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Effects of Salinity and Standard Toxic Metals (Cu, Cd) on Fertilization and Embryo Development Rates in the Sea Urchin (Strongylocentrotus nudus)

염분과 표준 독성물질(Cu, Cd)이 둥근성게(Strongylocentrotus nudus)의 수정 및 배 발생률에 미치는 영향

Hwang, Un-Ki;Lee, Chung-Won;Lee, Seung-Min;An, Kyoung-Ho;Park, Seung-Youn
황운기;이충원;이승민;안경호;박승윤

  • Published : 2008.07.31

Abstract

Effects of salinity and standard toxic metals on fertilization and embryo development rates were investigated in the sea urchin, Strongylocentrotus nudus. Spawning was induced by injecting 1ml of 0.5 M KCl into the coelomic cavity. The fertilization and embryo development rates were below 20% when salinity was 25 psu or lower, but were above 90% when salinity was between 30 and 35 psu. The fertilization and embryo development rates in the control condition (not including Cu and Cd) were greater than 90%, but decreased with a high negative correlation (r) of 0.89 and 0.91 with the increasing of Cu and Cd concentrations, respectively. These results suggest that salinity concentrations for successful fertilization and normal embryogenesis of S. nudus are between 30 and 35 psu, and the biological assays of fertilization and embryo development rates using S. nudus are useful methods for the ecological toxicity test of marine pollution elements.

Keywords

Strongylocentrotus nudus;Fertilization rate;Embryogenesis;Salinity;Toxicity

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