Establishment of Analytical Method for Fenhexamid Residue in Korean Cabbage, Apple, Mandarin and Green Pepper

HPLC를 이용한 배추, 사과, 감귤, 고추 중 살균제 Fenhexamid의 정밀 분석법 확립

  • Lee, Hye-Ri (Department of Agricultural Biotechnology, Seoul National University) ;
  • Riu, Myoung-Joo (Department of Agricultural Biotechnology, Seoul National University) ;
  • Park, Hee-Won (Department of Agricultural Biotechnology, Seoul National University) ;
  • Na, Ye-Rim (Department of Agricultural Biotechnology, Seoul National University) ;
  • Song, Hyuk-Hwan (Department of Agricultural Biotechnology, Seoul National University) ;
  • Keum, Young-Soo (Department of Agricultural Biotechnology, Seoul National University) ;
  • Zhu, Yongzhe (Qingdao Agricultural University) ;
  • Kim, Jeong-Han (Department of Agricultural Biotechnology, Seoul National University)
  • 이혜리 (서울대학교 농생명공학부) ;
  • 류명주 (서울대학교 농생명공학부) ;
  • 박희원 (서울대학교 농생명공학부) ;
  • 나예림 (서울대학교 농생명공학부) ;
  • 송혁환 (서울대학교 농생명공학부) ;
  • 금영수 (서울대학교 농생명공학부) ;
  • ;
  • 김정한 (서울대학교 농생명공학부)
  • Published : 2009.12.31

Abstract

This study was performed to develop a precise single residue analytical method of fungicide fenhexamid in representative crops for general residue analytical method which could be applied to most of crops. Korean cabbage, mandarin, apple and green pepper were selected, macerated, extracted with acetone, concentrated and partitioned with dichloromethane. Then the extracts were concentrated and cleaned-up through Florisil column with ethyl acetate/0.1% acetic acid in hexane [15:85, (v/v)] before concentration and analysis with HPLC. LOQ (Limit of Quantitation) of fenhexamid was 1 ng (S/N>10) and MQL (Method Quantitative Limit) was 0.01 mg/kg. Recoveries were measured at two fortification levels (10 MQL and 50 MQL) on crop samples and ranged from 85.2% to 94.8% (mean recoveries) and coefficients of variation were <10% regardless of sample type.

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