보성산 유기농 녹차의 품질에 따른 카테킨 함량과 항산화능 비교 분석

Comparative Analysis of Catechins and Antioxidant Capacity in Various Grades of Organic Green Teas Grown in Boseong, Korea

  • 박경련 (경희대학교 식품공학과 및 생명자원과학연구원) ;
  • 이상길 (경희대학교 식품공학과 및 생명자원과학연구원) ;
  • 남태규 (경희대학교 식품공학과 및 생명자원과학연구원) ;
  • 김영준 (고려대학교 식품생명공학과) ;
  • 김영록 (경희대학교 식품공학과 및 생명자원과학연구원) ;
  • 김대옥 (경희대학교 식품공학과 및 생명자원과학연구원)
  • Park, Kyung-Ryun (Department of Food Science and Technology and Institute of Life Science and Resources, Kyung Hee University) ;
  • Lee, Sang-Gil (Department of Food Science and Technology and Institute of Life Science and Resources, Kyung Hee University) ;
  • Nam, Tae-Gyu (Department of Food Science and Technology and Institute of Life Science and Resources, Kyung Hee University) ;
  • Kim, Young-Jun (Department of Food and Biotechnology, Korea University) ;
  • Kim, Young-Rok (Department of Food Science and Technology and Institute of Life Science and Resources, Kyung Hee University) ;
  • Kim, Dae-Ok (Department of Food Science and Technology and Institute of Life Science and Resources, Kyung Hee University)
  • 발행 : 2009.02.28

초록

유기농 녹차의 등급별 총페놀 함량과 항산화능은 각 추출 용매에 따른 수율을 확인하였을 때 물 추출에 비하여 수용성 유기 용매의 추출 수율이 유의적으로 높았다. 유기용매를 이용한 추출 수율은 물을 이용할 때 보다 총페놀 함량은 1.5에서 3.2배, 항산화능은 1.8에서 3.8배 정도 높게 나타났다. HPLC 분석을 통하여 총페놀 함량과 항산화능의 상관관계를 비교 시 총페놀 함량이 증가함에 따라 항산화능이 1차 선형관계로 증가하는 경향을 보였다. HPLC분석을 통하여 유기농 녹차에 함유되어 있는 다양한 카테킨의 함량이 채엽시기에 따라 변화가 나타났다. 유기농 녹차의 카테킨 성분 중 가장 많이 존재하는 EGCG가 건조중량당 5.8-7.7%의 함량을 보였고, caffeine은 1.7-2.9%의 함량을 보였다. 유기농 녹차의 카테킨 함량은 일반적으로 채엽 시기가 늦어짐에 따라서 점차 감소하였다. 유기농 녹차의 등급에 따른 항산화능은 최상급인 우전에서 가장 높았으며 최하등급인 엽차에서 가장 낮게 나타났다. HPLC 분석에 의한 유기농 녹차의 등급에 따른 총 카테킨 함량(mg/g)은 우전(155.4), 세작(147.7), 중작(143.2), 엽차(135.1), 대작(130.5)의 순서로 감소하였다. 이를 통해 녹차의 채엽 시기가 유기농 녹차의 카테킨 성분 및 항산화능에도 영향을 미치는 것을 확인하였다. 전반적으로 상급의 유기농 녹차 일수록 총페놀 및 카테킨 함량이 많으며 또한 더 높은 항산화능을 가지는 것으로 나타났다.

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