimica Acta. 462:293-304 https://doi.org/10.1016/S0003-2670(02)00347-1
  7. Ruzicka, J. and E. H. Hansen. 1975. Flow injection analysis. Part I. A new concept of fast continuous flow analysis. Analytica Chimica Acta. 78:145 https://doi.org/10.1016/S0003-2670(01)84761-9
  8. Schugerl, K., B. Hitzmann, H. jurgens, T. Kullick, R. Ulber and B. Weigal. 1996. Challenges in integrating biosensors and FIA for on-line monitoring and control. TIBTECH. 14:21-31 https://doi.org/10.1016/0167-7799(96)80910-3
  9. Song, D. B., H. S. Jung, S. K. Lee, D. H. Jung and S. W. Park. 2007. Performance evaluation of an auto sampling and filtering unit of substrate solution using a diaphragm pump. J. of Biosystems Engineering 32(4):263-268. (In Korean) https://doi.org/10.5307/JBE.2007.32.4.263
  10. Song, D. B. and J. Irudayaraj. 2003. Measurement of glucose concentration using $\mu$FIA biosensor J. of Biosystems Engineering. 28(5):465-468. (In Korean) https://doi.org/10.5307/JBE.2003.28.5.465
  11. Werasak, S., W. Supinda, S. Pornpimol, T. Morakot and K. Krissanapong. 1999. Dual electrode signal-substracted biosensor for simultaneous flow injection determination of sucrose and glucose. Analytica Chimica Acta. 380:7-15 https://doi.org/10.1016/S0003-2670(98)00710-7
  12. White, S. F., I. E. Tothill, J. D. Newman and A. P. F. Turner. 1996. Development of a mass-producible glucose biosensor and flow-injection analysis system suitable for on-line monitoring during fermentation. Analytica Chimica Acta. 321:165-172 https://doi.org/10.1016/0003-2670(95)00514-5