Isolation of Photobacterium damselae subsp. damselae from zebra shark Stegostoma fasciatum

  • Han, Jee Eun (College of Veterinary Medicine, Seoul National University) ;
  • Gomez, Dennis K. (College of Veterinary Medicine, Seoul National University) ;
  • Kim, Ji Hyung (College of Veterinary Medicine, Seoul National University) ;
  • Choresca, Casiano H. Jr. (College of Veterinary Medicine, Seoul National University) ;
  • Shin, Sang Phil (College of Veterinary Medicine, Seoul National University) ;
  • Baeck, Gun Wook (Institute of Marine Industry, Marine Bio Education & Research Center, College of Marine Science, Gyeongsang National University) ;
  • Park, Se Chang (College of Veterinary Medicine, Seoul National University)
  • Accepted : 2009.03.19
  • Published : 2009.03.30

Abstract

The zebra shark Stegostoma fasciatum which had been reared in the commercial aquaria was found dead and submitted for postmortem examination. A pure bacterial culture was isolated from pale and enlarged liver. The analysis of ureC and 16S rRNA genes confirmed the isolate as Photobacterium (P.) damselae subsp. damselae and this pathogen was sensitive to gentamicin. Although, no mortality in mouse was observed in the experimental infection study, the isolation of this pathogen in aquarium fish is significant because it can act as a reservoir to other aquatic animals and can also be zoonotic potential to human during aquarium management. This paper describes the first isolation of P. damselae subsp. damselae from zebra shark.

Keywords

Acknowledgement

Supported by : Korea Research Foundation

References

  1. Austin B, Austin DA. Vibrionaceae representatives. In:Laird LM (ed.). Bacterial Fish Pathogens. 3rd ed. pp.265-294, Ellis Horwood, Chichester, 1993
  2. Balebona MC, Andreu MJ, Bordas MA, Zorrilla I,Morinigo MA, Borrego JJ. Pathogenicity of Vibrioalginolyticus for cultured gilt-head sea bream (Sparusaurata L.). Appl Environ Microbiol 1998, 64, 4269-4275
  3. Bergey DH, Holt JG. Bergey's Manual of Determinative Bacteriology. 9th ed. pp. 192-194, Williams & Wilkins, Baltimore, 1994
  4. Clarridge JE, Zighelboim-Daum S. Isolation andcharacterization of two hemolytic phenotypes of Vibriodamsela associated with a fatal wound infection. J ClinMicrobiol 1985, 21, 302-306
  5. Dudgeon CL, Feldheim K, Schick M, Ovenden JR.Polymorphic microsatellite loci for the zebra sharkStegostoma fasciatum. Mol Ecol Notes 2006, 6, 1086-1088
  6. Fouz B, Larsen JL, Nielsen B, Barja JL, ToranzoAE. Characterization of Vibrio damsela strains isolatedfrom turbot Scophthalmus maximus in Spain. Dis Aquat Organ 1992, 12, 155-166 https://doi.org/10.3354/dao012155
  7. Fouz B, Toranzo AE, Marco-Noales E, Amaro C.Survival of fish-virulent strains of Photobacteriumdamselae subsp. damselae: in seawater under starvationconditions. FEMS Microbiol Lett 1998, 168, 181-186 https://doi.org/10.1111/j.1574-6968.1998.tb13271.x
  8. Fouz B, Toranzo AE, Milan M, Amaro C. Evidencethat water transmits the disease caused by the fishpathogen Photobacterium damselae subsp. damselae. JAppl Microbiol 2000, 88, 531-535 https://doi.org/10.1046/j.1365-2672.2000.00992.x
  9. Gauthier G, Lafay B, Ruimy R, Breittmayer V,Nicolas JL, Gauthier M, Christen R. Small-subunitrRNA sequences and whole DNA relatedness concurfor the reassignment of Pasteurella piscicida (Snieszkoet al.) Janssen and Surgalla to the genus Photobacteriumas Photobacterium damsela subsp. piscicidacomb. nov. Int J Syst Bacteriol 1995, 45, 139-144
  10. Kothary MH, Kreger AS. Purification and characterizationof an extracellular cytolysin produced by Vibrio damsela. Infect Immun 1985, 49, 25-31
  11. Love M, Teebken-Fisher D, Hose JE, Farmer JJ 3rd, Hickman FW, Fanning GR. Vibrio damsela, amarine bacterium, causes skin ulcers on the damselfishChromis punctipinnis. Science 1981, 214, 1139-1140 https://doi.org/10.1126/science.214.4525.1139
  12. Magarinos B, Toranzo AE, Romalde JL. Phenotypicand pathobiological characteristics of Pasteurellapiscicida. Annu Rev Fish Dis 1996, 6, 41-64 https://doi.org/10.1016/S0959-8030(96)90005-8
  13. Osorio CR, Toranzo AE, Romalde JL, Barja JL. Multiplex PCR assay for ureC and 16S rRNA genesclearly discriminates between both subspecies of Photobacterium damselae. Dis Aquat Organ 2000, 40,177-183 https://doi.org/10.3354/dao040177
  14. Smith SK, Sutton DC, Fuerst JA, Reichelt JL.Evaluation of the genus Listonella and reassignment ofListonella damsela (Love et al.) MacDonell andColwell to the genus Photobacterium as Photobacteriumdamsela comb. nov. with an emendeddescription. Int J Syst Bacteriol 1991, 41, 529-534 https://doi.org/10.1099/00207713-41-4-529