Integral Antioxidative Capacity of Extracts by Pressurized Organic Solvent from Natural Plants in Jeju

제주 자생식물 고압용매 추출물의 통합적 항산화 능력

  • Kim, Mi-Bo (Dept. of Food Bioengineering, Cheju National University) ;
  • Hyun, Sun-Hee (Dept. of Food Bioengineering, Cheju National University) ;
  • Park, Jae-Sung (Dept. of Food Bioengineering, Cheju National University) ;
  • Kang, Mi-Ae (Dept. of Food Bioengineering, Cheju National University) ;
  • Ko, Young-Hwan (Dept. of Food Bioengineering, Cheju National University) ;
  • Lim, Sang-Bin (Dept. of Food Bioengineering, Cheju National University)
  • 김미보 (제주대학교 식품생명공학과) ;
  • 현선희 (제주대학교 식품생명공학과) ;
  • 박재성 (제주대학교 식품생명공학과) ;
  • 강미애 (제주대학교 식품생명공학과) ;
  • 고영환 (제주대학교 식품생명공학과) ;
  • 임상빈 (제주대학교 식품생명공학과)
  • Published : 2008.11.28


Twenty natural plants from Jeju were extracted by pressurized organic solvent (100% methanol, $40^{\circ}C$, 13.6 MPa, 10 min). Extraction yield, total phenolic content (TPC) and integral antioxidant capacity were measured, and each component was identified by GC/MS. Extraction yields were high as 21.8%, 21.5, 21.1, 20.7 and 20.1% in Rhus javanica, Euscaphis japonica, Alnus firma, Sapium japonicum and Sorbus alnifolia, respectively. The extracts containing high TPC (mg GAE/g of dry sample) were obtained from Malus sieboldii (68.3), Sapium japonicum (57.6), Pyrrosia lingua (56.6) and Euscaphis japonica (55.1). Integral antioxidant capacities of water-soluble substances were 598, 394, 293 and $270\;{\mu}mol$ ascorbic acid equivalent/g in Geranium thunbergii, Sapium japonicum, Cornus kousa and Rhus javanica, respectively. Integral antioxidant capacities of lipidsoluble substances were 611, 314, 296 and $242\;{\mu}mol$ trolox equivalent/g in Ardisia crenata, Ostrya japonica, Geranium thunbergii and Quercus acuta, respectively. Fifteen major peaks were identified by GC/MS from the extract of pressurized organic solvent from Sapium japonicum. Two polyphenols (gallic acid (retention time (RT) 19.7 min)) and quercetin (33.5 min)), ascorbic acid (RT 35.3 min), and several fatty acids (retention time 18.6, 21.0, 21.8, 21.9 and 23.6 min) were identified, and gallic acid was the major polyphenol component due to high peak area.


  1. Kim JP. 1998. A study on development of natural antioxidants. Bioind News 11: 6-14
  2. Song JW, Min KJ, Cha CG. 2008. Antioxidative and antitumor activity of extracts from Saussurea lappa. J Env Hlth Sci 34: 55-61
  3. Kim JW, Moon BS, Park YM, Yoo NH, Ryoo IJ, Nguyen TC, Yoo ID, Kim JP. 2005. Structures and antioxidant ac-tivity of diketopiperazines isolated from the mushroom Sarcodon aspratus. J Korean Soc Appl Biol Chem 48: 93-97
  4. Kang MC, Lee JY, Lee JA, Han JH, Kim BS, Kim GO. 2008. Antioxidant effects and melanin inhibitory effect of natural Pimpinella komarovii extracts in Jeju island. Korean J Biotechnol Bioeng 23: 77-82
  5. Huang MT, Ho CT, Lee C. 1992. Phenolic Compounds in Food and Their Effects on Health (II), Antioxidants and Cancer Prevention. ACS Symp Series 507. American Chemical Society, Washington, DC. p 54-71
  6. Mukhopadhyay S, Luthria DL, Robbins RJ. 2006. Optimazation of extraction process for phenolic acids from black cohosh (Cimicifuga racemosa) by pressurized liquid extraction. J Sci Food Agric 86: 156-162
  7. Lee YJ, Shin DH, Chang YS, Kang WS. 1993. Antioxidative effect of Rhus javanica Linne extract by various solvents. Korean J Food Sci Technol 25: 677-682
  8. Peschel W, Sanchez-Rabaneda F, Diekmann W, Plescher A, Gaetzia A, Gartzia I, Jimenez D, Lamuela-Raventos R, Buxaderas S, Codina C. 2006. An industrial approach in the search of natural antioxidants from vegetable and fruit wastes. Food Chem 97: 137-150
  9. Besco E, Braccioli E, Vertuani S, Ziosi P, Brazzo F, Bruni R, Saccetti G, Manfredini S. 2007. The use of photochemiluminescence for the measurement of the integral antioxidant capacity of baobab products. Food Chem 102: 1352-1356
  10. Chiou A, Karathanos VT, Mylona A, Salta FN, Preventi F, Andrikopoulos NK. 2007. Currants (Vitis vinifera L.) content of simple phenolics and antioxidant activity. Food Chem 102: 516-522
  11. Hyun SH, Jung SK, Jwa MK, Song CK, Kim JH, Lim SB. 2007. Screening of antioxidants and cosmeceuticals from natural plant resources in Jeju island. Korean J Food Sci Technol 39: 200-208
  12. Kim IW, Shin DH, Choi U. 1999. Isolation of antioxidative components from the bark of Rhus verniciflua S. screened from some chinese medical plants. Korean J Food Sci Technol 31: 885-863
  13. Giocosa A, Filiberti R. 1996. Free radicals, oxidative damage and degenerative disease. Eur J Cancer Prev 5: 307-312
  14. Ra KS, Suh HJ, Chung SH, Son JY. 1997. Antioxidant activity of solvent extract from onion skin. J Food Sci Technol 29: 595-600
  15. Zhang Y, Dong L, Li J, Chen X. 2008. Studies on the inter-action of gallic acid with human serum albumin in mem-brane mimetic environments. Talanta 76: 246-253
  16. Kim H, Lee SW. 2007. The effects of quercetin on paraquat-induced cell damage. J Korean Soc Emerg Med 18: 41-47
  17. Kim MK. 2001. Formation of superoxide anion in the autoxidation of L-ascorbic acid in the presence of heavy metal ions. Korean J Food Sci Technol 33: 378-383

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