Optimization of Supercritical Fluid Extraction of Tocotrienol from Grape Seed

초임계유체 추출을 이용한 포도씨 tocotrienol 추출조건 최적화

  • Published : 2009.02.28


In this study, supercritical carbon dioxide extraction (SFE) was utilized for the extraction of tocotrienol from grape seeds. The optimal conditions for vitamin E and tocotrienol extraction were determined via response surface methodology (RSM). Central composite design was utilized to assess the effects of oven temperature (30-$50^{\circ}C$, X1), operating pressure (17-25 MPa, X2), and extraction time (1-5 hr, X3) of supercritical fluid extraction. Vitamin E and tocotrienol contents were 8.65 mg/100 g and 7.88 mg/100 g at $40^{\circ}C$, 20MPa and 5 hr, respectively. The predicted extraction condition was validated via actual experimentation. The predicted extraction conditions were $40^{\circ}C$, 3.8 hr, and 20.7MPa. The vitamin E and tocotrienol contents under these conditions were 8.20 mg/100 g and 7.42 mg/100 g, respectively. The vitamin E and tocotrienol contents of solvent extraction with hexane were 8.18 mg/100 g and 7.24 mg/100 g, respectively.


grape seed;supercritical fluid extraction;response surface methodology;vitamin E;tocotrienol


  1. Choi SW, Chung US, Lee KT. Preparation of high quality grape seed oil by solvent extraction and chemical refining process. Korean J. Food Preserv. 12: 600-607 (2005)
  2. Shin MK, Hong JH, Hur SS, Choi YH. Extraction of $\beta$-carotene in co-solvent induced SFE process. Food Eng. Prog. 6: 301-307 (2002)
  3. Kim SJ, Kim YJ, Chang KS. Optimization of sesame oil extraction from sesame cake using supercritical fluid$CO_{2}$. Korean J. Food Sci. Technol. 37: 431-437 (2005)
  4. Lee EJ, Kwon JH. Characteristics of microwave-assisted extraction for grape seed components with different solvent. Korean J. Food Preserv. 13: 216-222 (2006)
  5. Pekic B, Kovac V, Alonso E, Revilla E. Study of the extraction of proanthocyanidins from grape seeds. Food Chem. 61: 201-206 (1998) https://doi.org/10.1016/S0308-8146(97)00128-3
  6. Jang JK, Han JY. The antioxidant ability of grape seed extracts. Korean J. Food Sci. Technol. 34: 524-528 (2002)
  7. Kim YK, Lee HY, Oh DH. Changes in antioxidative activity and total polyphenols of crude and defatted grape seed extract by extraction condition and storage. Korean J. Food Preserv. 11:455-460 (2004)
  8. Jang JK, Han JY. The antioxidant ability of grape seed extracts. Korean J. Food Sci. Technol. 34: 524-528 (2002)
  9. Lee DJ, Lee JY. Tocopherols and tocotrienol in cereal grains. Korean J. Crop Sci. 48: 1-12 (2003)
  10. Kim KM, Choi SG, Lee JS, Jeong HS. Effects of extraction conditions on the tocotrienol content of grape seed extract. J. Agr.Life Sci. 41: 25-31 (2007)
  11. Cochran WG, Cox GM. Experimental designs. 2nd edition, John Whily & Sons, Inc. New York. London. (1950)
  12. Park SJ, Lee HY, Oh DH. Free radical scavenging effect of seed and skin extracts from campbell early grape (Vitis labruscana B.). J. Korean Soc. Food Sci. Nutr. 32: 115-118 (2003) https://doi.org/10.3746/jkfn.2003.32.1.115
  13. Park KY, Kang CS, Lee YS, Lee YH, Lee YS. Tocotrienol and tocopherol content in various plant seeds. Korean J. Crop Sci. 49:207-210 (2004)
  14. Juncheng L, Wei W, Ganzuo L. A new strategy for supercritical fluid extraction of copper ions. Talanta. 53: 1149-1154 (2001) https://doi.org/10.1016/S0039-9140(00)00607-X
  15. Ju YW, Son MH, Lee JS, Byun SY. Supercritical CO2 extraction of sesame oil with high content of tocopherol. Korean J. Biotechnol. Bioeng. 20: 210-214 (2005)
  16. Woo MJ, Seo JW, Byun SY. Extraction of resveratrol containing grape seed oil with supercritical carbon dioxied. Korean J. Biotechnol. Bioeng. 20: 383-386 (2005)
  17. Lee H, Chung BH, Park YH. A fundamental study of supercritical fluid extraction process for concentrating tocopherols from soybean sludge. Korean chem. Eng. Res. 29: 206-210 (1991)
  18. Lee WY, Chang KS, Choi YH. Extraction of phenolic compounds from grape seed using supercritical CO2 and ethanol as a Co-solvent. Korean J. Postharvest Sci. Technol. 7: 177-183 (2000)