Inhibitory Effects of Gamidaeganghwal-tang(Jiaweidaqianghuo-tang) on Rheumatoid Arthritis Induced by Type II Collagen

가미대강활탕(加味大羌活湯)이 Collagen II로 유발된 관절염에 미치는 영향

  • Kim, Min-Ki (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dae-Jeon University) ;
  • Oh, Min-Suck (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dae-Jeon University)
  • 김민기 (대전대학교 한의과대학 한방재활의학과교실) ;
  • 오민석 (대전대학교 한의과대학 한방재활의학과교실)
  • Received : 2009.03.10
  • Accepted : 2009.04.06
  • Published : 2009.04.30

Abstract

Objectives : This study was carried out to understand the immunity responses and anti-oxidation effect of the Gamidaeganghwal-tang(GDT) on rheumatoid arthritis by using the THP-1 cells and the serum of CIA mice. Methods : For this purpose, GDT was orally administerd to mice with rheumatoid arthritis induced by collagen II. To investigate the immunity responses, value of cytokine and gene expression in the THP-1 cell, levels of cytokines in the serum of CIA(collagen type II induced arthritis) mice, number of immunocyte in PBMC of CIA mice were measured. Then, anti-oxidant activity, scavenging activity on DHHP(2,2-diphenyl-1-picrylhydrazyl) free radical and SOD(Superoxide dismutae)-like activity of GDT was observed. Results : 1. The levels of IL-$1{\beta}$, IL-6, IL-8, MCP-1 at 100, $50{\mu}g/m{\ell}$ of GDT were significantly reduced in the THP-1 cell. 2. The levels of TNF-${\alpha}$, COX-2 mRNA expression at 100, $50{\mu}g/m{\ell}$ of GDT and IL-$1{\beta}$, IL-6 at $100{\mu}g/m{\ell}$ of GDT were significantly reduced in the THP-1 cell line. 3. The levels of IL-6, TNF-${\alpha}$ and IL-$1{\beta}$ were significantly reduced in the serum of CIA mice. 4. The absolute number of CD3+, CD4+ and CD8+ cells were significantly induced, CD3+/CD69+, CD3+/CD49+, CD19+, B220+/CD23+ cells were significantly reduced in PBMC. 5. Scavenging activity on DPPH free radical and SOD-like activity were significantly induced in a concentration dependent manner. Conclusions : Taking all these observations, GDT considered to be effective in treating rheumatoid arthritis. Therefore we have to survey continuously in looking for the effective substance and mechanism in the future.

References

  1. 해리슨 번역 편찬위원회. 해리슨 내과학. 서울:정담출판사. 2006:2148-57.
  2. 한방재활의학과학회. 한방재활의학과학. 서울:군자출판사. 2005:78-99.
  3. 대한정형외과학회. 정형외과학. 서울:최신의학사. 2001:175-6.
  4. 김영태, 이재동, 이윤호. 최근 RA와 관련된 임상 및 실험 논문의 경향. 대한침구학회지. 2002;19(5):190-4.
  5. Schlaak JF, Pfers I, Meyer Zum Buschenfelde KH, Marker-Hermann E. Differant cytokine profiles in the synovial fluid of patient with osteoarthritis, rheumatoid arthritis and seronegative spondyloarthropathies. Clin Exp Rheumarol. 2002;14:63-88.
  6. Merry P, Winyard PG, Morris CJ, Grootveld M, Blake DR. Oxyren free radicals, inflammation and synovitis: the current status. Am Rheum Dis.1989;48:864-70. https://doi.org/10.1136/ard.48.10.864
  7. 羅天益. 衛生寶鑑. 北京:人民衛生出版社. 1983:370-1.
  8. 윤용갑, 김규민, 이성준, 유승훈, 장선일. 금은화 수용성 추출물의 LPS 유도 염증매개물 억제 효과. 대한본초학회지. 2007;22(3):117-25.
  9. 천순주, 조우아, 김영훈, 장민정, 성지연, 강보연, 최은영, 손준호, 백욱진, 이창언, 안봉전, 이진태. 포공영(浦公英)의 항산화 및 화장품약리학적 활성 연구. 대한본초학회지. 2006;21(4):109-13.
  10. 최정식, 송태원, 김동희. 계혈등이 collagen으로 유발된 생쥐의 관절염억제에 관한 연구. 대한본초학회지. 2003;18(3):79-88.
  11. 張仲景. 金要略方論. 臺北:台聯國風出版社. 1973:32-3.
  12. 朱震亨. 丹溪心法附餘. 서울:대성문화사. 1982:206.
  13. 王 燾. 外臺秘要. 北京:黃河出版社. 1993:266.
  14. Schwartz BD. Infectious agents, immunity, and rheumatic disease. Arthritis Rheum. 1990;33:457-65. https://doi.org/10.1002/art.1780330401
  15. Shoenfeld Y, Isenberg DA. Mycobacteria and autoimmunity. Immunol Today. 1988;9:178-81. https://doi.org/10.1016/0167-5699(88)91294-7
  16. 박동준, 김동집. 흰쥐에서 Type II 제2형 Collagen으로 유발된 관절염의 임상양상 및 면역반응. 카톨릭대학의학부 논문집. 1990;43:466-541.
  17. 김호연. 류마티스양 관절염 면역병인의 최근 경향. 서울.대한내과학회지. 1989;36(6):743-5.
  18. Choy EH, Panayi GS. Cytokine pathways and joint inflammation in rheumatoid arthritis. N Engl J Med. 2001;344:907-16. https://doi.org/10.1056/NEJM200103223441207
  19. Paredes S, Girona J, Hunt-Camejo E, Vallve JC, Olive S, Heras M, Bentio P, Masana L. Antioxidant vitamins and lipod peroxidation in patients with Rheumatoid arthritis:association with inflammatory markers. 2002;29(11):2271-7.
  20. Yoichiro Iwakura. Roles of IL-1 in the development of rheumatoid arthritis consideration from mouse models. Cytokine & Growth Factor Review. 2002;13:341-55. https://doi.org/10.1016/S1359-6101(02)00021-7
  21. Abul K. Abdas, Andrew H. Lichtman. 細胞分子免疫學5판. 서울:범문사. 2004:243-53, 261-9, 423-4, 506-12.
  22. Nishimoto N, Kishimoto T. Inhibition of IL-6 for the treatment of inflammatory diseases. Curr. Opin. Pharmacol. 2004;4:386-91. https://doi.org/10.1016/j.coph.2004.03.005
  23. Houssiau FA, Devogelaer JP, VanDamme J, de Deux-chaisnes CN, Van Snick J. Interleukin-6 in synovial fluid and serum of patients with rheumatoid arthritis and other inflammatory arthritides. Arthritis Rheum. 1988;32:784-8.
  24. Baggiolini M, Dewald B, Moser B. Human chemokines. Annu Rev Immunol. 1997;15:675-705. https://doi.org/10.1146/annurev.immunol.15.1.675
  25. Feldmann M, Maini RN. Anti-TNF alpha therapy of rheumatoid arthritis what have we learned. Annu. Rev. Immunol. 2001;19:163-96. https://doi.org/10.1146/annurev.immunol.19.1.163
  26. Pasinatti GM, Alsen PS. Cyclooxygenase-2 expression is increased in frontal cortex of Alzheimer's disease brain. Neuroscience. 1998;87(2):319-24. https://doi.org/10.1016/S0306-4522(98)00218-8
  27. 윤현정, 허숙경, 이영태, 박원환, 박선동. 마우스 대식세포 및 사람 혈관 내피세포에서 오수유(Evodia officinalis DODE) 메탄올 추출물의 항염증 효과. 대한본초학회지. 2008;23(1):29-38.
  28. Charbonmier LM., Duivenvoorde LM., Apparally F. Immature dendritic cell suppress collagen-induced arthritis by in vivo expansion of CD49b+regulatory T cells. J immunol. 2006;177:3806-13. https://doi.org/10.4049/jimmunol.177.6.3806
  29. Varbanova BB. et al. Clinical immunology. New York:Mosby. 2001;4:13,2001;6:18.
  30. Chales A Janeway. 면역생물학(제5판). 서울:라이프사이언스. 2002:698-714.
  31. 이경민, 서정철, 한상원. 황기약침액의 NO, DPPH 消去 및 IL-4 抑制效果. 대한침구학회지. 2003;20(2):184-94.
  32. 이소연, 신유정, 박종혁, 김승모, 박치상. 경옥고의 성분 분석 및 추출물별 항산화 효능 비교. 대한본초학회지. 2008;23(2):123-36.