Investigation of Biological Activities of Enzymatic Hydrolysate of Spirulina

스피루리나 효소가수분해물의 생리활성 탐색

  • 손민희 (호서대학교 식품생물공학과) ;
  • 박근형 (호서대학교 식품생물공학과) ;
  • 최아름 (호서대학교 식품생물공학과) ;
  • 유귀재 (호서대학교 식품생물공학과) ;
  • 인만진 (청운대학교 식품영양학과) ;
  • 김동호 (비타민하우스F&B(주)) ;
  • 채희정 (호서대학교 식품생물공학과)
  • Published : 2009.02.28


Biological activities of enzymatic hydrolysate of spirulina (EHS) were investigated. EHS showed no significant effects on the growth-stimulating activity for lactic-acid bacteria and antioxidant activity. EHS showed slight in vitro growth-inhibitory effects (15% at 1.42 mg/L) on a human cervical cancer cell line (HeLa). In addition, the anticoagulant activities of EHS were measured based on three different pathways: common, intrinsic and extrinsic pathways. As an indication of anticoagulant activity on common pathway, thrombin time (TT) of EHS (100 mg/L) was measured as 155.6 sec. Activated partial thromboplastin time (aPTT) for intrinsic pathway of EHS (1,000 mg/L) was measured as 95.8 sec. Prothrombin time (PT) based on extrinsic pathway of EHS (1,000 mg/L) was measured as 10.6 sec. These data showed that EHS have influences on anticoagulant factors of common pathway and intrinsic pathway. Consequently it was found that EHS could be used as a functional food for blood circulation.


  1. Yang HN, Lee EH, Kim HM. 1997. Spirulina platensis inhibits anaphylactic reaction. Lif Sci 61: 1237-1244
  2. Kay RA. 1991. Microalgae as food and supplement. Crit Rev Food Sci Nutr 30: 555-573
  3. Romana I, Bellitti MR, Nicolaus B, Lama L, Mance C, Pagnotta E, Gambacorta A. 2000. Lipid profile: a useful chemotaxonomic marker for classification of new cyanobacterium in spirulina genus. Phytochem 54: 289-296
  4. Annapurna VV, Deosthale YG, Bamji MS. 1991. Spirulina as a source of vitamin A. Plant Foods Hum Nutr 41: 125-134
  5. Tokusoglu O, Unal MK. 2003. Biomass nutrition profiles of three microalgae: Spirulina platensis, Chlorella vulgaris, and Isochrisis galbana. J Food Sci 68: 1144-1148
  6. Miranda MS, Cintra RG, Barros SBM, Mancini-Filho J. 1998. Antioxidant activity of the microalga Spirulina maxima. Braz J Med Biol Res 31: 1075-1079
  7. Watanabe F, Takenaka S, Kittaka-Katsura H, Ebara S, Miyamoto E. 2002. Characterization and bioavailability of vitamin B12-compounds from edible algae. J Nutr Sci Vitaminol 48: 325-331
  8. Kim HM, Lee EH, Cho HH, Moon YH. 1998. Inhibitory effect of mast cell-mediated immediate-type allergic reactions in rats by spirulina. Biochem Pharmacol 55: 1071-1076
  9. Kim HS, Kim CH, Kim JH, Kwon MC, Cho JH, Gwak HG, Hwang BY, Kim JC, Lee HY. 2006. Comparison of anticancer activities from the culture and extraction condition of the Spirulina platensis. Korean J Microbiol Biotechnol 34: 143-149
  10. Ciferri O. 1983. Spirulina, the edible microorganism. Microbiol Rev 47: 551-578
  11. Kuhad A, Tirkey N, Pilkhwal S, Chopra K. 2006. Effect of spirulina, a blue green algae, on gentamicin-induced oxidative stress and renal dysfunction in rat. Fund Clin Pharmacol 20: 121-128
  12. 이유경, 이홍금. 2002. 조류(Algae)의 산업적 이용. 생물산업 15: 19-24
  13. Ozdemir G, Karabay NU, Dalay MC, Pazarbasi B. 2004. Antibacterial activity of volatile component and various extracts of Spirulina platensis. Phytother Res 18: 754-757
  14. In MJ, Gwon SY, Chae HJ, Kim DC, Kim DH. 2007. Production of spirulina extract by enzymatic hydrolysis. J Korean Soc Appl Biol Chem 50: 304-307
  15. Heo JC, Park JY, An SM, Lee JM, Yun CY, Shin HM, Kwon TK, Lee SH. 2006. Anti-oxidant and anti-tumor activities of crude extracts by Gastrodia elata Blume. Korean J Food Preserv 13: 83-87
  16. Yoon DH. 1980. Effect of chlorella powder on the growth of lactic acid bacteria. MS Thesis. Korea University, Seoul
  17. Cho EJ, Nam ES, Park SI. 2004. Effect of chlorella extract on acid production and growth of yoghurt starter. Korean J Food Nutr 17: 8-17
  18. Son CW, Shin YM, Sim HJ, Kim MY, Kim MR. 2007. Effect of spirulina on growth of lactic acid bacteria. Korean J Food Cookery Sci 23: 968-976
  19. Kim SK, Baek HC, Byun HG, Kang OJ, Kim JB. 2001. Biochemical composition and antioxidative activity of marine microalgae. J Korean Fish Soc 34: 260-267
  20. Brown MR, Mular M, Miller I, Farmer C, Trenerry C. 1999. The vitamin content of microalgae used in aquaculture. J Appl Phycol 11: 247-255
  21. Lee JN. 1999. Studies on screening of anticancer animmuno-regulating functions from single cell protein, photosynthetic microalgae, Spirulina platensis. MS Thesis. Kangwon University, Kangwondo
  22. Yun YP, Kang WS, Lee MY. 1996. The antithrombotic effects of green tea catechins. J Food Hyg Safety 11: 77-82
  23. Jang IH, In MJ, Chae HJ. 2004. Manufacturing method for traditional doenjang and screening of high fibrin clotting inhibitory samples. J Korean Soc Appl Biol Chem 47: 149-153
  24. Jang MJ, Rhee SJ, Cho SH, Woo MH, Choi JH. 2006. A study on the antioxidative, anti-inflammatory and anti-thrombogenic effects of Zanthoxylum piperitum DC extract. J Korean Soc Food Sci Nutr 35: 21-27

Cited by

  1. Structural characteristics and biological performance of silk fibroin nanofiber containing microalgae spirulina extract vol.101, pp.4, 2014,
  2. Antioxidant activities of chlorella extracts and physicochemi characteristics of spray-dried chlorella powders vol.22, pp.4, 2015,
  3. Comparison of Anti-Inflammatory Activity of Spirulina maxima Extract by Ultrasonication and Water Extraction Process vol.43, pp.12, 2014,
  4. Tri-layered silk fibroin and poly-ɛ-caprolactone small diameter vascular grafts tested in vitro and in vivo vol.23, pp.10, 2015,
  5. Enhancement of Neuroprotective Effects of Spirulina maxima by a Low-temperature Extraction Process with Ultrasonic Pretreatment vol.23, pp.4, 2018,
  6. Composition of Flavonoids and Antioxidative Activity from Juice of Jeju Native Citrus Fruits during Maturation vol.42, pp.3, 2009,