Chip-based isothermal amplification method for EGFR gene mutations in lung cancer

칩 기반 등온 증폭반응법을 이용한 폐암에서의 EGFR 유전자 돌연변이 검출 시스템 개발

  • Ahn, Young-Chang (Department of Chemistry, School of Advanced Science and Basic Science Research Institute, Dankook University) ;
  • Park, Su-Min (Department of Chemistry, School of Advanced Science and Basic Science Research Institute, Dankook University) ;
  • Seo, Jae-Won (Department of Chemistry, School of Advanced Science and Basic Science Research Institute, Dankook University) ;
  • Yoon, Il-Kyu (Department of Chemistry, School of Advanced Science and Basic Science Research Institute, Dankook University) ;
  • Jung, Duck-Hyun (Department of Chemistry, School of Advanced Science and Basic Science Research Institute, Dankook University) ;
  • Lee, Eun-Young (Department of Chemistry, School of Advanced Science and Basic Science Research Institute, Dankook University) ;
  • Nam, Youn-Hyoung (Department of Chemistry, School of Advanced Science and Basic Science Research Institute, Dankook University) ;
  • Jang, Won-Cheoul (Department of Chemistry, School of Advanced Science and Basic Science Research Institute, Dankook University) ;
  • Seung, Kwon Pil (Department of Laboratory Medicine, Dankook University Hospital) ;
  • Kim, Jong-Wan (Department of Laboratory Medicine, Dankook University College of Medicine)
  • 안영창 (단국대학교 첨단과학대학 화학과) ;
  • 박수민 (단국대학교 첨단과학대학 화학과) ;
  • 서재원 (단국대학교 첨단과학대학 화학과) ;
  • 윤일규 (단국대학교 첨단과학대학 화학과) ;
  • 정덕현 (단국대학교 첨단과학대학 화학과) ;
  • 이은영 (단국대학교 첨단과학대학 화학과) ;
  • 남윤형 (단국대학교 첨단과학대학 화학과) ;
  • 장원철 (단국대학교 첨단과학대학 화학과) ;
  • 권필승 (단국대학교병원 진단검사의학과) ;
  • 김종완 (단국대학교 의과대학 진단검사 의학교실)
  • Received : 2009.04.30
  • Accepted : 2009.10.22
  • Published : 2009.12.25

Abstract

Lung cancer is the main cancer on the world today, due to the high case fatality. Lung cancer can devide into two major types, such as small-cell lung cancer (SCLC) and non-small-cell lung cancer (NSCLC). Mutations in the epidermal growth factor receptor (EGFR) have been described in patients with advanced NSCLC. Mutations in the EGFR are associated with clinical and radiographic responses to EGFR tyrosine kinase inhibitors gefitinib and erlotinib. Thus, the detection of EGFR mutation can offer an effective information in clinical decision-making. In this study, We developed very simple, cheep and rapid mutation detection system by chip-based isothermal amplification method. The method described here has shown the advantages of rapid amplification, high sensitivity, and specificity. Also, it will be useful for rapid and reliable clinical diagnosis of EGFR mutation.

Keywords

Chip;EGFR (Epidermal Growth Factor Receptor);Isothermal Amplification;Non-Small-Cell Lung Cancer (NSCLC)

Acknowledgement

Supported by : 한국산업기술진흥원

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