Antioxidant Properties and Protective Effects of Inula britannica var. chinensis Regel on Oxidative Stress-induced Neuronal Cell Damage

금불초 추출물의 항산화 효과 및 산화 스트레스에 대한 신경세포 보호작용

  • Lee, Na-Hyun (Division of Food Science, College of Natural Science, Seoul Women's University) ;
  • Hong, Jung-Il (Division of Food Science, College of Natural Science, Seoul Women's University) ;
  • Kim, Jin-Yung (Division of Environment and Life Science, College of Natural Science, Seoul Women's University) ;
  • Chiang, Mae-Hee (Division of Environment and Life Science, College of Natural Science, Seoul Women's University)
  • 이나현 (서울여자대학교 식품과학부) ;
  • 홍정일 (서울여자대학교 식품과학부) ;
  • 김진영 (서울여자대학교 환경생명과학부) ;
  • 장매희 (서울여자대학교 환경생명과학부)
  • Published : 2009.02.28


The antioxidant properties and protective effects of Inula britannica on ${H_2}{O_2}$-induced SH-SY5Y neuroblastoma cell damage were investigated. A series of solvent fractions, including hexane(Fr.H), petroleum ether, chloroform, ethyl acetate(Fr.EA), and water fraction(Fr.W), were prepared from the 70% methanol extracts of Inula britannica. Fr.W had the highest total contents of phenolics and flavonoids, followed by Fr.EA. The antioxidant properties of the fractions were also evaluated by analyzing their scavenging activities on 1,1-diphenyl-2-picrylhydrazyl(DPPH) radicals, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals, and nitric oxide. Fr.W showed the strongest activities in all assays. The concentrations of Fr.W that resulted in 50% reductions of the DPPH and ABTS radicals were 20.7 ${\mu}g$/mL and 39.4 ${\mu}g$/mL, respectively. Fr.W showed the weakest cytotoxic activities on the SH-SY5Y cells, whereas it effectively protected ${H_2}{O_2}$-induced cell death, increasing cell survival by 35.0-77.0% at a concentration range of 62.5-250 ${\mu}g$/mL. In this range, Fr.W also significantly decreased intracellular ROS levels by 34-39%. Overall, the antioxidant properties of Inula britannica can contribute to rescuring neuronal cells from oxidative stress-induced cell injury.


Inula britannica;polyphenol;reactive oxygen species;neuroprotective effect;SH-SY5Y


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