Effect of CIMT on the Functional Improvement and BDNF Expression in Hemiplegic Rats Whose Somatomotor Area was Removed

체성운동영역이 제거된 편마비 흰쥐에서 억제 유도치료가 기능향상과 BDNF 발현에 미치는 효과

  • Published : 2008.09.28


CIMT(Constraint Induced Movement Therapy) is to improve the function and use of damaged upper limbs by not only confinement of unaffected limbs' exercise but also inducement of affected limbs' one. The purpose of the study is to verify the effect of CIMT by means of motor behaviour test and immunohistochemistry, using animal models. This study was analyzed using 40 male Sprague-Dawley rats as the experimental groups and 40 ones as the control groups. The rats were divided into two random groups : one group as an experimental group which was operated on under anesthesia and removed somatomotor regions with CIMT and the other as the control group without CIMT.Postural Reflex Test, Beam Walking Test, Limb Placement Test and Immunohistochemistry were run on the day 1, 3 , 7 and day 14 following surgery to each 10 rat. As a result, this study demonstrates that CIMT might be an effect method to verify the plasticity of central nervous system as motor behaviour test made all high scores (p<.05) and BDNF was high too in experimental groups.

억제유도치료는 건측 상지의 운동을 제한하고 환측 상지의 운동을 유도함으로써 손상된 상지의 기능 및 사용을 향상시키는 치료이다. 본 연구의 목적은 동물모델을 이용하여 억제유도치료의 효과를 운동행동검사와 면역조직 화학법으로 검증하기 위함이다. 본 연구에서는 Sprague-Dawley계 흰쥐 수컷 실험군 40마리 대조군 40마리를 사용하였다. 실험동물은 마취 후 체성운동영역을 제거하여 억제유도치료를 적용한 실험군과 적용하지 않은 대조군으로 나누었다. 실험동물은 수술 후 1일, 3일, 7일, 14일째 10마리씩 나누어 자세 반사 검사, 막대 걷기 검사, 앞다리 배치 검사, 면역조직 화학반응을 실시하였다. 결과는 실험군의 운동행동 검사에서 모두 유의하게 높았고(p<.05), BDNF 발현은 시간이 지날수록 실험군의 발현량이 높게 나타났다. 이와 같은 결과는 중추신경계의 가소성을 검증 하는데 기여하리라 생각된다.


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