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Reactive Oxygen Species Scavenging Activity of Jeju Native Citrus Peel during Maturation

수확시기별 제주재래종 감귤과피의 활성산소종 소거활성

  • Kim, Yong-Dug (R&D Center, Jeju Special Self-Governing Province Development Corp.) ;
  • Mahinda, Senevirathne (Dept. of Food Bioengineering, Jeju National University) ;
  • Koh, Kyung-Soo (R&D Center, Jeju Special Self-Governing Province Development Corp.) ;
  • Jeon, You-Jin (Faculty of Marine Biomedical Science, Jeju National University) ;
  • Kim, Soo-Hyun (Dept. of Food Bioengineering, Jeju National University)
  • 김용덕 (제주특별자치도개발공사 연구소) ;
  • 마힌다 (제주대학교 식품생명공학과) ;
  • 고경수 (제주특별자치도개발공사 연구소) ;
  • 전유진 (제주대학교 해양의생명과학부) ;
  • 김수현 (제주대학교 식품생명공학과)
  • Published : 2009.04.30

Abstract

This study was conducted to investigate total polyphenolic contents and reactive oxygen species (ROS) scavenging effects of extracts from peels of ten Jeju native citrus fruits according to the harvest from August 2006 to February 2007. Total polyphenolic contents from methanol extracts of citrus peel were the highest in Jigak (Citrus aurantium) and Hongkyool (C. tachibana) by over 200 mg% in the unmatured period, from the late August to the late September, and all the citrus peels mostly decreased while ripening. Scavenging effect of superoxide anion radical showed good correlation with total polyphenolic contents. The unmatured periods of Hongkyool and Jigak were the highest with more than 60%. Hydrogen peroxide scavenging activity was the highest in Sadoogam (C. pseudogulgul) at 73.8% in late August and the second highest activity was observed in Jigak at near 70%, and all the citrus peels decreased during ripening. Hydroxy radical scavenging activity were the highest among all the ROS scavenging activities, especially in the Jigak and Dangyooja (C. grandis) at 75.1% and 74.6%, respectively, and not much affected by increased maturity of the fruits. Nitric oxide radical scavenging activity was the highest in Bungkyool (C. platymama) at 58.4% in late February, and increased with fruit ripening. In this study, Jigak was generally the highest in the polyphenolic contents and ROS scavenging activities, so the further studies are needed for industrial applications.

References

  1. Kwon GJ, Choi DS, Wang MH. 2007. Biological activities of hot water extracts from Euonymus alatus leaf. Korean J Food Sci Technol 39: 569-574
  2. Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J. 2007. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 39: 44-84 https://doi.org/10.1016/j.biocel.2006.07.001
  3. Kim HJ, Jin CB, Lee YS. 2007. Antioxidative activities of phenolic compound isolated from Inonotus obliquus. Kor J Pharmacogn 38: 1-16
  4. Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. 2006. Free radicals, metals and antioxidants in oxidative stressinduced cancer. Chem Biol Interact 160: 1-40 https://doi.org/10.1016/j.cbi.2005.12.009
  5. Masaki H, Skaki S, Atsumi T, Sakurai H. 1995. Activeoxygen scavenging activity of plant extracts. Biol Pharm Bull 18: 162-166 https://doi.org/10.1248/bpb.18.162
  6. Song EY, Choi YH, Kang KH, Koh JS. 1998. Free sugar, organic acid, hesperidin, naringin and inorganic elements changes of Cheju citrus fruits according to harvest date. Korean J Food Sci Technol 30: 306-312
  7. Rhyu MR, Kim EY, Bae IY, Park YK. 2002. Content of naringin, hesperidin and neohesperidin in premature Korean citrus fruits. Korean J Food Sci Technol 34: 132-135
  8. Sohn JS, Kim MK. 1998. Effect of hesperidin and naringin on antioxidative capacity in the rat. Korean J Nutr 31: 687-696
  9. Kawaguchi K, Mizuno T, Aida K, Uchino K. 1997. Hesperidin as an inhibitor of lipases from porcine pancreas and Pseudomonas. Biosci Biotechnol Biochem 61: 102-104 https://doi.org/10.1271/bbb.61.102
  10. Kim YC, Koh KS, Koh JS. 2001. Changes of flavonoids in the peel of Jeju native Citrus fruits during maturation. Food Sci Biotechnol 10: 483-487
  11. Kwon OC, Shin JH, Kang MJ, Lee SJ, Choi SY, Sung NJ. 2006. Antioxidant activity of ethanol extracts from citron (Citrus junos SIEB ex TANAKA) seed. J Korean Soc Food Sci Nutr 35: 294-300 https://doi.org/10.3746/jkfn.2006.35.3.294
  12. Baik JS, Kim SS, Lee JA, Oh TH, Kim JY, Lee NH, Hyun CG. 2008. Chemical composition and biological activities of essential oils extracted from Korean endemic citrus species. J Microbiol Biotechnol 18: 74-79
  13. Choi IW, Choi SY, Nam BR, Kim YS, Choi HD. 2008. Contents of polyphenols and limonoids in citron (Citrus junos Sieb. ex Tanaka) seed extracts and their antioxidant properties. Food Sci Biotechnol 17: 373-378
  14. Kim SS, Baik JS, Oh TH, Yoon WJ, Lee NH, Hyun CG. 2008. Biological activities of Korean Citrus obovoides and Citrus natsudaidai essential oils against acne-inducing bacteria. Biosci Biotechnol Biochem 72: 2507-2513 https://doi.org/10.1271/bbb.70388
  15. AOAC. 1985. Official Method of Analysis. 15th ed. Association of Official Analytical Chemists, Washington, DC, USA. Method 914-915
  16. Nagai T, Inoue I, Inoue H, Suzuki N. 2003. Preparation and antioxidant properties of water extract of propolis. Food Chem 80: 29-33 https://doi.org/10.1016/S0308-8146(02)00231-5
  17. Muller HE. 1995. Detection of hydrogen peroxide produced by microorganism on ABTS peroxidase medium. Zentralbl Bakterio Mikrobio Hyg 259: 151-158
  18. Chung SK, Osawa T, Kawakishi S. 1997. Hydroxyl radical scavenging effects of spices and scavengers from brown mustard (Brassica nigra). Biosci Biotechnol Biochem 61: 118-123 https://doi.org/10.1271/bbb.61.118
  19. Garrat DC. 1964. The quantitative analysis of drugs. Chapmam and Hall Ltd, Tokyo, Japan. Vol 3, p 456-458
  20. Yusof S, Ghazali HM, King GS. 1990. Naringin content in local citrus fruits. Food Chem 37: 113-121 https://doi.org/10.1016/0308-8146(90)90085-I
  21. Herrmann K. 1989. Occurrence and content of hydroxycinnamic and hydroxy-benzoic acid compounds in foods. Crit Rev Food Sci Nutr 28: 315-347 https://doi.org/10.1080/10408398909527504
  22. Park JH, Kang BW, Kim JE, Seo MJ, Lee YC, Lee JH, Joo WH, Choi YH, Lim HS, Jeong YK, Lee BK. 2008. Effect of ethanol extract from peel of Citrus junos and Poncirus trifoliata on antioxidant and immune activity. J Life Sci 18: 403-408 https://doi.org/10.5352/JLS.2008.18.3.403
  23. Anagnostopoulou MA, Kefalas P, Papageorgiou VP, Assimopoulou AN, Boskou D. 2006. Radical scavenging activity of various extracts and fractions of sweet orange peel (Citrus sinensis). Food Chem 94: 19-25 https://doi.org/10.1016/j.foodchem.2004.09.047
  24. Oh HS, An YS, Na IS, Oh MC, Oh CK, Kim SH. 2003. Inhibition of n-nitrosodimethylamine formation of extracts from citrus seeds. Korean J Food Cookery Sci 19: 640-646
  25. Korycka-Dahl M, Richardson T, Hicks CL. 1979. Superoxide dismutase activity in bovine milk serum and its relevance for oxidative stability of milk. J Food Prot 42: 867-871 https://doi.org/10.4315/0362-028X-42.11.867
  26. Kim YC, Chung SK. 2002. Reactive oxygen radical species scavenging effects of Korean medicinal plant leaves. Food Sci Biotechnol 11: 407-411
  27. Shin DB, Lee DW, Yang R, Kim JA. 2006. Antioxidative properties and flavonoids contents of matured Citrus peel extracts. Food Sci Biotechnol 15: 357-362
  28. Chung HY, Kim HB. 2000. In vitro studies on the superoxide scavenging activities, the cytotoxic and the immunomodulating effects of thirteen kinds of herbal extracts. Korean J Food Sci Technol 32: 699-705
  29. Chance B, Sies H, Boveris A. 1979. Hydroperoxide metabolism in mammalian organs. Physiol Rev 59: 527-605
  30. Heo SI, Wang MH. 2008. Antioxidant activity and cytotoxicity effect of extracts from Taraxacum mongolicum H. Kor J Pharmacogn 39: 255-259
  31. Moon YG, Choi KS, Lee KJ, Kim KY, Heo MS. 2006. Screening of antioxidative and antibacterial activity from hot water extracts of indigenous plants, Jeju Island. Korean J Biotechnol Bioeng 21: 164-169
  32. Manian R, Anusuya N, Siddhuraju P, Manian S. 2008. The antioxidant activity and free radical scavenging potential of two different solvent extracts of Camellia sinensis (L.) O. Kuntz, Ficus bengalensis L. and Ficus racemosa L. Food Chem 107: 1000-1007 https://doi.org/10.1016/j.foodchem.2007.09.008
  33. Ding AH, Nathan CF, Stuehr DJ. 1988. Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages. Comparison of activating cytokines and evidence for independent production. J Immunol 141: 2407-2412
  34. Liang YC, Huang YT, Tsai SH, Lin-Shiau SY, Chen CF, Lin JK. 1999. Suppression of inducible cyclooxygenase and inducible nitric oxide synthase by apigenin and related flavonoids in mouse macrophages. Carcinogenesis 20: 1945-1952 https://doi.org/10.1093/carcin/20.10.1945
  35. Choi SY, Ko HC, Ko SY, Hwang JH, Park JG, Kang SH, Han SH, Yun SH, Kim SJ. 2007. Correlation between flavonoid content and the NO production inhibitory activity of peel extracts from various citrus fruits. Biol Pharm Bull 30: 772-778 https://doi.org/10.1248/bpb.30.772

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