DOI QR코드

DOI QR Code

Identification of Fel ursi and Cattle and Pig Bile Juices by speciesspecific PCR and PCR-RFLP

종 특이 PCR과 PCR-RFLP를 이용한 웅담과 기타 담류의 감별 방법

  • Kwon, Ki-Rok (College of Oriental Medicine, Sangji University) ;
  • Baek, Seung-Il (College of Oriental Medicine, Sangji University) ;
  • Choi, Suk-Ho (Division of Animal Science and Life Resources, Sangji University)
  • 권기록 (상지대학교 한의과대학 침구학교실) ;
  • 백승일 (상지대학교 한의과대학 침구학교실) ;
  • 최석호 (상지대학교 생명자원과학대학, 동물생명자원학부)
  • Published : 2009.03.30

Abstract

Objective : This study developed species-specific PCR and PCR-RFLP to detect the adulteration of Fel ursi products with cattle and pig bile juices. Methods : All the primers for PCR and PCR-RFLP in this study were designed based on nucleotide sequences of cytochrome b genes in the mitochondria. Results : The species-specific PCR amplified a DNA fragment of 214, 214, 295, and 167 bp from Fel ursi product, bear fur, cattle bile juice, and pig bile juice, respectively. The survey using the speciesspecific PCR indicated that some of commercial Fel ursi products were adulterated with cattle and pig bile juices. PCR-RFLP using the restriction endonucleases, HaeIII and HinfI enabled differentiation among Fel ursi product, cattle bile juice, and pig bile juice. Bear furs from two animals showed variations in PCR-RFLP patterns with HaeIII. Discussion : The detection methods of the species-specific PCR and PCR-RFLP could be useful in eliminating adulterated Fel ursi products from the market.

References

  1. 전국 한의과대학 본초학 교수 공저. 본초학. 영림사. 1998; 207-209, 224-226
  2. 김창민, 안덕균, 신민교, 이경순 등. 완역중약대사전 제7권. 정담. 1997; 2589-2593, 3289-3292
  3. 郭文, 陳德勇. 中藥熊膽製劑가 慢性乙型肝血淸 Hyaluronic acid에 대한 影響. 實用中醫藥雜誌. 1996; 3: 31
  4. 張恩勤. 中藥學. 上海中醫學院出版社. 1992: 331-332
  5. Jin ZG, Zhang TY, Liang WB & Liang CG. Effects of bear bile injection on blood lipid. Chinese Traditional and Herbal Drugs. 1997; 28: 4
  6. Kavamura T, Koiaumi F & Ishimori A. Effect of ursodeoxycholic acid on water immersion restraint stress ulcer of rats. J. Endocrinol. 1989; 55: 489 https://doi.org/10.1677/joe.0.0550489
  7. 남상천. 경락. 세명문화사. 1993; 324-328
  8. 대한약침학회. 약침요법조제지침서. 한성인쇄. 1999; 120-129
  9. Herman L. Determination of the animal origin of raw food by species-specific PCR. J Dairy Res 2001;68:429-35 https://doi.org/10.1017/S0022029901004940
  10. Lahiff S, Glennon M, O'Brien L, Lyng J, Smith T, Maher M, Shilton N. Species-specific PCR for the identification of ovine, porcine and chicken species in meta and bone meal (MBM). Mol Cell Probes 2001;15:27-35 https://doi.org/10.1006/mcpr.2000.0336
  11. Montiel-Sosa JF, Ruiz-Pesini E, Montoya J, Roncale's P, Lo'pez-Pe'rez MJ, Pe'rez-Martos A. Direct and highly species-specific detection of pork meat and fat in meat products by PCR amplification of mitochondrial DNA. J Agric Food Chem 2000;48:2829-32 https://doi.org/10.1021/jf9907438
  12. Meyer R, Hofelein C, Luthy J, Candrian U. Polymerae chain reaction-restriction fragment length polymorphism analysis: a simple method for species identification in food. J AOAC Int 1995;78:1542-51
  13. Partis L, Croan D, Guo Z, Clark T, Coldham T, Murby J. Evaluation of a DNA fingerprinting method for determining the species origin of meats. Meat Sci 2000;54:369-76 https://doi.org/10.1016/S0309-1740(99)00112-6
  14. Delisle I, Strobeck C. Conserved primers for rapid sequencing of the complete mitochondrial genome from carnivors applied to three species of bears. Mol Biol Evol 2002;19:357-61 https://doi.org/10.1093/oxfordjournals.molbev.a004090
  15. Hou W, Chen Y, Wu X, Hu J, Peng Z, Yang J, Tang Z, Zhou C, Li Y, Du Y, Kong L, Ren Z, Zhang H, Shuai S. A complete mitochondrial genome sequence of Asian black bear Sichuan subspecies (Ursus thibetanus mupinensis). Int J Biol Sci 2007;3:85-90 https://doi.org/10.1142/9789812708892_0004
  16. Acquaah G. Understading biotechnology an integrated and cyber-based approach. Pearson Prentice Hall 2004
  17. Dieffenbach CW, Dveksler GS ed. PCR primer a laboratory manual. Cold Spring Harbor 2003
  18. Zhang HY, Chen YD & Zhuang GL. Pharmacological action of bear bile extract in mice. Chinese Traditional and Herbal Drugs. 1996; 27: 10
  19. Janssen FW, Hagele GH, Buntjer JB, Lenstra JA. Species identification in meat by using PCR-generated satellite probes. J Ind Microbiol Biotech 1998;21:115-120 https://doi.org/10.1038/sj.jim.2900541
  20. Kingombe, CIB, Luthi E, Schlosser H, Howald D, Kuhn M, Jemmi T. A PCR-based test for specific determination of heat treatment conditions of animal meals as an effective prophylactic method for bovine spongiform encephalopathy. Meat Sci 2001;57:35-41 https://doi.org/10.1016/S0309-1740(00)00074-7
  21. Matsunaga T, Chikuni K, Tanabe R, Muroya S, Shibata K, Yamada J, Shinmura Y. A quick and simple method for the identification of meat species and meat products by PCR assay. Meat Sci 1999;51:143-148 https://doi.org/10.1016/S0309-1740(98)00112-0
  22. Bellagamba F, Moretti VM, Comincini S, Valfre F. 2001. Identification of species in animal feedstuffs by polymerase chain reactionrestriction fragment length polymorphism analysis of mitochondrial DNA. J Agric Food Chem 2001;49:3775-81 https://doi.org/10.1021/jf0010329