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Monitoring the Change of Protein Expression in Human Colon Cancer Cell SNU-81 treated with the Water-Extract of Coptis japonica

황련 열수추출물을 처치한 인간 대장암 세포 SNU-81에서의 단백질 발현 변화

  • Yoo, Tae-Mo (College of Oriental Medicine, Daejeon University) ;
  • Kim, Byung-Soo (College of Oriental Medicine, Daejeon University) ;
  • Yoo, Byong-Chul (Research Institute, National Cancer Center) ;
  • Yoo, Hwa-Seung (East-West Cancer Center, Dunsan Oriental Medical Hospital, Daejeon University)
  • 유태모 (대전대학교 한의과대학) ;
  • 김병수 (대전대학교 한의과대학) ;
  • 유병철 (국립암센터 연구소) ;
  • 유화승 (대전대학교 둔산한방병원 동서암센터)
  • Published : 2009.03.30

Abstract

Background : Anticancer effects of herbal medicine have been reported in various types of cancer, but the systematic approaches to explain molecular mechanism(s) are not established yet. Objective : To find the anticancer-effect and mechanism(s) of Water Extract of Coptis japonica (WECJ) colon cancer cell (SNU-81). Methods : We first selected 11 herbals, and anti-cancer effects of water-extracts from those herbals have been tested in human colon cancer cell line, SNU-81. Among the tested herbals, the WECJ significantly reduced proliferation of SNU-81. To establish a basis of understanding for anti-cancer mechanism, whole proteins have been obtained from SNU-81 harvested at 48 and 96 hrs after the treatment of WECJ, protein expression has been profiled by 2DE-based proteomic approach. Results : Various changes of the protein expression have been monitored, and most frequent dysregulation was found in the molecular chaperons including heat shock protein 90-alpha (Hsp90-alpha), 14-3-3 protein epsilon, T-complex protein 1 subunit alpha, protein disulfide-isomerase A3, and calreticulin. Interestingly, proliferation-associated protein 2G4 has been up-regulated, and it suggests the possible effect of Coptis japonica on ErbB3-regulated signal transduction pathway and growth control of human colon cancer cells. Conclusion : Based upon the present findings, the further study will focus on monitoring various cancer survival factors after artificial regulation of the proteins identified, and it would be the basis for the understanding of the Coptis japonica anti-cancer effect(s) at the molecular level.

References

  1. Koo, H.N., H.J. Jeong, I.Y. Choi, H.J. An, P.D. Moon, S.J. Kim, S.Y. Jee, J.Y. Um, S.H. Hong, S.S. Shin, D.C. Yang, Y.S. Seo and H.M. Kim. Mountain grown ginseng induces apoptosis in HL-60 cells and its mechanism have little relation with TNF-alpha production. Am. J. Chin. Med. 2007;35:169-182 https://doi.org/10.1142/S0192415X07004710
  2. M. Ou. Chinese-English manual of commonused traditional medicine, Guangdong Science and Technology Publishing House, Guangzhou, 548-549, 1992
  3. Gao, M., C. Deng, S. Lin, F. Hu, J. Tang, N. Yao and X. Zhang. Recent developments and contributions from Chinese scientists in multidimensional separations for proteomics and traditional Chinese medicines. J. Sep. Sci. 2007;30:785-791 https://doi.org/10.1002/jssc.200600372
  4. Wen, J.K. and M. Han. Application of genomics and proteomics in study of traditional Chinese medicine. Zhong Xi Yi Jie He Xue Bao 2004;2:323-325 https://doi.org/10.3736/jcim20040502
  5. Kang YJ. Herbogenomics: from traditional Chinese medicine to novel therapeutics. Exp Biol Med (Maywood). 2008;233(9):1059-1065 https://doi.org/10.3181/0802-MR-47
  6. Liu CB, Chen AH, Ding YJ, Jin HY, Seow-Choen F. The role of traditional Chinese medicine in colorectal cancer treatment. Tech Coloproctol. 2008 Mar;12(1):1-6; discussion 6 https://doi.org/10.1007/s10151-008-0392-z
  7. Fukuda K, Hibiya Y, Mutoh M, Koshiji M, Akao S, Fujiwara H. Inhibition by berberine of cyclooxygenase-2 transcriptional activity in human colon cancer cells. J Ethnopharmacol. 1999 Aug;66(2):227-233 https://doi.org/10.1016/S0378-8741(98)00162-7
  8. Park HJ, Kim YJ, Leem K, Park SJ, Seo JC, Kim HK, Chung JH. Coptis japonica root extract induces apoptosis through caspase3 activation in SNU-668 human gastric cancer cells. Phytother Res. 2005 Mar;19(3):189-192 https://doi.org/10.1002/ptr.1539
  9. De Maio A. 'Heat shock proteins: facts, thoughts, and dreams'. Shock (Augusta, Ga.) 11 (1): 1-12. 1999 https://doi.org/10.1097/00024382-199901000-00001
  10. Li Z, Srivastava P. Heat-shock proteins. Current protocols in immunology. edited by John E. Coligan et al. Appendix 1: Appendix 1T, 2004
  11. Santoro MG. h Heat shock factors and the control of the stress response. Biochemical pharmacology 2000;59(1):55-63 https://doi.org/10.1016/S0006-2952(99)00299-3
  12. Borges JC, Ramos CH. Protein folding assisted by chaperones. Protein and peptide letters. 2005;12(3):257-261 https://doi.org/10.2174/0929866053587165
  13. Ito A, Saito H, Mitobe K, Minamiya Y, Takahashi N, Maruyama K, Motoyama S, Katayose Y, Ogawa JI. Inhibition of heat shock protein 90 sensitizes melanoma cells to thermosensitive ferromagnetic particle-mediated hyperthermia with low Curie temperature. Cancer Sci. 2009 Jan 14.[Epub ahead of print] https://doi.org/10.1111/j.1349-7006.2008.01072.x
  14. Http://www.uniprot.org/uniprot/P17987
  15. Http://rat.embl.de/rat/P115/P11598.htm
  16. Yaffe MB, Farr GW, Miklos D, Horwich AL, Sternlicht ML, Sternlicht H. TCP1 complex is a molecular chaperone in tubulin biogenesis. Nature. 1992 Jul 16;358(6383):245-248 https://doi.org/10.1038/358245a0
  17. Http://en.wikipedia.org/wiki/Calreticulin
  18. Kepp O, Tesniere A, Schlemmer F, Michaud M, Senovilla L, Zitvogel L, Kroemer G. Immunogenic cell death modalities and their impact on cancer treatment. Apoptosis. 2009 Jan 15. [Epub ahead of print]
  19. Http://expasy.org/uniprot/P50580
  20. Wang S, Zheng Z, Weng Y, Yu Y, Zhang D, Fan W, Dai R, Hu Z. Angiogenesis and antiangiogenesis activity of Chinese medicinal herbal extracts. Life Sci, 2004;74(20):2467-2478 https://doi.org/10.1016/j.lfs.2003.03.005
  21. Cho WC. Application of proteomics in Chinese medicine research.Am J Chin Med. 2007;35(6):911-922 https://doi.org/10.1142/S0192415X07005375
  22. Chi CW, Chang YF, Chao TW, Chiang SH, P'eng FK, Lui WY, Liu TY. Flowcytometric analysis of the effect of berberine on the expression of glucocorticoid receptors in human hepatoma Hep G2 cells. Life Sci. 1994;54(26):2099-2107 https://doi.org/10.1016/0024-3205(94)00719-5
  23. Liu J, He C, Zhou K, Wang J, Kang JX. Coptis extracts enhance the anticancer effect of estrogen receptor antagonists on human breast cancer cells. Biochem Biophys Res Commun. 2009 Jan;378(2):174-178 https://doi.org/10.1016/j.bbrc.2008.10.169
  24. Walter S, Buchner J. Molecular chaperones cellular machines for protein folding. Angewandte Chemie (International ed. In English). 2002;41(7):1098-1113 https://doi.org/10.1002/1521-3773(20020402)41:7<1098::AID-ANIE1098>3.0.CO;2-9
  25. Shankardas J, Senchyna M, Dimitrijevich SD. Presence and distribution of 14-3-3 proteins in human ocular surface tissues. Mol Vis. 2008;14:2604-15. Epub 2008 Dec 31