DOI QR코드

DOI QR Code

Discrimination of Hanwoo from Holstein and Mixed Beef by DHPLC

변성 고성능 액체 크로마토그래피를 이용한 한우, 젖소 그리고 혼입육의 구분

  • Ahn, Young-Chang (Department of Chemistry, School of Advanced Science and Basic Science Research Institute, Institute of Tissue and Regeneration Engneering, Dankook University) ;
  • Cho, Min-Ho (Department of Chemistry, School of Advanced Science and Basic Science Research Institute, Institute of Tissue and Regeneration Engneering, Dankook University) ;
  • Seo, Jae-Won (Department of Chemistry, School of Advanced Science and Basic Science Research Institute, Institute of Tissue and Regeneration Engneering, Dankook University) ;
  • Yoon, Il-Kyu (Department of Chemistry, School of Advanced Science and Basic Science Research Institute, Institute of Tissue and Regeneration Engneering, Dankook University) ;
  • Jung, Duck-Hyun (Department of Chemistry, School of Advanced Science and Basic Science Research Institute, Institute of Tissue and Regeneration Engneering, Dankook University) ;
  • Lee, Eun-Young (Department of Chemistry, School of Advanced Science and Basic Science Research Institute, Institute of Tissue and Regeneration Engneering, Dankook University) ;
  • Nam, Youn-Hyoung (Department of Chemistry, School of Advanced Science and Basic Science Research Institute, Institute of Tissue and Regeneration Engneering, Dankook University) ;
  • Park, Su-Min (Department of Chemistry, School of Advanced Science and Basic Science Research Institute, Institute of Tissue and Regeneration Engneering, Dankook University) ;
  • Jang, Won-Cheoul (Department of Chemistry, School of Advanced Science and Basic Science Research Institute, Institute of Tissue and Regeneration Engneering, Dankook University)
  • 안영창 (단국대학교 첨단과학대학 화학과 및 기초과학연구소/조직재생연구소) ;
  • 조민호 (단국대학교 첨단과학대학 화학과 및 기초과학연구소/조직재생연구소) ;
  • 서재원 (단국대학교 첨단과학대학 화학과 및 기초과학연구소/조직재생연구소) ;
  • 윤일규 (단국대학교 첨단과학대학 화학과 및 기초과학연구소/조직재생연구소) ;
  • 정덕현 (단국대학교 첨단과학대학 화학과 및 기초과학연구소/조직재생연구소) ;
  • 이은영 (단국대학교 첨단과학대학 화학과 및 기초과학연구소/조직재생연구소) ;
  • 남윤형 (단국대학교 첨단과학대학 화학과 및 기초과학연구소/조직재생연구소) ;
  • 박수민 (단국대학교 첨단과학대학 화학과 및 기초과학연구소/조직재생연구소) ;
  • 장원철 (단국대학교 첨단과학대학 화학과 및 기초과학연구소/조직재생연구소)
  • Published : 2009.12.20

Abstract

In the meat industry, correct breed information in food labeling is required to assure meat quality. Genetic markers provide corroborating evidence to identify breed. We described the development of DNA markers to discriminate between Korean beef cattle (Hanwoo), Holstein, and mixed cow beefs. As most breeds are standardized for coat colour, the melanocortin 1 receptor (MC1R) gene, involved in the regulation of eu/pheomelanins synthesis, has been suggested as marker for breed traceability of products of animal origin. We also designed sex-determining region Y (SRY) gene specific primers for Y chromosome detection. In this study, fragments of MC1R gene and SRY gene were amplified by multiplex-PCR and subjected to digestion by MspA1I restriction endonuclease. Reaction products were analysised by denaturing high performance liquid chromatography (DHPLC). As a result, we identified 6 DHPLC peak types from MC1R gene and SRY gene analysis. DHPLC method showed more sensitive than RFLP method for DNA fragments analysis. Therefore, DHPLC method can apply to identify for Hanwoo, Holstein and mixed beef.

Keywords

Multiplex-PCR;DHPLC;Korea beef cattle;MC1R

References

  1. Sasazaki, S.; Mutoh, H.; Tsurifune, K.; Mannen, H.; Meat Science. 2007, 77, 161 https://doi.org/10.1016/j.meatsci.2007.02.024
  2. Matukumalli, L. K.; Lawley, C. T.; Schnabel, R. D.; Taylor, J. F.; Allan, M. F.; Heaton, M. P.; O'Connell, J.; Moore, S. S.; Smith, T. P. L.; Sonstegard, T. S.; Tassel, C. P. V.; PLoS ONE. 2009, 4, 5350 https://doi.org/10.1371/journal.pone.0005350
  3. Harding, R. M.; Healy, E.; Ray, A. J.; Ellis, N. S.; Flanagan, N.; Todd, C.; Dixon, C.; Sajantila, A.; Jackson, I. J.; Birch‐Machin, M. A.; Rees, J. L.; Am. J. Hum. Genet. 2000, 66, 1351 https://doi.org/10.1086/302863
  4. Kijas, J. M. H.; Wales, R.; Tornsten, A.; Chardon, P.; Moller, M.; Andersson, L.; Genetics. 1998, 150, 1177
  5. Erdal, M. E.; Barlas, I. O.; Turk. J. Med. Sci. 2000, 30, 501
  6. Lee, S. S.; Yang, Y. H.; Kang, S. Y.; Oh, W. Y.; Yang, B. S.; Ko, S. B.; Oh, S. J.; Kim, K. I.; J. Anim. Sci. & Technol. 2000, 42, 253
  7. Jose, C.; Borro, G.; Berta, L.; Laorden, S.; Cervantes, C. J.; Pigment Cell. 2005, 18, 393
  8. Rolando, A.; Stasio, L. D.; ITAL. J. ANIM. SCI. 2006, 5, 87
  9. Underhill, P. A.; Jin, L.; Lin, A. A.; Genome Res. 1997, 7, 996
  10. Zhang, C. L.; Fowler, M. R.; Scott, N. W.; Lawson, G.; Slater, A.; Food Control. 2007, 18, 1149. https://doi.org/10.1016/j.foodcont.2006.07.018
  11. Dalmassoa, A.; Fontanellab, E.; Piattib, P.; Civeraa, T.; Rosatic, S.; Bottero, M. T.; Molecular and Cellular Probes. 2004, 18, 81 https://doi.org/10.1016/j.mcp.2003.09.006
  12. Pieragostini, E.; Scaloni, A.; Rullo, R.; Luccia, A. D.; Comparative Biochemistry and Physiology Part B. 2000, 127, 1 https://doi.org/10.1016/S0305-0491(00)00218-2
  13. Abbas, A.; Lepelley, M.; Lechevrel, M.; Sichel, F.; J. Biochem. Biophys. Methods. 2004, 59, 121 https://doi.org/10.1016/j.jbbm.2003.12.008
  14. Shiue, Y. L.; Millon, L. V.; Skow, L. C.; Honeycutt, D.; Murray, J. D.; Bowling, A. T.; Chromosome Research. 2000, 8, 45 https://doi.org/10.1023/A:1009275102977
  15. Go, H.; Lee, M.; Li, S.; Kim, K. S.; Kang, S. H.; Journal of the Korean Chemical Society. 2008, 52, 6
  16. Footz, T.; Somerville, M. J.; Tomaszewaki, R.; Sprysak, K. A.; Backhouse, C. J.; GENETIC TESTING. 2003, 7, 4 https://doi.org/10.1089/109065703322783635
  17. Xiao, W.; Oefner, P. J.; HUMAN MUTATION. 2001, 17, 439 https://doi.org/10.1002/humu.1130
  18. Osipova, G. R.; Karmanov, M. E.; Kozlova, S. I.; Evgrafov, O. V.; American Journal of Medical Genetics. 1998, 76, 283 https://doi.org/10.1002/(SICI)1096-8628(19980401)76:4<283::AID-AJMG1>3.0.CO;2-R
  19. Zimmermann, B.; Sheikhah, A. E.; Nicolaides, K.; Holzgreve, W.; Hahn, S.; Clinical Chemistry. 2005, 51, 1598 https://doi.org/10.1373/clinchem.2005.051235
  20. Sivakumaran, T. A.; Kucheria, K.; Oefner, P. J.; CURRENT SCIENCE. 2003, 84, 291
  21. Park, S. B.; Nam, Y. H.; Lee, J. S.; Kang, W.; Jang, W. C.; Journal of the Korean Chemical Society. 2006, 50, 123 https://doi.org/10.5012/jkcs.2006.50.2.123
  22. Lee, G.; Lim, K.; Kim, J. B.; J. Exp. Biomed. Sci. 2007, 13, 71

Cited by

  1. Gold catalysis in total synthesis – recent achievements vol.45, pp.5, 2016, https://doi.org/10.1039/C5CS00721F
  2. Accelerating Spirocyclic Polyketide Synthesis using Flow Chemistry vol.53, pp.19, 2014, https://doi.org/10.1002/anie.201402056
  3. Accelerating Spirocyclic Polyketide Synthesis using Flow Chemistry vol.126, pp.19, 2014, https://doi.org/10.1002/ange.201402056
  4. Gold-Catalyzed Hydrofunctionalizations and Spiroketalizations of Alkynes as Key Steps in Total Synthesis vol.58, pp.5, 2018, https://doi.org/10.1002/ijch.201700056
  5. ChemInform Abstract: Spirodienal, a New Spiroketal from Sorangium cellulosum. vol.40, pp.33, 2009, https://doi.org/10.1002/chin.200933209
  6. A new cytotoxic spiroketal from the myxobacterium Sorangium cellulosum vol.32, pp.6, 2009, https://doi.org/10.1007/s12272-009-1604-4