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Inhibition Effect of 3-(4'-hydroxyl-3',5'-dimethoxyphenyl)Propionic Acid in Kimchi with Anti-Atherogenic Activity on the Accumulation of Lipids in the Organs of ApoE(-/-) Mice

ApoE(-/-) Mice에서 배추김치 활성 성분인 3-(4'-hydroxyl-3',5'-dimethoxylphenyl)Propionic Acid의 장기 지질저하 효과

  • Kim, Hye-Yeon (Dept. of Food Science & Nutrition, and Kimchi Research Institute, Pusan National University) ;
  • Noh, Jeong-Sook (Dept. of Food Science & Nutrition, and Kimchi Research Institute, Pusan National University) ;
  • Song, Yeong-Ok (Dept. of Food Science & Nutrition, and Kimchi Research Institute, Pusan National University)
  • 김혜연 (부산대학교 생활환경대학 식품영양학과 및 김치연구소) ;
  • 노정숙 (부산대학교 생활환경대학 식품영양학과 및 김치연구소) ;
  • 송영옥 (부산대학교 생활환경대학 식품영양학과 및 김치연구소)
  • Published : 2008.11.28

Abstract

Preventive effects of 3-(4'-hydroxyl-3',5'-dimethoxyphenyl)propionic acid (HDMPPA), an active compound in Korean cabbage kimchi with anti-atherogenic effects, on the accumulation of lipids in the vital organs of $apoE^{(-/-)}$ mice fed atherogenic diet (AD) were studied. Each group of 10 mice was fed AD for 8 weeks with intraperitoneal injection of either HDMPPA (1 mg HDMPPA/100 g BW/day) or phosphate buffered saline as a vehicle. The organs used for this study were liver, kidney, spleen, lung, testis, and brain. Total cholesterol (TC) concentration of lung was the highest followed by spleen and brain. TC level for the liver was the lowest. In contrast to the results of TC, triglyceride (TG) concentration in the liver was the highest followed by kidney and testis. $ApoE^{(-/-)}$ mice did not have any problem uptaking chylomicron remnant by the liver which carries an extra TG after delivering it to the adipose tissue. HDMPPA retarded TC and TG accumulations in the vital organs. Thiobarbituric acid reactive substances (TBARS) in the brain and spleen were the highest and that in the testis were the lowest. Poly-unsaturated fatty acids in the brain and activated peroxisome in the spleen might be responsible for high TBARS levels in these organs. The greatest antioxidant effect of HDMPPA against lipid peroxidation was observed in the spleen, showing 21.47% decrease. The most noticeable effect of HDMPPA was observed in glutathione (GSH) level. GSH levels of six organs in the HDMPPA group were significantly higher than those of the control group. GSH-peroxidase activity was negatively related to GSH level of the organs except liver and spleen. In conclusion, HDMPPA from Korean cabbage kimchi inhibits the lipid accumulation as well as increases the antioxidant status in the vital organs of $apoE^{(-/-)}$ mice fed an atherogenic diet.

References

  1. Kwon JY, Cheigh HS, Song YO. 2004. Weight reduction and lipid lowering effects of kimchi lactic acid powder in rats fed high fat diets. Korean J Food Sci Technol 36: 1014-1019
  2. Choi SH, Kim HJ, Kwon MJ, Baek YH, Song YO. 2001. The effect of kimchi pill supplementation on plasma lipid concentration in healthy people. J Korean Soc Food Sci Nutr 30: 913-920
  3. Sheo HJ, Seo YS. 2004. The effects of dietary chinese cabbage kimchi juice on the lipid metabolism and body weight gain in rats fed high-calories-diet. J Korean Soc Food Sci Nutr 33: 91-100 https://doi.org/10.3746/jkfn.2004.33.1.091
  4. Kwon MJ, Song YO, Song YS. 1997. Effects of kimchi on tissue and fecal lipid composition and apolipoprotein and thyroxine levels in rats. J Korean Soc Food Sci Nutr 26: 507-513
  5. Kwon MJ, Chun JH, Song YS, Song YO. 1999. Daily kimchi consumption and its hypolipidemic effect in middle-aged men. J Korean Soc Food Sci Nutr 28: 1144-1150
  6. Kwon MJ, Song YS, Choi MS, Park SJ, Jeong KS, Song YO. 2003. Cholesteryl ester transfer protein activity and atherogenic parameters in rabbit supplemented with cholesterol and garlic powder. Life Science 72: 2953-2964 https://doi.org/10.1016/S0024-3205(03)00234-0
  7. Kwon MJ, Song YS, Choi MS, Song YO. 2003. Red pepper attenuates cholesteryl ester transfer protein activity and atherosclerosis in cholesterol-fed rabbits. Clinica Chimica Acta 332: 37-44 https://doi.org/10.1016/S0009-8981(03)00118-9
  8. Kim HJ, Lee JS, Chung HY, Song SH, Suh H, Noh JS, Song YO. 2007. 3-(4'-hydroxyl-3',5'-dimethoxyphenyl)propionic acid, an active principle in Kimchi, inhibit development of atherosclerosis in rabbits. J Agric Food Chem 55: 10486-10492 https://doi.org/10.1021/jf072454m
  9. Noh JS. 2008. Protective mechanism of 3-(4'-hydroxyl-3',5'-dimethoxyphenyl) propionic acid on the progression of atherosclerosis under apoE knockout mice. PhD Dissertation. Pusan National University
  10. Cheigh HS, Yu R, Choi HJ, Jun HK. 1996. Biosynthesis of L-ascorbic acid by microorganism in kimchi fermentation process. J Food Sci Nutr 1: 37-40
  11. Lee YO, Cheigh HS. 1996. Antioxidant activity of various solvent extracts from freeze dried kimchi. Korean J Life Sci 6: 66-71
  12. Cheigh HS, Hwang JH. 2000. Antioxidative characteristics of kimchi. Food Ind Nutr 5: 52-56
  13. Park KY, Kim HC, Jung KO. 2000. Anticarcinogenic effect of kimchi extract on 2-AAF-induced rat hepatocarcinogenesis system. J Korean Assoc Cancer Prev 5: 81-86
  14. Shin KS, Chae OW, Park IC, Hong SI, Choe TB. 1998. Antitumor effects of mice fed with cell lysate of lactobacillus plantarum isolated from kimchi. Korean J Biotechnol Bioeng 13: 357-363
  15. Cho EJ, Choi JS, Kim SH, Park KY, Rhee SH. 2004. In vitro anticancer effect of active compounds from chinese cabbage kimchi. J Korean Assoc Cancer Prev 9: 98-103
  16. Kim JH, Ryu JD, Lee HG, Park JH, Moon GS, Cheigh HS, Song YO. 2002. The effect of kimchi on production of free radicals and anti-oxidative enzyme activities in the brain of SAM. J Korean Soc Food Sci Nutr 31: 117-123 https://doi.org/10.3746/jkfn.2002.31.1.117
  17. Fujiwara M, Itokawa Y, Uchino H, Inoue K. 1972. Anti-hypercholesterolemic effect of sulfur containing amino acid, S-methyl-L-cysteine sulfoxide isolated from cabbage. Experientia 28: 254-255. https://doi.org/10.1007/BF01928671
  18. Kawada T, Hagigara K, Iwai K. 1986. Effect of capsaicin on lipid metabolism in rats fed diet. J Nutr 116: 1272-1281 https://doi.org/10.1093/jn/116.7.1272
  19. Gonen A, Harats D, Rabinkov A, Miron T, Mirelman D, Wilchek M, Weiner L, Ulman E, Levkovitz H, Ben-Shushan D, Shaish A. 2005. The antiatherogenic effect of allicin: possible mode of action. Pathobiology 72: 325-334 https://doi.org/10.1159/000091330
  20. Giri J, Devi TK, Meerernani S. 1984. Effect of ginger on serum cholesterol levels. Ind J Nutr Dietet 21: 433-436
  21. Lee YM, Kwon MJ, Kim JK, Suh HS, Choi JS, Song YO. 2004. Isolation and identification of active principle in Chinese cabbage Kimchi responsible for antioxidant effect. Korean J Food Sci Technol 36: 129-133
  22. Moon SJ. 1996. Nutrition problems of Korean. Korean J Nutr 29: 371-380
  23. AHA report. 2008 Heart and Stroke Statistical Update. Available from: http://www.americanheart.org/presenter.jhtml?identifier=3053235 (accessed Aug. 01. 2008)
  24. McGrandy RB, Hegsted DM, Stare FJ. 1967. Dietary fats, carbohydrates and atherosclerotic vascular disease. New Engl J Med 277: 186-192 https://doi.org/10.1056/NEJM196707272770405
  25. Simopoulos AP. 1991. Omega-3 fatty acids in health and disease and in growth and development. Am J Clin Nutr 54: 438-463 https://doi.org/10.1093/ajcn/54.3.438
  26. Sung NJ, Lee SJ, Shin JH, Chung MJ, Lim SS. 1998. Effects of Houttuynia cordata Thunb powder and juice on lipid composition of liver, brain and kidney in dietary hypercholesterolemic rats. J Korean Soc Food Sci Nutr 27: 1230-1235
  27. Kim YE, Kwon EK, Oh SW, Han DS, Kim IH, Lee CH, Lee HJ. 2005. The effects of dietary supplements included green tea, buckwheat and grape leaf extract on lipid metabolism and on regression of fatty streak lesions in F1B golden syrian hamsters fed the atherogenic diet. Korean J Food Sci Technol 37: 962-969
  28. Bocksch L, Stephens T, Lucas A, Singh B. 2001. Apolipoprotein E: possible therapeutic target for atherosclerosis. Curr Drug Targets Cardiovasc Haematol Disord 1: 93-16 https://doi.org/10.2174/1568006013337944
  29. Folch IL, Stanley GH. 1956. A simple method for the isolation and purification of total lipids from animal tissue. J Biochem 223: 498-499
  30. Ellman GL. 1959. Tissue sulfhydryl group. Arch Biochem Biophys 82: 70-72 https://doi.org/10.1016/0003-9861(59)90090-6
  31. Bradford MM. 1976. A rapid and sensitive method for the quantification of microgram quantities of proteins utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254 https://doi.org/10.1016/0003-2697(76)90527-3
  32. Flohe L, Gunzler WA. 1984. Assays of glutathione peroxidase. Methods Enzymol 105: 114-121 https://doi.org/10.1016/S0076-6879(84)05015-1
  33. Mihara M, Uchiyama M. 1978. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem 86: 271-278 https://doi.org/10.1016/0003-2697(78)90342-1
  34. Ko YR. 2004. Antioxidant and lipid lowering effects of ethanol extracts of bamboo. MS Thesis. Pusan National University
  35. Myung SJ, Kim YS, Kim SY, Nam SW, Shim KN, Kim HJ, Kim JS, Seo DW, Lee SK, Kim MH, Min YI, Choi HS, Kim KS. 1998. Effect of cholesterol rich diet on the formation of cholesterol gallstones in hamster. Korean Soc Gastroenterol 32: 75-86
  36. Kim HS, Kim GJ, Kim HS. 1998. Effect of the feeding Platycodon frandiflorum on lipid components of liver and liver function in hypercholesterolemia rats. Korean J Food Nutr 11: 312-318
  37. Gruffat D, Durand D, Graulet B, Bauchart D. 1996. Regulation of VLDL synthesis and secretion in the liver. Reprod Nutr Dev 36: 375-389 https://doi.org/10.1051/rnd:19960404
  38. Jeon HN. 2000. Effect of kimchi solvent fraction on lipid profile in organs of rabbit fed cholesterol diet. MS Thesis. Pusan National University
  39. Koh JB, Choi MA. 2001. Effect of Cordyceps militaris on lipid metabolism in rats fed cholesterol diet. Kor J Nutr 34: 265-270
  40. van Herpen NA, Schrauwen-Hinderling VB. 2008. Lipid accumulation in non-adipose tissue and lipotoxicity. Physiol Behav 94: 231-241 https://doi.org/10.1016/j.physbeh.2007.11.049
  41. Yobimoto K, Matsumoto K, Huong N, Kasai R, Yamasaki K, Watanabe H. 2000. Suppressive effects of vietnamese ginseng saponin and its major component majonoside-R2 on psychological stress-induced enhancement of lipid peroxidation in the mouse brain. Pharmacol Biochem Behavior 66: 661-665 https://doi.org/10.1016/S0091-3057(00)00257-4
  42. Ryu HS, Kim J, Kim HS. 2006. Enhancing effect of Sorghum bicolor L. Moench (Sorghum, su-su) extracts on mouse spleen and macrophage cell activation. Korean J Food Nutr 19: 176-182
  43. Gavazza M, Catala A. 2001. The effect of alpha-tocopherol on the lipid peroxidation of mitochondria and microsomes obtained from rat liver and testis. Mol Cell Biochem 225: 121-128 https://doi.org/10.1023/A:1012274206337
  44. Bray TM, Kubow S, Bettger WJ. 1986. Effect of dietary zinc on endogenous free radical production in rat lung microsomes. J Nutr 116: 1054-1060 https://doi.org/10.1093/jn/116.6.1054
  45. Kubow S, Bray TM, Bettger WJ. 1986. Effects of dietary zinc and copper on free radical production in rat lung and liver. Can J Physiol Pharmacol 64: 1281-1285 https://doi.org/10.1139/y86-216
  46. Sullivan JF, Jetton MM, Hahn HKJ. 1980. Enhanced lipid peroxidation in liver microsomes of zinc deficient rats. Am J Clin Nutr 33: 51-56 https://doi.org/10.1093/ajcn/33.1.51
  47. Burke JP, Fenton MR. 1985. Effect of a zinc-deficient diet on lipid peroxidation in liver and tumor subcellular membranes. Proc Soc Exp Biol Med 179: 187-191 https://doi.org/10.3181/00379727-179-42083
  48. Hammermueller JD, Bray TM, Bettger WJ. 1987. Effect of zinc and copper deficiency on microsomal NADPH-dependent active oxygen generation in rat lung and liver. J Nutr 117: 894-901 https://doi.org/10.1093/jn/117.5.894
  49. Kim HT, An EN. 2000. The effect of vitamin E supplementation, dietary restriction on T-cell, MDA and antioxidant enzyme of rats. Korean J Physi Edu 39: 592-600
  50. Farooqui MYH, Day WW, Zamorano DM. 1987. Glutathione and lipid peroxidation in the aged rat. Comp Biochem Physiol 88B: 117-180

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