Detection of Peanuts in Commercially Processed Foods by an Enzyme-Linked Fluorescent Immunoassay

Enzyme-linked fluorescent immunoassay에 의한 가공식품 중 땅콩의 검출

  • Published : 2009.02.28

Abstract

In this study we analysed for peanuts in processed foods using an enzyme-linked fluorescent immunoassay (ELFA), and compared the results with labeled ingredients. Crude peanut protein (CPP) was immunized into rabbits to produce specific antibodies(Ab). A sandwich ELFA was established using anti-CPP Ab and Ab-horseradish peroxidase (HRP) conjugate. The cross-reactivities of the Ab toward CPP, peanuts, almonds, soybeans, and walnuts were 100, 9.8, $1.1{\times}10^{-2},\;4.4{\times}10^{-3}$, and 0%, respectively. The samples included 19 items consisting of biscuits, snacks, chocolates, and so on. The results from the sandwich ELFA showed that peanuts were contained in 7 of the processed food items, among which, 5 items were labeled as having peanuts present but 2 items were not. One of the 2 items that was peanut-detected but unlabeled was a biscuit labeled to contain almonds and assayed to contain $2.1{\times}10^{-3}%$ peanuts, which might have been due to the weak cross-reactivity of the Ab toward almonds. The other item was a snack labeled to contain soybeans and assayed to contain 0.098% peanuts, which might have been due to peanut cross-contamination during processing, since the crossreactivity of the Ab toward soybeans was very weak. These results suggest that ELFA is a good tool to detect peanuts in processed foods, and allergens in certain processed foods should be labeled correctly.

Keywords

peanuts;detection;specific antibody;enzyme-linked fluorescent immunoassay (ELFA)

References

  1. Werfel T. Food allergy. J. Dtsch. Dermatol. Ges. 6: 573-583 (2008) https://doi.org/10.1111/j.1610-0387.2008.06778.x
  2. Host A, Haiken S. A prospective study of cow milk allergy in Danish infants during the first three years of life. Clinical course in relation to clinical and immunological type of hypersensitivity reaction. Allergy 45: 587-596 (1990) https://doi.org/10.1111/j.1398-9995.1990.tb00944.x
  3. Sampson HA, McCaskill C. Food hypersensitivity in atopic dermatitis evaluation of 113 patients. J. Pediatr. 107: 669-675 (1985) https://doi.org/10.1016/S0022-3476(85)80390-5
  4. Sampson HA, Mendelson L, Rosen JP. Fatal and near-fatal anaphylactic reactions to food in children and adolescents. Engl. J.Med. 327: 380-384 (1992) https://doi.org/10.1056/NEJM199208063270603
  5. Korea Food and Drug Administration. Labeling Standards for Foods etc. KFDA Notification No. 2008-31 (Amendment on Jun.17, 2008) Available from:http://www.kfda.go.kr/open_content/data/food_view.php. Accessed Dec. 1, 2008
  6. Pomes A, Helm RM, Bannon GA, Burks AW, Tsay A, Champman MD. Monitoring peanut allergen in food products by measuring Ara h1. J. Allergy Clin. Immunol. 12: 640-645 (2003)
  7. Zaitsu K, Ohkura Y. New fluorogenic substrates for horseradish peroxidase: Rapid and sensitive assays for hydrogen peroxide and the peroxidase. Anal. Biochem. 109: 109-113 (1980) https://doi.org/10.1016/0003-2697(80)90017-2
  8. Koppelman SJ, Wensing M, Ertmann M, Knulst AC, Knol EF. Relevance of Ara h1, Ara h2, and Ara h3 in peanut-allergic patients, as determined by immunoglobulin E western blotting, basophilhistamine release and intracutaneous testing: Ara h2 is the most important peanut allergen. Clin. Exp. Allergy 34: 583-590 (2004) https://doi.org/10.1111/j.1365-2222.2004.1923.x
  9. de Leon MP, Glaspole IN, Drew AC, Rolland JM, O'Hehir RE, Suphioglu C. Immunological analysis of allergenic cross-reactivity between peanut and tree nuts. Clin. Exp. Allergy 33: 1273-1280 (2003) https://doi.org/10.1046/j.1365-2222.2003.01761.x
  10. Burks AW. Peanut allergy. Lancet 371: 1538-1546 (2008) https://doi.org/10.1016/S0140-6736(08)60659-5
  11. Tresca JP, Ricoux R, Pontet M, Engler R. Comparative activity of peroxidase-antibody conjugates with periodate and glutaraldehyde coupling according to an enzyme immunoassay. Ann. Biol. Clin. 53:227-231 (1995)
  12. Stephan O, Vieths S. Development of a real-time PCR and a sandwich ELISA for detection of potentially allergic trace amounts of peanut(Arachis hypogaea) in processed foods. J. Agr. Food Chem.52: 3754-3760 (2004) https://doi.org/10.1021/jf035178u
  13. Yolken RH, Stopa PJ. Enzyme-Linked Fluorescence Assay: Ultrasensitive solid-phase assay for detection of human rotavirus. J. Clin. Microbiol. 10: 317-321 (1979)
  14. Kiening M, Niessner R, Drs E, Baumgartner S, Krska R, Bremer M, Tomkies V, Reece P, Danks C, Immer U, Weller MG. Sandwich immunoassays for the determination of peanut and hazelnuttraces in foods. J. Agr. Food Chem. 53: 3321-3327 (2005) https://doi.org/10.1021/jf048394r
  15. Shon DH. Food and allergy. Food Sci. Ind. 33: 1-8 (2000) https://doi.org/10.1111/j.1365-2621.1968.tb00872.x
  16. Poms RE, Klein CL, Anklam E. Polymerase chain reaction techniques for food allergen detection. Food Addit. Contam. 21: 1-31 (2004) https://doi.org/10.1080/02652030310001620423
  17. Laemmli UK. Cleavage of structural proteins during the assemblyof the head of bacteriophage T4. Nature 227: 680-685 (1970) https://doi.org/10.1038/227680a0
  18. Kim HJ, Shon DH, An enzyme-linked immunosorbent assay for detection of cooked goat meat. Korean J. Food Sci. Technol. 32:538-543 (2000)