Chinese Medicine Granule Affects the Absorption and Transport of Glucose in Porcine Small Intestinal Brush Border Membrane Vesicles under Heat Stress

Song, Xiaozhen;Xu, Jianqin;Wang, Tian;Liu, Fenghua

  • Received : 2008.06.26
  • Accepted : 2008.09.22
  • Published : 2009.02.01


This study was conducted to investigate the effects of Chinese medicine granule (CMG, including Cortex Phellodendron, Atractylodes Rhizome, Agastache Rugosa and Gypsum Fibrosum) on absorption and transport of glucose in porcine small intestinal brush border membrane vesicles (BBMVs) under heat stress. Forty-eight 2-month-old Chinese experimental barrows were screened according to weight and litter origin, and then allotted to three groups and treated as follows: Normal temperature control group (NTCG; $23^{\circ}C$), high temperature control group (HTCG; $26^{\circ}C$ for 19 h, $40^{\circ}C$ for 5 h); Chinese medicine granule anti-stress group (CMGG; $26^{\circ}C$ for 19 h, $40^{\circ}C$ for 5 h) (n = 16 per group). The results showed that high temperature treatment decreased (p<0.05) the growth performance and intestinal glucose absorption but there was no change (p>0.05) in the expression of SGLT1 and GLUT2 genes in the small intestine of pigs compared with the NTCG. Dietary supplementation with CMG improved the growth performance, and increased the activity of disaccharidases in duodenum and jejunum of heat stressed pigs (p<0.05). CMG treatment increased (p<0.05) the protein levels of SGLT1 and GLUT2 in the small intestine, and up-regulated (p<0.05) the expression of SGLT1 and GLUT2 genes in the duodenum and jejunum but without changing (p>0.05) them in the ileum compared with the HTCG. These results indicated that CMG treatment significantly improved porcine growth performance, and increased intestinal glucose absorption and transport by BBMVs under heat stress, in addition to up-regulating the expression of SGLT1 and GLUT2 genes in porcine duodenum and jejunum.


Chinese Medicine Granule;Heat Stress;Pig;Intestinal BBMVs;Glucose Absorption


  1. Breves, G., J. Kock and B. Schröder. 2007, Transport of nutrients and electrolytes across the intestinal wall in pigs, Livest. Sci. 109:4-13
  2. Collin, A., J. V. Milgen and J. L. Dividich. 2001, The effect of high, constant temperature on food intake in young growing pigs, J. Anim. Sci. 72:519-527
  3. Jin, H. X. and J. S. Zhang. 2003, The effects of heat-stressresistant Chinese herbal activity on the performance of commercial pigs, Journal of Zhengzhou College of Animal Husbandry Engineering 23(3):165-166
  4. Liu, F. H., Z. H. Wang and B. Li. 2002, Influence of Chinese medicine additives on performance of growing-finishing pigs under heat stress, Feed Res. 4:1-4
  5. Nazifi, S., M. Saeb and E. Rowghani. 2003, The influences of thermal stress on serum biochemical parameters of Iranian fattailed sheep and their correlation with triiodothyronine (T3), thyroxine (T4) and cortisol concentrations, Comp. Clin. Path.12:135-139
  6. Patience, J. F., J. F. Umboh, R. K. Chaplin and C. M. Nyachoti. 2005, Nutritional and physiological responses of growing pigs exposed to a diurnal pattern of heat stress, Livest. Prod. Sci.96:205-214
  7. Sala-Rabanal, M., M. A. Gallardo, J. Sanchez and J. M. Planas. 2004, Na-dependent D-Glucose transport by intestinal brush border membrane vesicles from gilthead sea bream (sparus aurata), J. Membrane Bio. l 201:85-96
  8. Rodriguez, S. M., K. C. Guimaraes, J. C. Matthews, K. R. McLeod, R. L. Baldwin and D. L. Harmon. 2004, Influence of abomasal carbohydrates on small intestinal sodium-dependent glucose cotransporter activity and abundance in steers, J. Anim. Sci. 82(10):3015-3023
  9. Lu, Y. N., Q. Y. Qiu and Y. Wang. 2006, Effects of phellodendron and its main components on the cell membrane fluidity, Chinese J. Pathophysiol. 22(1):156-159
  10. Spencer, J. D., A. M. Gained, E. P. Berg and G. L. Allee. 2005, Diet modification to improve finishing pig growth performance and pork quality attributes during periods of heat stress, J. Anim. Sci. 83:243-254
  11. Dong, H. 2008. Effects of traditional Chinese medicine on gut hormones in pig and rat under high temperature. Ph.D. Thesis. China agriculture university. Beijing. China
  12. Song, X. Z., L. Lu, F. H. Liu, L. Zhang and T. Wang. 2008, Effect of high temperature stress on lipid peroxidation of small intestinal epithelium in pigs, Acta Zoonutrimenta Sinica 20(1):75-79
  13. Sutherland, M. A., S. R. Niekamp, S. L. Rodriguez-Zas and J. L. Salak-Johnson. 2006, Impacts of chronic stress and social status on various physiological and performance measures in pigs of different breeds, J. Anim. Sci. 84(3):588-596
  14. Huynh, T. T. T., A. J. A. Aarnink and M. W. A. Verstegent. 2005, Effects of increasing temperatures on physio-logical changes in pigs at different relative humidities, J. Anim. Sci. 83(2):1385-1396
  15. Hou, Y. Q., Y. L. Liu, J. Hu and W. H. Shen. 2006, Effects of lactitol and tributyrin on growth performance, small intestinal morphology and enzyme activity in weaned pigs, Asian-Aust. J. Anim. Sci. 19(10):1470-1477
  16. Chen, X. X., B. He, X. Q. Li, H. Y. Li and J. P. Luo. 1998, Comparison of effects of three extracts of herba pogostemonis on the intestinal function, Pharmacology and Clinics of Chinese Materia Medica 14(2):31-33
  17. Kong, X. F., G. Y. Wu, Y. P. Liao, Z. P. Hou, H. J. Liu, F. G. Yin, T. J. Li, R. L. Huang, Y. M. Zhang and D. Deng. 2007, Effects of Chinese herbal ultra-fine powder as a dietary additive on growth performance, serum metabolites and intestinal health in early-weaned piglets, Livest. Sci. 108:272-275
  18. Garriga, C., M. Moreto and J. M. Planas. 2000, Effects of resalination on intestinal glucose transport in chickens adapted to low Na+ intakes. Experimental Physiology 85(4):371-378
  19. Nonaka, I., N. Takusari, K. Tajima, T. Suzuki, K. Higuchi and M. Kurihara. 2008, Effects of high environmental temperatures on physiological and nutritional status of prepubertal Holstein heifers, Livest. Sci. 113:14-23
  20. Khajavi, M., S. Rahimi, Z. M. Hassan, M. A. Kamali and T. Mousavi. 2003, Effect of feed restriction early in life on humoral and cellular immunity of two commercial broiler strains under heat stress conditions. Br, Poult. Sci. 44:490-497
  21. Hu, Y. X., R. P. She and H. Y. Zhang. 2006, Studies on the dynamic changes of the level of IL-2, IFN-$\gamma$ and TNF-$\propto$ in porcine serum after heat stress, Acta Veterinaria and Zootechnica Sinica 37(5):496-499
  22. Chen, Y. M., J. Chen and G. X. Chou. 2006, Research review on chemical constituent and pharmacological action of rhizoma atractylodis, Acta Universitatis Traditionis Medicalis Sinensis Pharmacologiaeque Shanghai 20(4):95-98
  23. Schulthess, G., S. Compassi, D. Boffelli, M. Werder, F. E. Weber, and H. Hauser. 1996, A comparative study of sterol absorption in different small-intestinal brush border membrane models, J. Lipid Res. 37:2405-2420
  24. Jane, D., V. Steven, P. Timothy, S. P. King and B. Shirazi. 2003, Glucose sensing in the intestinal epithelium, Eur. J. Biochem. 270:3377-3388
  25. Hu, Y. X., R. P. She and H. Y. Zhang. 2006, Studies on the dynamic changes of the level of IL-2, IFN-γ and TNF-$\propto$ in porcine serum after heat stress, Acta Veterinaria and Zootechnica Sinica 37(5):496-499
  26. Wang, Z. L., T. Q. Yu, X. Y. Zhu, H. Y. Chen, F. H. Liu and J. Q. Xu. 2007, Effects of Chinese medicine granule on concentration of IL-2, IL-10 and IgA in porcine intestine after heat stress, J. Chinese Vet. Med. 9:12-15
  27. Hyun, Y., M. Ellis, G. Riskowski and R. W. Johnson. 1998, Growth performance of pigs subjected to multiple concurrent environmental stressors, J. Anim. Sci. 76:721-727
  28. Nabuurs, M. J. A., G. J. van Essen, P. Nabuurs, T. A. Niewold and M. J. vander. 2001, Thirty minutes transport causes small intestinal acidosis in pigs, Res. Vet. Sci. 70:123-127
  29. Xu, Z. R., W. F. Li and J. Y. Sun. 2002, Properties of gastrointestinal disaccharidase in pig, Acta Zoologica Sinia 48(2):202-207
  30. Dahlqvist, A. 1961, The location of carbohydrases in the digestive tract of the pig, Biochem. J. 78(2):282-288

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