Effects of Ore Minerals on the Damages of Animal Cells

광물성 미네랄이 동물세포의 손상에 미치는 효과

Jeon, Yu-Mi;Kim, Jum-Ji;Lee, Mi-Young

  • Published : 2009.12.31


In this study, we investigated the suppressive effects of ore minerals on the allergic cell damages and oxidative cell damages. The ore minerals significantly reduced the productions of tumor necrosis factor-alpha (TNF-${\alpha}$) and interleukin-4 (IL-4) in rat basophilic leukemia cells challenged with 2,4-dinitrophenol-bovine serum albumin (DNP-BSA). Lipoxygenase activity was also reduced by the ore minerals. Moreover, the ore minerals showed weak protective effects on the oxidative damage induced by hydrogen peroxide in pig kidney cells and retinal ganglion cells. Photohemolysis of erythrocytes in the presence of rose-bengal as a sensitizer was also inhibited by ore minerals. These results suggest that the ore minerals may be useful as the protectant for allergic and oxidative cell damages.


Ore Minerals;Allergic cell damage;Oxidative cell damage


  1. Yi J. M., M. S. Kim, S. W. Seo, K. N. Lee, C. S. Yook and H. M. Kim, 2001, Acanthopanax senticosus root inhibits mast cell-dependent anaphylaxis, Clin. Chim. Acta., 312, 163-168
  2. Wuthrich B., 1989, Epidemiology of the allergic diseases: are they really on the increase?, Int. Arch. Allergy Appl. Immunol., 90, 3-10
  3. El Gazzar M., R. El Mezayen, M. R. Nicolls, J. C. Marecki and S. C. Dreskin, 2006, Downregulation of leukotriene biosynthesis by thymoquinone attenuates airway inflammation in a mouse model of allergic asthma, Biochim. Biophys. Acta, 1760, 1088-1095
  4. Zimmermann N., G. K. Hershey, P. S. Foster and M. E. Rothenberg, 2003, Chemokines in asthma: cooperative interaction between chemokines and IL-13, J. Allergy Clin. Immunol., 111, 227-242
  5. Berkman N., V. L. Krishnan, T. Gilbey, R. Newton, B. O'Connor, P. J. Barnes and K. F. Chung, 1996, Expression of RANTES mRNA and protein in airways of patients with mild asthma, Am. J. Respir. Crit. Care Med., 154, 1804-1811
  6. Averbeck M., C. Gebhardt, F. Emmrich, R. Treudler and J. C. Simon, 2007, Immunologic principles of allergic disease, J. Dtsch. Dermatol. Ges., 5, 1015-1028
  7. Park M., I. Y. Kang, S. Y. Rhee, L. I. Jeong, S. Y. Na, K. H. Jeong, S. H. Lee, T. W. Lee and C. G. Ihm, 2006, Polymorphism of IL-1$\beta$, IL-Ra, TNF-$\alpha$ cytokine genes in korean patients with glomerulonephritis, Korean J. Nephrol., 25, 221-228
  8. Jahnsen F. L., P. Brandtzaeg, R. Haye and G. Haraldsen, 1997, Expression of functional VCAM-1 by cultured nasal polyp-derived microvascular endothelium, Am. J. Pathol., 150, 2113-2123
  9. Christodoulopoulos P., L. Cameron, S. Durham and Q. Hamid, 2000, Molecular pathology of allergic disese Ⅱ: upper airway disease, J. Allergy Clin. Immunol., 105, 211-223
  10. Bortuzzo C., R. Hanif, K. Kashfi, L. Staiano-Coico, S. J. Shiff and B. Rigas, 1996, The effect of leukotrienes B and selected HETEs on the proliferation of colon cancer cells, Biochim. Biophys. Acta, 1300, 240-246
  11. Moody T. W., J. Leyton, A. Martinez, S. Hong, A. Malkinson and J. L. Mulshine, 1998, Lipoxygenase inhibitors prevent lung carcinogenesis and inhibit non-small cell lung cancer growth, Exp. Lung Res., 24, 617-628
  12. Sies H., 1991, Oxidative stress: from basic research to clinical application, Am. J. Med., 91, 31S-38S
  13. Vuillaume M., 1987, Reduced oxygen species, mutation, induction and cancer initiation, Mutat. Res., 186, 43-72
  14. Finkel T. and N. J. Holbrook, 2000, Oxidants, oxidative stress and the biology of ageing, Nature, 408, 239-247
  15. Thompson C. B., 1995, Apoptosis in the pathogenesis and treatment of disease, Science, 267, 1456-1462
  16. Goldshmit Y., S. Erlich and R. Pinkas-Karamarski, 2001, Neuregulin rescues PC12-ErbB4 cells from cell death induced by $\H_2O_2$, J. Biol. Chem., 276, 46379-46385
  17. Kitamura Y., T. Ota, Y. Matsuoka, I. Tooyama, H. Kimura, S. Shimohama, Y. Nomura, P. J. Gebicke-Haerter and T. Taniguchi, 1999, Hydrogen peroxide induced apoptosis mediated by p53 protein in glial cells, Glia, 25, 154-164<154::AID-GLIA6>3.0.CO;2-S
  18. Hazen S. L., R. Zhang, Z. Shen, W. Wu, E. A. Podrez, J. C. MacPherson, D. Schmitt, S. N. Mitra, C. Mukhopadhyay, Y. Chen, P. A. Cohen, H. F. Hoff and H. M. Abu-Soud, 1999, Formation of nitric oxide-derived oxidants by myeloperoxidase in monocytes: pathways for monocyte-mediated protein nitration and lipid peroxidation in vivo, Circ. Res., 85, 950-958
  19. Lipton S. A., Y. B. Choi, Z. H. Pan, S. Z. Lei, H. S. Chen, N. J. Sucher, J. Loscalzo, D. J. Singel and J. S. Stamler, 1993, A redox-based mechanism for the neuroprotective and neurodestructive effects of nitric oxide and related nitroso-compounds, Nature, 364, 626-632
  20. Benhar M. and J. S. Stamler, 2005, A central role for S-nitrosylation in apoptosis, Nat. Cell Biol., 7, 645-646
  21. Gomes C. S. F. and J. B. P. Silva, 2007, Minerals and clay minerals in medical geology, Appl. Clay Sci., 36, 4-21
  22. Choi K. M., C. W. Lee and M. Y. Lee, 2008, Effects of ore minerals on the healing of full-thickness skin injury model of rat, J. Environ. Sci., 17, 809-816
  23. Viseras C., C. Aguzzi, P. Cerezo and A. Lopez-Galindo, 2007, Uses of clay minerals in semisolid health care and therapeutic products, Appl. Clay Sci., 36, 37-50
  24. Buseck P. R., D. J. Jacob, M. Posfai, J. Li and J. R. Anderson, 2000, Minerals in the air: an environmental perspective, Int. Geol. Rev., 42, 577-593
  25. Jeong H. J., S. H. Hong, D. J. Lee, J. H. Park, K. S. Kim and H. M. Kim, 2002, Role of Ca(2+) on TNF-alpha and IL-6 secretion from RBL-2H3 mast cells, Cell. Signal., 14, 633-639
  26. Bradding P., I. H. Feather, S. Wilson, P. G. Bardin, C. H. Heusser, S. T. Holgate and P. H. Howarth, 1993, Immunolocalization of cytokines in the nasal mucosa of normal and perennial rhinitic subjects. The mast cell as a source of IL-4, IL-5, and IL-6 in human allergic mucosal inflammation, J. Immunol., 151, 3853-3865
  27. Park S. K., C. W. Lee and M. Y. Lee, 2008, Inhibitory effect of ore minerals on the allergic inflammation in mouse, J. Korean Soc. Appl. Biol. Chem., 51, 269-275
  28. Kang D. K. and M. Y. Lee, 2008, Photoprotective effects of minerals from korean indigenous ores on uva-irradiated human dermal fibroblast, Mol. Cell. Toxicol., 4, 150-156
  29. Park S. K., C. W. Lee and M. Y. Lee, 2009, Antibacterial effects of minerals from ores indigenous to Korea, J. Environ. Biol., 30, 151-154