Effects of Fermented Turmeric (Curcuma longa) by Bacillus natto Supplementation on Liver Function and Serum Lipid Parameters in Mice

낫토균으로 발효한 발효울금의 투여가 마우스의 간 기능 및 혈중 지질 함량에 미치는 영향

  • Kang, Jae-Ku (Myunggok Medical Research Institute, College of Medicine, Konyang University) ;
  • Kang, Hyo-Jin (Myunggok Medical Research Institute, College of Medicine, Konyang University) ;
  • Seo, Ji-Hye (Myunggok Medical Research Institute, College of Medicine, Konyang University) ;
  • Kim, Sun-Ok (Myunggok Medical Research Institute, College of Medicine, Konyang University) ;
  • Choi, Jung-Hyo (Myunggok Medical Research Institute, College of Medicine, Konyang University) ;
  • Cho, Do-Yeun (Myunggok Medical Research Institute, College of Medicine, Konyang University) ;
  • Park, Chang-Gyo (Myunggok Medical Research Institute, College of Medicine, Konyang University) ;
  • Lee, Hoi-Young (Myunggok Medical Research Institute, College of Medicine, Konyang University)
  • 강재구 (건양대학교 의과대학 명곡의과학연구센타) ;
  • 강효진 (건양대학교 의과대학 명곡의과학연구센타) ;
  • 서지혜 (건양대학교 의과대학 명곡의과학연구센타) ;
  • 김선옥 (건양대학교 의과대학 명곡의과학연구센타) ;
  • 최정효 (건양대학교 의과대학 명곡의과학연구센타) ;
  • 조도연 (건양대학교 의과대학 명곡의과학연구센타) ;
  • 박창교 (건양대학교 의과대학 명곡의과학연구센타) ;
  • 이회영 (건양대학교 의과대학 명곡의과학연구센타)
  • Published : 2009.04.30


The effects of turmeric and fermented turmeric by Bacillus natto on antioxidant activities, liver function recovery of acute hepatotoxicity mice, and serum lipid parameters in high fat diet fed mice were investigated. In the results of antioxidant activity by DPPH method, fermented turmeric had higher antioxidative activity than turmeric. Acute hepatotoxicity was induced by 0.5 mL of carbon tetrachloride ($CCl_4$) per kg of mice. Unlike turmeric, fermented turmeric significantly reduced the levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) after 5 days compared to the controls with 0.5% methyl cellulose (p<0.05). In addition, higher recovery of liver damage by $CCl_4$ was observed in mice with fermented turmeric than with turmeric. High fat (20%) diet fed mice were divided into 4 groups to investigate the effects of turmeric and fermented turmeric on serum lipid parameters: C (vehicle), TuL (low dose (80 mg/kg) with turmeric), TuH (high dose (160 mg/kg) with turmeric), FTuL (low dose with fermented turmeric), and FTuH (high dose with fermented turmeric). The levels of LDL-cholesterol and HDL-cholesterol were significantly reduced and increased in FTuL, FTuH and TuH groups compared to the C group, respectively. However, there was no significant change in triglyceride levels by either turmeric or fermented turmeric compared to those by control. Collectively, these results strongly suggest that fermented turmeric by Bacillus natto could be used as a functional food for enhancement of health with better consumer acceptance.


  1. Kim CR. 2006. Enhancement of liver function by curcuma extract on acute hepatotoxicity in rat. Korean J Food Sci Ani Resour 26: 386-393
  2. Chi HJ, Kim HS. 1983. Curcumin content of cultivated tumeric in Korea. Korean J Pharmacognosy 14: 67-69
  3. Arora RB, Basu N, Kapoor V, Jain AP. 1971. Anti-inflammatory studies on Curcuma longa (turmeric). Indian J Med Res 59: 1289-1295
  4. Chainani WN. 2003. Safety and anti-inflammatory activity of curcumin: a component of turmeric (Curcuma longa). J Altern Complement Med 9: 161-168
  5. Aggarwal BB, Kumar A, Bhartic AC. 2003. Anticancer potential of curcumin: preclinical and clinical studies. Anticancer Res 23: 363-398
  6. Huang MT, Smart RC, Wong CQ, Conney AH. 1988. Inhibitory effect of curcumin, chlorogenic acid, caffeic acid and ferulic acid on tumor promotion in mouse skin by 12-O-tetradecanoylphorbol-13-acetate. Cancer Res 48: 5941-5946
  7. Ozaki K, Kawata Y, Amano S, Hanazawa S. 2000. Stimulatory effect of curcumin on osteoclast apoptosis. Biochem Pharmacol 52: 437-440
  8. Ferreira LAF, Henriques OB, Andreoni AAS, Vital GRF, Campos MMC, Habermehl GG, Moraes VLG. 1992. Antivenom and biological effects of ar-turmerone isolated from Curcuma longa (Zingiberaceae). Toxicon 30: 1211-1218
  9. Unnikrishnan MK, Rao MN. 1995. Inhibition of nitrite induced oxidation of hemoglobin by curcuminoids. Pharmazie 50: 490-492
  10. Park EJ, Yoon KR, Kim JH, Kang WS. 1998. Antioxidative property of turmeric (curcumae rhizoma) ethanol extract. Korean J Food Sci Technol 30: 266-271
  11. Deshpande UR, Gadre SG, Raste AS, Pillai D, Bhide SV, Samuel AM. 1998. Protective effect of turmeric (Curcuma longa L) extract on carbon tetrachloride-induced liver damage in rat. Indian J Exp Biol 36: 573-577
  12. Park EJ, Jeon CH, Ko G, Kim J, Sohn DH. 2000. Protective effect of curcumin in rat liver injury induced by carbon tetrachloride. J Pharm Pharmacol 52: 437-440
  13. Park KJ. 2006. Method for fermenting tumeric and method for making eatable composition having main material for tumeric. Korean Patent 565,989
  14. Kim MO, Oh SC, Kim SS. 2008. Method of manufacturing curcuma domestica fermented extract. Korean Patent 842,022
  15. Milner M, Makise K. 2002. Natto and its active ingredient nattokinase: A potent and safe thrombolytic agent. Alternative & Complementary Therapies 8: 157-164
  16. Nam HK. 1987. Influence of magnesium and calcium on the serum cholesterol level lowering (III)-Influence of Korea ginseng. J Korean Soc Food Nutr 16: 18-21
  17. Kwon OJ, Choi UK, Lee EJ, Cho YJ, Cha WS, Son DH, Chung YG. 2000. Chemical changes of meju made with barly bran using fermentation. Korean J Food Sci Technol 32: 1135-1141
  18. Ju JC, Shin JH, Lee SJ, Cho HS, Sung NJ. 2006. Antioxidative activity of hot water extracts from medicinal plants. J Korean Soc Food Sci Nutr 35: 7-14
  19. Sharma RA, McLelland HR, Hill KA, Ireson CR, Euden SA, Manson MM, Pirmohamed M, Marnett LJ, Gescher AJ, Steward WP. 2001. Pharmacodynamic and pharmacokinetic study of oral curcuma extract in patients with colorectal cancer. Clin Cancer Research 7: 1894-1900
  20. Pan MH, Huang TM, Lin JK. 1999. Biotransformation of curcumin through reduction and glucuronidation in mice. Drug Metab Dispos 27: 486-494
  21. Kim MH, Lee YS, Lee DH, Park HS, Sung CJ. 2001. The study of relation among serum copper, zinc, leptin and lipids of middle-school girls. J Korean Soc Food Sci Nutr 30: 540-546
  22. Jun YS, Sung CJ. 1996. Effects of dietary Fe and Se levels on lipid levels in serum and liver of rats. J Korean Soc Food Sci Nutr 25: 568-574
  23. Kim TH, Son YK, Hwang KH, Kim MH. 2008. Effects of Angelica keiskei Koidzumi and turmeric extract supplementation on serum lipid parameters in hypercholesterolemic diet or P-407-induced hyperlipidemic rats. J Korean Soc Food Sci Nutr 37: 708-713

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