Comparison of Anthocyanin Content in Seed Coats of Black Soybean [Glycine max(L.) Merr.] Cultivars Using Liquid Chromatography Coupled to Tandem Mass Spectrometry

  • Shin, Sung-Chul (Department of Chemistry and Research Institute of Life Science, Gyeongsang National University) ;
  • Lee, Soo-Jung (Department of Food and Nutrition, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Lee, Sung-Joong (Department of Chemistry and Research Institute of Life Science, Gyeongsang National University) ;
  • Chung, Jong-Il (Department of Agriculture and Life Science Research Institute, Gyeongsang National University) ;
  • Bae, Dong-Won (Central Laboratory, Gyeongsang National University) ;
  • Kim, Soo-Taek (Department of Informational Statistics and Research Institute of Natural Science, Gyeongsang National University) ;
  • Sung, Nak-Ju (Department of Food and Nutrition, Institute of Agriculture and Life Science, Gyeongsang National University)
  • Published : 2009.12.31

Abstract

The seed coat of the black soybean contains 3 main anthocyanins such as delphinidin-3-O-$\beta$-glucoside, cyanidin-3-O-$\beta$-glucoside, and petunidin-3-O-$\beta$-glucoside. As a part of our effort on discovering and breeding new black soybean cultivars which possesses specific anthocyanin component rich, we determined the anthocyanin profiles of the 2 cultivars recently developed soybean cv. Gaechuck #1 and cv. Gyeongsang #1, using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and compared their content and identity with those of previously known 10 cultivar controls. The Cosmosil-$5C_{18}$-AR-II column were selected for the analysis because of the best peak separation. The column temperature was set up at $35^{\circ}C$. The mobile phase consisting of water containing 0.5%(v/v) formic acid and methanol gave good separation between the 3 anthocyanin analytes and internal standard (quercetin 3-O-$\beta$-rutinoside) and peaks with suppressed tail. The MS/MS spectra of each individual anthocyanin standard were detected in positive electron spray ionization (ESI) modes. It was disclosed that the anthocyanin contents of the soybean cv. Gaechuck#1 and cv. Gyeongsang#1 are roughly higher than those of the 10 controls.

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