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Isolation of Bacteria from Chunggukjang Prepared by Rice Straw and Identification of Protease Secreted

볏짚 청국장 발효 세균 분리 및 분비된 protease의 확인

  • Oh, Jae-Hyeon (Department of Biological Sciences, Sunchon National University) ;
  • Lee, Byeong-Jeong (Department of Biological Sciences, Sunchon National University) ;
  • Paik, Hyoung-Rok (Department of Biological Sciences, Sunchon National University) ;
  • Jung, Sang-Chul (Department of Biological Sciences, Sunchon National University) ;
  • Baik, Keun-Sik (Department of Biological Sciences, Sunchon National University) ;
  • Choi, Sang-Ki (Department of Biological Sciences, Sunchon National University)
  • Published : 2009.03.31

Abstract

To isolate bacteria secreting protease, which can dissolve fibrin efficiently, we prepared chunggukjang using rice straw and isolated, preliminarily, approximately 100 bacterial stains. Their capabilities to dissolve milk protein as well as fibrin included in media were then examined and finally, five strains named J1 - J5 were selected. Among them, J-4, which is close to bacillus subtilis, showed highest activity for fibrin dissolution. Proteases secreted from the J-4 strain were partially purified from culture supernatant using DEAE-sepharose column chromatography and identified with SDS-polyacrylamide gel electrophoresis. Three proteins were subjected to analysis with MALDI-TOF and PMF (Peptide Mass Fingerprinting). 41.9 kDa protein was identified as a neutral protease. On the other hand, 45 kDa protein turned out to be bacillopeptidase F, with a molecular mass of 91.7 kDa, indicating that partially purified peptide is a degradation product.

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