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The Effect of the Keughachukeo-tang Extract on the Hepatocellular Carcinogenesis and Acute Liver Damage Induced by Diethylnitrosamine and CCl4 in Rats

膈下逐瘀湯이 Diethylnitrosamine과 CCl4로 유발된 흰쥐의 肝癌 形成과 肝損傷에 미치는 영향

  • Heo, Rae-Kyong (Department of Oriental Internal Medicine, College of Oriental Medicine, Won-kwang University) ;
  • Seung, Kee-Moon (Department of Oriental Internal Medicine, College of Oriental Medicine, Won-kwang University) ;
  • Kim, So-Yeon (Department of Oriental Internal Medicine, College of Oriental Medicine, Won-kwang University) ;
  • Je, Jun-tae (Department of Oriental Internal Medicine, College of Oriental Medicine, Won-kwang University) ;
  • Kwon, So-yeon (Department of Oriental Internal Medicine, College of Oriental Medicine, Won-kwang University) ;
  • Moon, Goo (Department of Oriental Internal Medicine, College of Oriental Medicine, Won-kwang University) ;
  • Lee, Jong-Deok (Department of Radiology, Won-kwang University Hospital) ;
  • Won, Jin-Hee (Department of Oriental Internal Medicine, College of Oriental Medicine, Won-kwang University)
  • 허래경 (원광대학교 한의과대학 내과학교실) ;
  • 성기문 (원광대학교 한의과대학 내과학교실) ;
  • 김소연 (원광대학교 한의과대학 내과학교실) ;
  • 제준태 (원광대학교 한의과대학 내과학교실) ;
  • 권소연 (원광대학교 한의과대학 내과학교실) ;
  • 문구 (원광대학교 한의과대학 내과학교실) ;
  • 이종덕 (원광대학교 광주병원 영상의학과) ;
  • 원진희 (원광대학교 한의과대학 내과학교실)
  • Published : 2009.12.30

Abstract

This study was performed to observe the effect of Keughachukeo-tang(KH) extract on the hepatocellular carcinogenesis and acute liver damage induced by Diethylnitrosamine(DENA) and $CCl_4$ in Rats. Experimental groups were divided into four; normal group(Nor), acute liver damage and hepatocellular cancer inducing control group(Con), KH extract 350㎎/㎏/day(KHA), and 700㎎/㎏/day(KHB) administered groups to Con. The results obtained are as follows: The body weight increased in KHA and KHB than Con from 7th week to the 8th week. The activities of Alanine aminotransferase(ALT) were the most increased in the Con among experimental group. The activities of aspartate aminotransferase(AST), alkaline phosphatase(ALP), and lactacte dehydrogenase(LDH) were significantly decreased(p$<$0.05) in the KHA and KHB compared with Con. Alpha fetoprotein(AFP) were the most increased in the Con among experimental groups. The activities of superoxide dismutase(SOD) were the most increased in the Con among experimental groups. The activities of catalase were significantly increased(p$<$0.05) in the KHA and KHB compared with Con. The results of light microscopical observation, a number of hepatocytes were damaged in the Con compared with Nor and KH extract administerd groups. The number of hepatic p53 positive cells was reduced in the KH extract administered groups compared with Con. These results suggest that administration of KH extract suppress or retard on the Hepatocellular Carcinogenesis and acute liver damage induced by DENA and $CCl_4$ in Rats.

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