Effects of Organic Loading Rates on Treatment Performance in a Polyvinylidene Media Based Fixed-Film Bioreactor

Ahmed, Zubair;Oh, Sang-Eun;Kim, In S.

  • Published : 2009.12.31


This study investigated the effects of organic loading rates on simultaneous carbon and nitrogen removal in an innovative fixed-film aerobic bioreactor. The fixed-film bioreactor (FFB) was composed of a two-compartment aeration tank, in which a synthetic filamentous carrier was submerged as biofilm support media, and a settling tank which polyvinylidene media (Saran) was used as settling aid for suspended solids. Three different organic loading rates, ranging from 0.92-2.02 kg chemical oxygen demand/$m^3$/day were applied by varying hydraulic retention time (HRT). The total soluble organic carbon removal efficiencies were in the range of 90-97%. The removal efficiency of ammonia was found to be in the range of 70-84%. Total nitrogen removal efficiency was found to be in the range of 40-45%, which indicates that denitrification reactions occurred simultaneously in the attached biofilm on the fibrous media in the aeration tank. The settling performance of suspended solids was significantly improved due to the presence of Saran media in the settling compartment, even for a short HRT. The fixed-film aerobic bioreactor used in this study demonstrated efficient treatment efficiency even at higher organic loading rates and at short HRTs.


Organic loading rate;Denitrification;Saran media;Fixed-film bioreactor; Settling behaviour


  1. Jou, C. J. G. and Huang, G. C., “A pilot study for oil refinery wastewater treatment using a fixed-film bioreactor,” Adv. Environ. Res., 7(2), 463-469 (2003)
  2. Soddell, J. A. and Seviour, R. J., “Microbiology of foaming in activated-sludge plants,” J. Appl. Bacteriol., 69(2), 145-176 (1990)
  3. Lewandowski, G. and DeFilippi, L. J., Biological treatment of hazardous wastes, John Wiley & Sons, New York (1998)
  4. Dillon, G. R. and Thomas, V. K., “A pilot-scale evaluation of the 444'BIOCARBONE process' for the treatment of settled sewage and for tertiary nitrification of secondary effluent,” Water Sci. Technol., 22(1-2), 305-316 (1990)
  5. Schramm, A., De Beer, D., Gieseke, A., and Amann, R., “Microenvironments and distribution of nitrifying bacteria in a membrane-bound biofilm,” Environ. Microbiol., 2(6), 680-686 (2000)
  6. Sakano, Y., Pickering, K. D., Strom, P. F., and Kerkhof, L. J., “Spatial distribution of total, ammonia-oxidizing, and denitrifying bacteria in biological wastewater treatment reactors for bioregenerative life support,” Appl. Environ. Microbiol., 68(5), 2285-2293 (2002)
  7. Kinner, N. E., Balkwill, D. L., Bishop, P. L., and New Hampshire Univ Durham Dept of Civil Engineering, The microbiology of rotating biological contactor films, Defense Technical Information Center, Ft. Belvoir (1982)
  8. Masuda, S., Watanabe, Y., and Ishiguro, M., “Biofilm properties and simultaneous nitrification and denitrification in aerobic rotating biological contactors,” Water Sci. Technol., 23(7-9), 1355-1363 (1991)
  9. Arvin, E. and Kristensen, G. H., “Phosphate precipitation in biofilms and flocs,” Water Sci. Technol., 15(3-4), 65-85 (1983)
  10. Kim, M. Y., The effect of dissolved oxygen concentration and internal recycle ratio on the removal of nitrogen and organic compounds in the A/O/O biofilm process, MSc. Thesis, Gwangju Institute of Science and Technology, Gwangju (1997)
  11. American Public Health Association, Greenberg, A. E., Eaton, A. D., Clesceri, L. S., American Water Works Association, and Water Environment Federation, Standard methods for the examination of water and wastewater, 18th ed., APHA-AWWA-WEF, Washington, DC (1992)
  12. Canziani, R., Vismara, R., Basilico, D., and Zinni, L., “Nitrogen removal in fixed-bed submerged biofilters without backwashing,” Water Sci. Technol., 40(4-5), 145-152 (1999)
  13. Chui, P. C., Terashima, Y., Tay, J. H., and Ozaki, H., “Performance of a partly aerated biofilter in the removal of nitrogen,” Water Sci. Technol., 34(1-2), 187-194 (1996)
  14. Satoh, H., Yamakawa, T., Kindaichi, T., Ito, T., and Okabe, S., “Community structures and activities of nitrifying and denitrifying bacteria in industrial wastewater-treating biofilms,” Biotechnol. Bioeng., 94(4), 762-772 (2006)
  15. Halling-Sorensen, B. and Hjuler, H., “Simultaneous nitrification and denitrification with an upflow fixed bed reactor applying clinicoptilolite as media,” Water Treat., 7(1), 77-88 (1992)