A rapid and easy fabrication of plant-tissue biosensor using rubber binder and its practicability test

고무 결합재를 이용한 식물조직 바이오센서의 간편한 제작과 응용성

  • Received : 2009.06.08
  • Accepted : 2009.08.25
  • Published : 2009.10.25

Abstract

An enzyme electrode bound by rubber solution was newly constructed and the test of its practicability were carried out. The binder of carbon powder was styrene-butadiene rubber dissolved in toluene and enzyme source was ground tissue of cabbage root. Volatilization of the solvent made the electrode material possess a mechanical robustness and a fast signal appearance. The electrode showed electrochemically irreversible characteristics and a powerful catalytic power (detection limit=$5.0{\times}10^{-5}M$, S/N=2). The double reciprocal plot of signal current and substrate concentration was ideally linear and the symmetry factor and exchange current density of the electrode used in this work were 0.35 and $4.93{\times}10^{-5}Acm^{-2}$ respectively.

Keywords

styrene-butadiene rubber;biosensor;peroxide;peroxidase;enzyme electrode

References

  1. I. R. W. Zwirtes de Oliveira and I. C. Vieira, Enzyme Microb. Technol. 38, 449-456(2006) https://doi.org/10.1016/j.enzmictec.2005.06.019
  2. T. A. Sergeyeva, N. V. Lavrik, A. E. Rachkov, Z. I. Kazantseva, S. A. Piletsky and A. V. El'skaya, Anal. Chim. Acta, 391, 289-297(1999) https://doi.org/10.1016/S0003-2670(99)00203-2
  3. K. J. Yoon, Anal. Sci. Tech. 20, 49-54(2007)
  4. S. Y. Choi, and K. J. Yoon, Elastomer, 41, 231-237(2006)
  5. A. N. Daz, M. C. R. Peinado and M. C. T. Minguez, Anal. Chim. Acta, 363, 221-227(1998) https://doi.org/10.1016/S0003-2670(98)00080-4
  6. J. Wang, H. Ye, Z. Jiang, N. Chen and J. Huang, Anal. Chim. Acta, 508, 171-176(2004) https://doi.org/10.1016/j.aca.2003.12.003
  7. V. Carralero Sanz, M. Luz Mena, A. Gonzlez-Corts, P. Yez-Sedeo and J. M. Pingarrn, Anal. Chim. Acta, 528, 1-8(2005) https://doi.org/10.1016/j.aca.2004.10.007
  8. X. Chen, J. Zhang, B. Wang, G. Cheng and S. Dong, Anal. Chim. Acta, 434, 255-260(2001) https://doi.org/10.1016/S0003-2670(01)00830-3
  9. A. Mansouri, D. P. M. Makris and P. Kefalas, J. Pham, Bio. Anal. 39, 22-26(2005) https://doi.org/10.1016/j.jpba.2005.03.044
  10. J. A. Brydson, 'Rubbery Materials and their Compounds', 99, Elsevier applied science publishers London and New York, 1988
  11. K. J. Yoon, Elastomer, 42, 112-118(2007)
  12. (a) K. J. Yoon, Anal. Sci. Tech. 16, 504-508(2003). (b) K. J. Yoon, Bull. Kor. Chem. Soc. 25, 997-1002(2004). (c) H. S. Kwon, E. H. Jin, K. J. Yoon and B. G. Lee, J. Kor. Chem. Soc. 49, 224-228(2005)
  13. Y. Miao and S. N. Tan, Anal. Chim. Acta, 437, 87(2001) https://doi.org/10.1016/S0003-2670(01)00986-2
  14. K. J. Yoon, Bull. Kor. Chem. Soc. 29, 2264-2266(2008) https://doi.org/10.5012/bkcs.2008.29.11.2264
  15. K. J. Yoon, J. Kor. Chem. Soc. 52, 197-202(2008) https://doi.org/10.5012/jkcs.2008.52.2.197