Anti-Cancer Effects of Salvia Miltiorrhiza, Carydalis Turtschaminovii, Reynoutria Elliptica Herbal Acupuncture on Solid Tumor of Rats induced by Injection of RK3E-ras Cells

RK3E-ras cells로 유발된 흰쥐의 고형종양에 대한 단삼, 현호색, 호장근 약침의 항종양 효과

  • Park, Soo-Gon (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dong-Shin University) ;
  • Shin, Mi-Suk (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dong-Shin University) ;
  • Choi, Jin-Bong (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dong-Shin University) ;
  • Kim, Sun-Jong (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dong-Shin University)
  • 박수곤 (동신대학교 한의과대학 한방재활의학과교실) ;
  • 신미숙 (동신대학교 한의과대학 한방재활의학과교실) ;
  • 최진봉 (동신대학교 한의과대학 한방재활의학과교실) ;
  • 김선종 (동신대학교 한의과대학 한방재활의학과교실)
  • Received : 2008.01.17
  • Accepted : 2009.01.19
  • Published : 2009.01.13
  • 33 0


Abstract

Objectives : The present study was carried out investigate the anti-cancer effect of Salvia miltiorrhiza, Carydalis turtschaminovii and Reynoutria elliptica herbal acupuncture on solid tumor of rats induced by injection of RK3E-ras cells. Methods : RK3E-ras cells were injected on the right lumbar region of rats. After 1 weeks, the experimental rats were divided into four groups : Control group, Salvia miltiorrhiza herbal acupuncture group(SM), Carydalis turtschaminovii herbal acupuncture group(CT), Reynoutria elliptica herbal acupuncture group(RE). And we investigated the weight and size of tumor tissue, gross anatomy, histological and PCNA immunohistochemical study, hepatic and renal metastasis for tumor of each group. Results : 1. In the weight of tumor tissue assessment, SM and CT's weight of tumor tissue was decreased. 2. In the size of tumor tissue assessment, SM was smaller than any other group. 3. In the histological observation, SM's formation of tunica fibrosa that surround the tumor cell was obvious and vasculature that developes circumference of tumor cell was not observed, and density of tumor cell was very low. 4. In the PCNA immunohistochemical study, Control group, SM, RE showed strong immune response in the central site of tumor tissue. 5. In observation of liver and kidney tissue, we were not able to observe tumor cell in the SM. Conclusions : Consequently, SM and CT showed a inhibition of growth and metastasis.

References

  1. 손수곤. 賢兪穴의 鍼刺戟과 黃芪藥鍼이 실험용 생쥐의 면역활성물질인 $IL-1{\alpha}$의 유전자발현에 미치는 影響. 大韓韓醫學會誌. 2000;44:16-25.
  2. 송호섭. 半枝蓮. 大蒜. 白花蛇舌草 複合藥鍼이 腫瘍 및 免疫反應에 미치는 영향. 大韓鍼灸學會誌. 2002;19(4):56-73.
  3. 凌一揆. 中藥本草學. 서울:보건신문사. 1999:485-6, 492-6.
  4. 성락기, 김성훈, 이권익. 丹蔘의 抗癌效果와 活性物質 分離에 關한 硏究. 東醫病理學會誌. 1996;10(2):76-91.
  5. G. Carlsson, B.Gullberg, L. Hafstrom. Estimation of liver tumor volume using different formulas-an experimental study in rats. J. Cancer Res Clin Oncol. 1983;105:20-3. https://doi.org/10.1007/BF00391826
  6. 해리슨 내과학 편찬위원회 편. 해리슨 내과학. 서울:정담. 1997:1963.
  7. K. Wade Foster, Songrong Ren, Iuri D. Louro, Susan M. Lobo-Ruppert, Peggy McKie-Bell, William Grizzle, Martha R. Hayes, Thomas R. Broker, Louise T. Chow and J. Michael Ruppert. Oncogene Expression Cloning by Retroviral Transduction of Adenovirus E1A-immortalized Rat Kidney RK3E Cells: Transformation of a Host with Epithelial Features by c-MYC and the Zinc Finger Protein GKLP. Cell Growth & Differentiation. 1999;1(1):423-34.
  8. J M Ruppert, B Vogelstein, and K W Kinzler. The zinc finger protein CLI transforms primary cells in cooperation with adenovirus E1A. Mol Cell Biol, 1991;11(3):1724-8. https://doi.org/10.1128/MCB.11.3.1724
  9. Christopher J. Torrance, Vijai Agrawal, Bert Vogelstein & Kenneth W. Kinzler. Use of isogenic human cancer cells for high-throughput screening and drug discovery. Nature Biotechnology. 2001;19(1):940-5. https://doi.org/10.1038/nbt1001-940
  10. 대한병리학회. 병리학. 서울 : 고문사. 2003:143.
  11. 서울대학교의과대학편. 종양학 제 2 진정판. 서울:서울대학교출판부. 2001:141-88.
  12. 孫桂芝. 常見腫瘤診治指南. 북경:중국과학기술출판사. 1989:1-4.
  13. 全國韓醫科大學 鍼灸經穴學敎室 編著. 鍼灸學(上.下). 서울:집문당. 1998:485.
  14. 박정현, 김현정, 이지원, 임효권, 이인선. 虎杖根 추출물의 Helicobacter pylori에 대한 생육억제 및 인간 위암세포주에 대한 유전적 손상 유도. 大韓癌豫防學會誌. 2003;8(2):81-90.
  15. 허부. 大戟 分劃 藥鍼이 S-180에 對한 抗腫瘍效果 및 免疫反應에 미치는 影響. 대전대학교 대학원 석사학위논문. 1998.
  16. 한국원자력연구소. 악성종양의 종양 생물학적 연구. 대전:동화사. 1997:178.
  17. 최선미, 최승훈, 안규석. 丹蔘의 抗癌活性과 apoptosis에 미치는 影響. 東醫病理學會誌. 2002;14(2):22-47.
  18. 鬱仁存. 中醫腫瘤學. 북경:과학출판사. 1991:65-74.
  19. F.T. Kolling, G. Hu, C.V. Dang, E.R. Fearon. Neoplastic transformation of RK3E by mutant beta-catenin requires deregulation of Tcf/Lef transcription but not activation of c-myc expression. Mol. Cell. Biol. 1999;19:5696-706. https://doi.org/10.1128/MCB.19.8.5696
  20. 신민교. 임상본초학. 서울:영림사. 1997:519-21, 691-2, 731-2.
  21. 주혜정, 이한구. 한약 증류액의 안정성에 관한 연구. 韓國韓醫學硏究所 論文集. 1995;14(2):106-32.
  22. 배원영. 魚腥草 藥鍼이 B16黑色腫癌모델에 대한 抗腫瘍效果 및 免疫反應에 미치는 影響. 大韓鍼灸學會誌. 2001;18(1):186-201.
  23. 안상우. 약물 주입의 기법적 시원에 대한 소고. 大韓藥鍼學會誌. 1997;1(1):87-101.
  24. 劉建洪, 河冬梅. 穴位藥物注射療法. 江西城:江西科技. 1989:32-3.
  25. 최승훈. 동의종양학. 서울:행림서원. 1995:13-42.
  26. 권기록. 蜂毒藥鍼刺戟이 3-MCA 誘發 上皮種에 對한 抗癌 및 免疫反應에 미치는 影響. 大韓鍼灸學會誌. 1997;14(2):151-72.
  27. 남우열, 전병훈. MMC 등의 抗癌化學療法劑로 誘發된 細胞毒性에 미치는 丹蔘의 影響. 東醫病理學會誌. 1997;11(2):113-7.
  28. 이희재, 김동철, 백승희. 玄胡索이 자궁근종세포의 증식억제와 apoptosis관련 유전자 발현에 미치는 영향. 大韓韓方婦人科學會誌. 2006;19(2):214-25.
  29. 전병욱, 류봉하, 박동원, 류기원. 암종의 병인병리에 관한 문헌적 고찰. 韓方腫瘍學會誌. 1995;1(1):83-101.
  30. Katharine Chapman and David J.. Quantifying the Activation of the Small Molecular Weight G-Protein Ras. HallBiochemistry and Molecular Biology Education. 2005;33(1):22-7. https://doi.org/10.1002/bmb.2005.494033010420
  31. Kronenberg F, Mindes J, Jacobson JS. The future of complementary and alternative medicine for cancer. Cancer invest. 2005;23(5):420-6. https://doi.org/10.1081/CNV-67159
  32. 대한병리학회. 병리학. 서울:고문사. 1995:266.
  33. Paunesku T, Mittal S, Protic M, Oryhon J, Korolev SV, Joachimiak A, Woloschak GE. Proliferating cell nuclear antigen (PCNA): ringmaster of the genome. Radiation Biology. 2001;77(10):1007-21. https://doi.org/10.1080/09553000110069335
  34. 김성훈. 한의학계의 암 연구 동향과 연구 전략에 대한 연구. 大韓韓醫學會誌. 1998;19(1):470-99.
  35. 전국한의과대학 본초학교수 공편. 본초학. 서울:영림사. 1998:413-4, 419-21.