Induction of Apoptosis in HT-29 Human Colon Cancer Cells by the Pepper Component Piperine

후추의 주요 성분인 Piperine의 대장암세포 세포사멸 유도 효과

  • Kim, Eun-Ji (Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University) ;
  • Park, Hee-Sook (Dept. of Food Science and Nutrition, Hallym University) ;
  • Shin, Min-Jeong (Dept. of Food Science and Nutrition, Hallym University) ;
  • Shin, Hyun-Kyung (Dept. of Food Science and Nutrition, Hallym University) ;
  • YoonPark, Jung-Han (Dept. of Food Science and Nutrition, Hallym University)
  • 김은지 (한림대학교 식의약품의 효능평가 및 기능성소재개발센터) ;
  • 박희숙 (한림대학교 식품영양학과) ;
  • 신민정 (한림대학교 식품영양학과) ;
  • 신현경 (한림대학교 식품영양학과) ;
  • 윤정한 (한림대학교 식품영양학과)
  • Published : 2009.04.30


Piperine is an alkaloid-amine found in pepper and has been reported to have anticarcinogenic properties. To explore the possibility that piperine has cancer chemopreventive and chemotherapeutic effects in colon cancer, we examined whether piperine inhibits the growth of HT-29 human colon cancer cells and investigated the mechanisms for this effect. Cells were cultured with various concentrations ($0{\sim}40{\mu}M$) of piperine. Piperine decreased the cell viability and induced apoptosis of HT-29 cells. Western blot analysis of total cell lysates revealed that piperine decreases the protein levels of Bcl-2, Mcl-1, and intact Bid but increases Bik levels. Piperine increased the percentage of cells with depolarized mitochondrial membrane, and the release of cytochrome c into cytoplasm. Piperine induced the cleavage of poly (ADP-ribose) polymerase and caspases 8, 9, 7, and 3 and increased the Fas levels. In addition, piperine significantly decreased the protein levels of survivin. The present results indicate that piperine inhibits the growth of HT-29 colon cancer cells by the induction of apoptosis, which may be mediated by its ability to change the Bcl-2 family proteins, increase the activation of caspases, and decrease survivin levels. Overall, our findings suggest that piperine has cancer chemotherapeutic effects in colon cancer.


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