Physicochemical Characteristics and Antioxidant Activity of Heated Radish (Raphanus sativus L.) Extracts

열처리 무 추출물의 이화학적 특성과 항산화 활성

  • Lee, Sang-Hoon (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Hwang, In-Guk (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Lee, Youn-Ri (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Joung, Eun-Mi (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Jeong, Heon-Sang (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Lee, Hee-Bong (Dept. of Food Science and Technology, Chungbuk National University)
  • Published : 2009.04.30


This study investigated the changes of physicochemical characteristics and antioxidant activity of heated radish (Raphanus sativus L.). Raw radish was heated at various temperatures ($110{\sim}150^{\circ}C$) for 2 hr. The heated radish was extracted with 70% ethanol and then fractionated with hexane, ethyl acetate, and aqueous. Total polyphenol contents, $IC_{50}$ value of electron donating ability (EDA), and total antioxidant activity (AEAC) increased with increasing heating temperature. The maximum total polyphenol content was $256.26{\pm}9.61$ mg/100 g at $150^{\circ}C$ (control: $27.90{\pm}1.28$ mg/100 g), $IC_{50}$ value was $1.34{\pm}0.004$ mg/mL at $150^{\circ}C$ (control: $34.93{\pm}0.039$ mg/mL), and AEAC was $53.10{\pm}1.155$ mg AA eq/g at $150^{\circ}C$ (control: $6.721{\pm}0.122$ mg AA eq/g). The ethyl acetate fraction showed higher total polyphenol contents and stronger antioxidant activities than hexane and aqueous fractions. Total polyphenol content was 133.62 mg/100 g (at $140^{\circ}C$), $IC_{50}$ values of EDA and AEAC content were 0.39 mg/mL (at $150^{\circ}C$) and 183.72 mg AA eq/g (at $140^{\circ}C$), respectively.


  1. Park YK. 1995. Source and processing technology of vegetable juices and the trend of study. Bull Food Technol 8: 59-68
  2. Jung DH. 1998. Biochemical activity of food. Seonjinmunwhasa, Seoul. p 72-74
  3. Ryu BH. 1999. Antioxidative activity of flavonoids toward modification of human low density lipoprotein. Korean J Food Nutr 12: 320-327
  4. Son JY, Son HS, Cho WD. 1998. Antioxidant effect of onion skin extract. Korean J Soc Food Sci 14: 16-20
  5. Kang JA, Kang JS. 1997. Effect of garlic and orion on plasma and liver cholesterol and triacylglycerol and platelet aggregation in rats fed basal cholesterol supplemented diets. Korean J Nutr 30: 132-138
  6. Rim AR, Jung ES, Kim SY, Lee SC. 2005. Effect of far-infrared irradiation and heat treatment on the antioxidant activity of extracts from defatted soybean meal. Korean J Soc Appl Biol Chem 48: 400-403
  7. Lee YR, Lee YK, Hwang IK, Woo KS, Han CS, Jeong HS. 2008. Evaluation of heated processing temperature and time on functional properties of garlic juice. J Food Sci Nutr 13: 327-333
  8. Woo KS, Jang KI, Kim KY, Lee HB, Jeong HS. 2006. Antioxidative activity of heat treated licorice (Glycyrrhiz uralensis Fisch) extracts. Korean J Food Sci Technol 38: 355-360
  9. Yang SJ, Woo KS, Yoo JS, Kang TS, Noh YH, Lee JS, Jeong HS. 2006. Change of Korean ginseng components with high temperature and pressure treatment. Korean J Food Sci Technol 38: 521-525
  10. Hwang IG, Woo KS, Kim TM, Kim DJ, Yang MH, Jeong HS. 2006. Change of physicochemical characteristics of Korean pear (Pyrus pyrifolia Nskai) juice with heat treatment condition. Korean J Food Sci Technol 38: 342-347
  11. Kwon OC, Woo KS, Kim TM, Kim DJ, Hong JT, Jeong HS. 2006. Physicochemical characteristics of garlic (Allium sativum L.) on the high temperature and pressure treatment. Korean J Food Sci Technol 38: 331-336
  12. Kim HY, Woo KS, Hwang IG, Lee YR, Jeong HS. 2008. Effects of heat treatments on the antioxidant activities of fruits and vegetables. Korean J Food Sci Technol 40: 166-170
  13. Jung MS, Lee GS, Chae HJ. 2004. In vivo biological activity assay of ethanol extract of radish. J Korean Soc Appl Biol Chem 47: 67-71
  14. Yim HB, Lee GS, Chae HJ. 2004. Cytotoxicity of ethanol extract of Raphanus sativus on a human lung cancer cell line. J Korean Soc Food Sci Nutr 33: 287-290
  15. Kang BK, Jung ST, Kim SJ. 2002. Effects of vegetable extracts by solvent separation on alcohol dehydrogenase activity from Saccaromyces cerevisiae. Korean J Food Sci Technol 34: 244-248
  16. Ryu KD, Chung DH, Kim JK. 2000. Comparison of radish cultivars for physicochemical properties and Kakdugi preparation. Korean J Food Sci Technol 32: 681-690
  17. Dewanto V, Xianzhong W, Liu RH. 2002. Processed sweet corn has higher antioxidant activity. J Agric Food Chem 50: 4959-4964
  18. Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1203
  19. Lee JW, Lee SK, Do JH, Shim KH. 1998. Characteristics of the water soluble browning reaction of Korean red ginseng as affected by heating treatment. J Ginseng Res 22: 193-199
  20. Hong HD, Kim YC, Rho JH, Kim KT, Lee YC. 2007. Changes on physicochemical properties of Panax ginseng C.A. Meyer during repeated steaming process. J Ginseng Res 31: 222-229
  21. Park CK, Jeon BS, Kim SC, Chang JK, Lee JT, Yang JW, Shim KH. 2003. Changes of chemical compositions in chicory roots by different roasting processes. Korean J Med Crop Sci 11: 179-185
  22. Lee GC, Ahn SC. 2001. Inhibition effects of caramelization products from sugar solutions subjected to different temperature on polyphenol oxidase. J Korean Soc Food Sci Nutr 30: 1041-1046
  23. Yoon SR, Lee MH, Park JH, Lee IS, Kwon JH, Lee GD. 2005. Changes in physicochemical compounds with heating treatment of ginseng. J Korean Soc Food Nutr 34: 1572-1578
  24. Dewanto V, Wu X, Adom KK, Liu RH. 2002. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J Agric Food Chem 50: 3010-3014
  25. Choi Y, Lee SM, Chun J, Lee HB, Lee J. 2006. Influence of heat treatment on the antioxidant activities and polyphenolic compounds of Shiitake mushroom. Food Chem 99: 381-387
  26. Turkmen N, Sari F, Velioglu YS. 2005. The effects of cooking methods total phenolic and antioxidant activity of selected green vegetables. Food Chem 93: 713-718
  27. Kim SJ, Lee HJ, Park KH, Rhee CO, Lim IJ, Chung HJ, Moon JH. 2008. Isolation and identification of low molecular phenolic antioxidants from ethylacetate layer of Korean black raspberry wine. Korean J Food Sci Technol 40: 129-134
  28. Baek HS, Na YS, Ryu BH, Song SK. 2003. Antioxidant activities of Stachys sieboldii MIQ. stalks. Korean J Biotechnol Bioeng 18: 266-271
  29. Sohn HY, Ryo HY, Jang YJ, Jang HS, Park YM, Kim SY. 2008. Evaluation of antimicrobial, antithrombin, and antioxidant activity of aerial part of Saxifraga stolonifera. Korean J Microbiol Biotechnol 36: 195-200
  30. Myung JE, Hwang IK. 2008. Functional components and antioxidative activities of soybean extracts. Korean Soybean Digest 25: 23-29
  31. Kim SD, Do JH, Oh HI. 1981. Antioxidant activity of Panax ginseng browning products. J Korean Agric Chem Soc 24: 161-166
  32. Manzocco L, Calligaris S, Mastrocola D, Nicoli MC, Lerici CR. 2000. Review of non-enzymatic browning and antioxidant capacity in processed foods. Trends Food Sci Technol 11: 340-346
  33. Nicoli MC, Anese M, Parpinel M. 1999. Influence of processing on the antioxidant properties of fruit and vegetables. Trends Food Sci Technol 10: 94-100

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