Sensitivity to Ergosterol Biosynthesis Inhibiting-Fungicides of Colletotrichum gloeosporioides Isolated from Persimmon Trees

감나무로부터 분리한 Colletotrichum gloeosporioides의 스테롤 생합성 저해제에 대한 감수성

  • Lim, Tae-Heon (Research Institute of Agri-Bio Science, Samhoub, Co., Ltd.) ;
  • Lee, Dong-Woon (Department of Applied Biology, Kyungpook National University) ;
  • Choi, Yong-Hwa (Department of Plant Resources, Kyungpook National University) ;
  • Lee, Sang-Myeong (Southern Forest Research Center, Korea Forest Research Institute) ;
  • Han, Sang-Sub (Department of Forest Resources, Chonbuk National University) ;
  • Cha, Byeong-Jin (Department of Plant Medicine, Chungbuk National University)
  • 임태헌 ((주) 삼호유비 농생명과학연구소) ;
  • 이동운 (경북대학교 생물응용학과) ;
  • 최용화 (경북대학교 식물자원학과) ;
  • 이상명 (국립산림과학원 남부산림연구소) ;
  • 한상섭 (전북대학교 산림자원학과) ;
  • 차병진 (충북대학교 식물의학과)
  • Published : 2009.09.30

Abstract

In 2008, 110 isolates of Colletotrichum gloeosporioides were obtained from infected twigs of persimmon collected at Sangju and five fungicides (prochloraz manganese complex, tebuconazole, mancozeb+myclobutanil, fluquinconazole+prochloraz, and tebuconazole+tolyfluanid) were evaluated to determine their growth on fungicide-medium. Among them, the mycelial growth of 97.3 and 98.2% of isolates was inhibited over 91% in response to prochloraz ($250\;{\mu}g/m{\ell}$) and tebuconazole ($125\;{\mu}g/m{\ell}$), respectively, compared to untreated control. In response to mancozeb+myclobutanil, fluquinconazole+prochloraz, and tebuconazole+tolyfluanid, isolates of 96.4, 99.1 and 96.4% of them were inhibited by fungicides, respectively. Isolates showed the highest sensitivity to fluquinconazole+prochloraz among 5 fungicides. The correlation between tebuconazole and tebuconazole+tolyfluanid was higher (r=0.85).

References

  1. Adaskaveg, J. E. and H. Forsyer (2000) Occurrence and management of anthracnose epidemics caused by Colletotrichum species on tree fruits crops in Califomia. pp.300-316 in:Colletotrichum-host specificity, pathology and host-pathogen interaction. D. Prusky, S. Freeman and M. B. Dickman, eds. APS Press, Minnesota. USA
  2. Brent, K. J. and D.W. Hollomon (1998) Fungicide resistance:The assessment of risk. FRAC. 48pp
  3. Delp, C. J. (1988) Fungicide resistance in North America. The American Phytopathological Society, St. Paul, Mn. 133pp
  4. Fletcher, J. S., and M. S. Wolfe (1981) Insensitivity of Erysiphe graminis f.sp. hordei to triadimefon, triadimenol and other fungicides. Brighton Crop Protection Conference; pests and diseases-1981, 2:633-640
  5. Fry, W. E. (1982) Principles of plant disease management. Academic Press, Orlando. 378pp
  6. Hargreaves, J. A., J. P. R. Keon and R. Croxen (1996) Molecular genetics of ergosterol biosynthesis in Ustilago maydis. pp.117-123. in Modem fungicides and antifungal compounds. H. Lyr, P. E. Russell and H. D. Sisler, eds. Intercept Ltd., Andover, UK
  7. Lim, T. H., T. H. Chang and B. J. Cha (1998) Incidence of benzimidazole- and dicarboximide-resistant isolates of Monilinia fructicola from overwintering mummies and peduncles on peach trees. Korean. J. Plant Pathol. 14:367-370
  8. Staub. T. (1991) Fungicide resistance: Practical experience with antiresistance strategies and the role of integrated use. Ann. Rev. Phytopathol. 29:421-442 https://doi.org/10.1146/annurev.py.29.090191.002225
  9. Tomlin, C. D. S. (2006) Prochloraz pp. 859. in: The pesticide manual fourteen edition. Published by BCPC. UK
  10. Yasunori, T. and H. Ishii (1998). Reduced sensitivity to fenarimol in Japanese filed strains of Venturia nashicola. Pestic. Sci. 54:150-156 https://doi.org/10.1002/(SICI)1096-9063(1998100)54:2<150::AID-PS797>3.0.CO;2-A
  11. 김태춘, 이규철, 이용문 (2002) 감의 생리생태와 재배신기술. 중앙생활사. 332pp
  12. 박성우, 김준태, 김재정, 김흥태 (2002) 고추에서 분리한 탄저병균의 스테롤 생합성 저해 살균제에 대한 감수성 반응과 포장 적응력. 식물병연구 8:239-244 https://doi.org/10.5423/RPD.2002.8.4.239
  13. 상주시 (2007) 상주시 통계연보. 상주시. 459pp
  14. 이동운, 이상명, 최병렬, 박정규, 추호렬 (2003) 단감원의 최근 농약사용실태. 한국응용곤충학회지 42:85-89
  15. 임태헌, 최용화 (2006) 상주지역 감나무로부터 분리한 Colletotrichum gloeosporioides 균주들의 방제 약제에 대한 반응. 식물병연구 12:99-102
  16. 임태헌, 최용화, 송인규, 김국래, 이동운, 이상명 (2008) 2007년과 2008년 경북 상주지역 감 과원의 과원 관리실태 조사. 한국농약과학회지 12:414-420
  17. 한국균학회 (1999) 균학개론. 321pp
  18. 한국작물보호협회 (2008) 농약사용지침서. 1080pp