Antioxidative Activity of Pine (Pinus densiflora) Needle Extracts in Rats Fed High-Cholesterol Diet

고콜레스테롤 식이 섭취 흰쥐에 대한 적송잎 추출물의 항산화 효과

  • Published : 2009.04.30


This study was conducted to investigate the effects of pine (Pinus densiflora) needle extracts on hepatic antioxidative system in rats fed high-cholesterol diet. Eight-week old Sprague Dawley rats were divided into four groups; normal diet group (CON), 0.5% cholesterol diet group (0.5% CHOL), 0.5% cholesterol diet and pine needle extracts prepared by using hot water group (HOT water), and 0.5% cholesterol diet and pine needle extracts prepared by using sub-supercritical $CO_2$ group (Sub-supercritical). The serum ALP, AST, and ALT activities were increased by 0.5% cholesterol diet supplementation, but treatment with pine needle extracts showed decrease compared with 0.5% CHOL group. The SOD activity in liver at 0.5% cholesterol diet group was more decreased than control group while Sub-supercritical group had significantly higher SOD activity than 0.5% CHOL group. Moreover, groups with pine needle extracts supplementation had higher level of GPx in liver than 0.5% cholesterol diet group. These results suggest that pine needle extracts increased antioxidative activities.


  1. Trackshel G, Maines MD. 1988. Characterization of glutathione S-transferase in rat kidney. Biochem J 252: 127-136
  2. Simic MG. 1988. Mechanisms of inhibition of free radical processed in mutagenesis and carcinogenesis. Mutat Res 202: 386-399
  3. Lin MY, Yen CL. 1999. Inhibition of lipid peroxidation by Lactobacillus acidophilus and Bifidibacterium longum. J Agric Food Chem 47: 3661-3664
  4. Ito M, Ohishi K, Yoshida Y, Yokoi W, Sawada H. 2003. Antioxidative effects of lactic and bacteria on the colonic mucosa of iron-overloaded mice. J Agric Food Chem 51: 4456-4460
  5. Wursch P. 1979. Influence of tannin-rich carob pod fiber on the cholesterol metabolism in the rat. J Nutr 109: 685-692
  6. Yoon SO, Yoo SJ, Oh S. 1997. A study on the pharmacological and nutritional values of needles of Pinus densiflora S. Research Bulletin of the Experiment Foresta 5: 63-81
  7. Plaa GL, Charbonneau M. 1994. Detection and evaluation of chemically induced liver injury. In Principles and methods of toxicology. Hayes AW, ed. Taylor & Francis, Philadelphia, USA. p 839-870
  8. Umemura T, Kodama Y, Nishikawa A, Nomura T, Kanki K, Kuroiwa Y, Ishii Y, Kurokawa Y, Hirosa M. 2006. Nine-week detection of six genotoxic lung carcinogens using the rasH2/BHT mouse model. Cancer Lett 231: 314-318
  9. Cort WM. 1984. Antioxidant activity of tocopherols and ascorbyl palmitate and ascorbic acid and their mode of action. J Am Oil Chem Soc 51: 321-325
  10. Hong TG, Lee YL, Im MH, Hong JN. 2004. Studies on the physiological functionality and antimicrobial activity of pine needle fermentation extract. Korean J Food Preserv 11: 94-99
  11. Mantha SV, Kalra J, Prasad K. 1996. Effects of probucol on hypercholesterolemia-induced changes in antioxidant enzymes. Life Sci 58: 503-509
  12. Jeon SM, Bok SH, Jang MK, Lee MK, Nam KT, Park YB, Rhee SJ, Choi MS. 2001. Antioxidative activity of naringin and lovastatin in high cholesterol-fed rabbits. Life Sci 69: 2855-2866
  13. Pryor WA. 1977. Involvement of radical reaction in aging and carcinogenesis in medicine chemistry. In Free radical in biology. Elservier, Amsterdam. p 331-361
  14. Hur SK, Kim SS, Heo YH, Ahn SM, Lee BG, Lee SK. 2001. Effect of the grapevine shoot extract on free radical scavenger activity and inhibition of pro-inflammatory mediator production in raw macrophages. J Appl Pharmacol 9: 188-193
  15. Jae JU. 1989. Isolation and identification of constituents from antioxidant and hematopoietic fractions of Panax gineseng C.A. Mayer. PhD Dissertation. Seoul University, Seoul
  16. Sang DJ, Kim H, Hoon IO. 1981. Antioxidant activity of panax ginseng browing products. J Korean Agric Chem Soc 24: 161-166
  17. Lawrence RA, Burk RF. 1976. Glutathione peroxidase activity in selenium-deficient rat liver. Biochem Biophys Res Commum 71: 952-958
  18. Lee OH, Kim KY, Jang MK, Yu KH, Kim SG, Kim MH, Lee SH. 2008. Evaluation of proanthocyanidin contents in total polyphenolic compounds of pine (Pinus densiflora) needle extracts and their antioxidative activities. Korea J Life Sci 18: 213-219
  19. Lowry OH, Rosenbrough NJ, Farr AS, Randall RJ. 1951. Protein measurement with Folin phenol reagent. J Biol Chem 193: 256-261
  20. Fridovich I. 1986. Biologic effects of the superoxide radicals. Arch Biochem Biophysm 247: 1-15
  21. Aebi H. 1984. Catalase in vitro methods. Enzymology 105: 121-126
  22. Ahmed AE. 1979. Activities of enzymes of the pancreas and the lumens and mucosa of the small in growing broiler cockerels for on tannin-containing diet. J Nutr 65: 189-192
  23. Barry TN, Manley TR, Duncan SJ. 1986. The role of condensed tannins in the nutritional value of Lotus pedunculatus for sheep. Br J Nutr 55: 123-128
  24. Dodge JA. 1994. Dietary fats and gastrointestinal function. Eur J Clin Nutr 48: S8-16
  25. Mehansho H, Hagerman A, Clernents S, Butler L, Rogler J, Mcarlson D. 1983. Dualation of proline-rich peotin biosynthesis in rat paroids glands by sorghum with high tannin levels. Proc Natl Acad Sci USA 80: 3948-3956
  26. Martijn BK, Peter LZ, Ronald PM. 1994. Effect of fats and fatty acids on blood lipid in humans: an overview. Am J Clin Nutr 60: 1017S
  27. Shin CS. 2006. Effect of powder of small water dropwort (Oenanthe javanica DC) and brewer's yeast (Saccharomyces cerevisiae) on the liver function and serum lipid metabolism in alcohol-consumed rats. MS Thesis. Chung-Ang University, Chungnam
  28. Lim SS, Jung HO, Jung BM. 1997. Effect of Ixeris sonchifolia H. on serum lipid metabolism in hyperlipidemic rats. Korean J Nutr 30: 889-894
  29. Sung NJ, Lee SJ, Shin JH, Chung MJ, Lim SS. 1998. Effects of Houttuynia cordata Thunb powder and juice on lipid consumption of liver, brain and kidney in dietary hypercholesterolemic rats. J Korean Soc Food Sci Nutr 27: 1230-1235
  30. Kwon MJ, Song YS, Song YO. 2003. Antioxidative effect of kimchi ingredients on rabbits fed cholesterol diet. J Korean Soc Food Sci Nutr 27: 1189-1196
  31. Chung EJ, Um YS, Nam HW, Park T. 2003. Changes in lipid peroxidation level and antioxidant enzyme activities of rats supplemented with dietary cholesterol and/or taurine. J Korean Soc Food Sci Nutr 32: 1310-1317
  32. Kim KR, Choi JH, Lee SK, Woo MH, Choi SW. 2006. Effect of enzymatic hydrolysate of hamcho (Salicornia herbacea) on antioxidative defense system in rats fed high cholesterol diet. J Korean Soc Food Sci Nutr 35: 1356-1362
  33. Lee JJ, Choi MH, Lee MY. 2006. Effect of Pimpinella brachycarpa extract on lipid metabolism in rats fed high cholesterol diet. J Korean Soc Food Sci Nutr 35: 1151-1158
  34. Seo JA. 1997. Effects of plasma cholesterol metabolism and organization change of rabbit high cholesterol diet. MS Thesis. Kyungpook National University, Daegu
  35. Lim SS, Kim MH, Lee JH. 1997. Effect of Artemisia princeps var orientalis and circium japonicum var ussuriense on liver function body lipid and bile acid of hyperlipidemic rat. Korean J Nutr 30: 797-802
  36. Kang YH, Park YK, Ha TY, Moon KD. 1996. Effects of pine needle extracts on enzyme activities of serum and liver and liver morphology in rats fed high fat diet. J Korean Soc Food Nutr 25: 374-378
  37. Freeman BA, Crapo JD. 1982. Biology of disease free radicals and tissue injury. Lab Inves 47: 412-236
  38. Tappel AL. 1980. Vitamin E and selenium protection from in vivo lipid peroxidation. Ana New York Sa 355: 18-24
  39. Kang YH, Park YK, Oh SR, Moon KD. 1995. Studies on the physiological functionality of pine needle and muguort extract. Korea J Food Sci Technol 27: 978-984
  40. Johansson LH, Borg LA. 1988. A spectrophotometric method for determination of catalase activity in small tissue samples. Anal Biochem 174: 331-336
  41. Vergmeyer HU, Aebi H. 1974. Catalase in methods of enzymatic analysis. Academic press, New York. Vol 2, p 673-684
  42. Jones DP, Eklow L, Orrenius S. 1981. Metabolism of hydrogen peroxide in isolated hepatocytes: relative contribution of catalase and glutathione peroxidase in decomposition of endogenously generated $H_2O_2$. Arch Biochem Biophys 210: 505-516
  43. Meister A. 1994. Glutathione-ascorbic acid antioxidant system in animals. J Biol Chem 269: 9337-9400

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