Antioxidant Properties and Protective Effects of Inula britannica var. chinensis Regel on Oxidative Stress-induced Neuronal Cell Damage

금불초 추출물의 항산화 효과 및 산화 스트레스에 대한 신경세포 보호작용

  • Lee, Na-Hyun (Division of Food Science, College of Natural Science, Seoul Women's University) ;
  • Hong, Jung-Il (Division of Food Science, College of Natural Science, Seoul Women's University) ;
  • Kim, Jin-Yung (Division of Environment and Life Science, College of Natural Science, Seoul Women's University) ;
  • Chiang, Mae-Hee (Division of Environment and Life Science, College of Natural Science, Seoul Women's University)
  • 이나현 (서울여자대학교 식품과학부) ;
  • 홍정일 (서울여자대학교 식품과학부) ;
  • 김진영 (서울여자대학교 환경생명과학부) ;
  • 장매희 (서울여자대학교 환경생명과학부)
  • Published : 2009.02.28

Abstract

The antioxidant properties and protective effects of Inula britannica on ${H_2}{O_2}$-induced SH-SY5Y neuroblastoma cell damage were investigated. A series of solvent fractions, including hexane(Fr.H), petroleum ether, chloroform, ethyl acetate(Fr.EA), and water fraction(Fr.W), were prepared from the 70% methanol extracts of Inula britannica. Fr.W had the highest total contents of phenolics and flavonoids, followed by Fr.EA. The antioxidant properties of the fractions were also evaluated by analyzing their scavenging activities on 1,1-diphenyl-2-picrylhydrazyl(DPPH) radicals, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals, and nitric oxide. Fr.W showed the strongest activities in all assays. The concentrations of Fr.W that resulted in 50% reductions of the DPPH and ABTS radicals were 20.7 ${\mu}g$/mL and 39.4 ${\mu}g$/mL, respectively. Fr.W showed the weakest cytotoxic activities on the SH-SY5Y cells, whereas it effectively protected ${H_2}{O_2}$-induced cell death, increasing cell survival by 35.0-77.0% at a concentration range of 62.5-250 ${\mu}g$/mL. In this range, Fr.W also significantly decreased intracellular ROS levels by 34-39%. Overall, the antioxidant properties of Inula britannica can contribute to rescuring neuronal cells from oxidative stress-induced cell injury.

Keywords

Inula britannica;polyphenol;reactive oxygen species;neuroprotective effect;SH-SY5Y

References

  1. Song KY. Yasenghwa shypge chatgi (Convenient searching for wild plants). Jinsun corp., Seoul, Korea. pp. 355 (2003)
  2. Encyber. Inula britannica var. chinensis. Available from: http://www.encyber.com. Accessed Aug. 11, 2008
  3. Iijima K, Kiyohara H, Tanaka M, Matsumoto T, Cyong JC, Yamada H. Preventive effect of taraxasteryl acetate from Inula britannica subsp. Japonica on experimental hepatitis in vivo. Planta Med. 61: 50-53 (1995) https://doi.org/10.1055/s-2006-957998
  4. Han WJ, Lee BG, Kim YK, Yoon JW, Jin HK, Hong S, Lee HY, Lee KR, Lee HW. Ergolide, sesquiterpene lactone from Inula britannica, inhibits inducible nitric oxide synthase and cyclo-oxygenase-2 expression in RAW 264.7 macrophages through the inactivation of NF-kappa B. Brit. J. Pharmacol. 133: 503-512 (2001) https://doi.org/10.1038/sj.bjp.0704099
  5. McKee T, McKee JR. Biochemistry. 3th ed. McGraw-Hill, New York, NY, USA. pp. 319-325 (2002)
  6. Choi WH, Oh YS, Ahn JY, Kim SR, Ha TY. Antioxidative and protective effects of Ulmus davidiana var. japonica extracts on glutamate-induced cytotoxicity in PC 12 cells. Korean J. Food Sci. Technol. 37: 479-483 (2005)
  7. Yoon MY, Kim JY, Hwang JH, Cha MR, Lee MR, Jo KJ, Park HR. Protective effect of methanolic extracts from Dendrobium nobile Lindl. on H2O2-induced neurotoxicity in PC12 cells. J. Korean Soc. Appl. Biol. Chem. 50: 63-67 (2007)
  8. Kim NY, Lee JH, Heo MY. Protective effect of green teaextracts on oxidative stress. Korean J. Med. Crop Sci. 14: 322-328 (2006)
  9. Xie JT, Shao ZH, Vanden Hoek TL, Chang WT, Li J, Mehendale S, Wang CZ, Hsu CW, Becker LB, Yin JJ, Yuan CS. Antioxidant effects of ginsenoside Re in cardiomyocytes. Eur. J. Pharmacol. 532: 201-207 (2006) https://doi.org/10.1016/j.ejphar.2006.01.001
  10. Kim NM, Sung HS, Kim WJ. Effect of solvents and some extraction conditions on antioxidant activity in cinnamon extracts. Korean J. Food Sci. Technol. 25: 204-209 (1993)
  11. Huang SH, Lin CM, Chiang BH. Protective effects of Angelica sinensis extract on amyloid beta-peptide-induced neurotoxicity. Phytomedicine 15: 710-721 (2008) https://doi.org/10.1016/j.phymed.2008.02.022
  12. Kim SR, Park MJ, Lee MK, Sung SH, Park EJ, Kim J, Kim SY, Oh TH, Markelonis GJ, Kim YC. Flavonoids of Inula britannica protect cultured cortical cells from necrotic cell death induced by glutamate. Free Radical Bio. Med. 32 : 596-604 (2002) https://doi.org/10.1016/S0891-5849(02)00751-7
  13. Park EJ, Kim J. Cytotoxic sesquiterpene lactones from Inula britannica. Planta Med. 64: 752-754 (1998) https://doi.org/10.1055/s-2006-957573
  14. Blios MS. Antioxidant determination by the use of a stable freeradical. Nature 26: 1199-1203 (1958)
  15. Park EJ, Kim Y, Kim J. Acylated flavonol glycosides from the flower of Inula britannica. J. Nat. Prod. 63: 34-36 (2000) https://doi.org/10.1021/np990271r
  16. Hong JI, Kweon MH, Ra KS, Sung HC, Yang HC. Free radical scavenging activities and inhibitory effects on xanthine oxidase by ethanol extract from Capsella bursa-pastoris. Agr. Chem. Biotechnol. 38: 590-595 (1995)
  17. Dewanto V, Wu X, Liu RH. Processed sweet corn has higherantioxidant activity. J. Agr. Food Chem. 50: 4959-4964 (2002) https://doi.org/10.1021/jf0255937
  18. Kobayashi T, Song QH, Hong T, Kitamura H, Cyong JC. Preventive effects of the flowers of Inula britannica on autoimmune diabetes in C57B/KsJ mice induced by multiple low doses of streptozotocin. Phytother. Res. 16: 377-382 (2002) https://doi.org/10.1002/ptr.868
  19. Lee YM. Hankuk eui yasenghwa (Korean wild plants). Dareunsesang, Seoul, Korea. pp. 245-248 (2003)
  20. Lee SO, Lee HJ, Yu MH, Im HG, Lee IS. Total polyphenol contents and antioxidant activities of methanol extracts from vegetables produced in Ullung island. Korean J. Food Sci. Technol. 37:233-240 (2005)
  21. Kim HY, Woo KS, Hwang IG, Lee YR, Jeong HS. Effects of heat treatments on the antioxidant activities of fruits and vegetables. Korean J. Food Sci. Technol. 40: 166-170 (2008)
  22. Zha J, Fu Y, Wu Y, Guo C, Zhang D, Wang Y. Study of chemical constituents of the essential oil from Inula britannica L. by GC-MS. Zhong Yao Cai. 28: 466-468 (2005)
  23. Jia Z, Tang M, Wu J. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 64: 555-559 (1999) https://doi.org/10.1016/S0308-8146(98)00102-2
  24. Gray JI, Dugan LR. Inhibition of N-nitrosamine formation in model food systems. J. Food Sci. 40: 981-984 (1975) https://doi.org/10.1111/j.1365-2621.1975.tb02248.x
  25. Han M, Wen JK, Zheng B, Zhang DQ. Acetylbritannilatone suppresses NO and PGE$PGE_2$2 synthesis in RAW 264.7 macrophages through the inhibition of iNOS and COX-2 gene expression. Life Sci. 75 : 675-684 (2004)
  26. Rafi MM, Bai NS, Ho CT, Rosen RT, White E, Perez D, Dipaola RS. A sesquiterpenelactone from Inula britannica induces antitumor effects dependent on Bcl-2 phosphorylation. Anticancer Res. 25: 313-318 (2005)