Effect of Bacillus subtilis C4 and B. cereus D8 on Plant Growth of Canola and Controlling Activity Against Soft Rot and Stem Rot

Bacillus subtilis C4와 B. cereus D8에 의한 유채의 생육증대 및 무름병과 균핵병 방제효과

  • Lee, Jae-Eun (Microbial Resources Lab, Department of Agricultural Microbiology, National Academy of Agricultural Science, RDA) ;
  • Lee, Seo-Hyeun (Microbial Resources Lab, Department of Agricultural Microbiology, National Academy of Agricultural Science, RDA) ;
  • Park, Kyung-Soo (Microbial Resources Lab, Department of Agricultural Microbiology, National Academy of Agricultural Science, RDA) ;
  • Park, Jin-Woo (Microbial Resources Lab, Department of Agricultural Microbiology, National Academy of Agricultural Science, RDA) ;
  • Park, Kyung-Seok (Microbial Resources Lab, Department of Agricultural Microbiology, National Academy of Agricultural Science, RDA)
  • 이재은 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 이서현 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 박경수 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 박진우 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 박경석 (농촌진흥청 국립농업과학원 농업미생물과)
  • Published : 2009.12.31


The effect of two plant growth-promoting rhizobacteria (PGPR) on plant growth and systemic protection against soft rot disease and stem rot disease of canola (Brassica napus), caused by Erwinia carotovora and Sclerotinia sclerotiorum was investigated in a laboratory and a greenhouse. Selected PGPR strains C4 and D8 were treated to canola seeds by soaking. Strains C4 and D8 significantly not only increased plant height and root length about 74% and 40.3% and also reduced disease severity of soft rot disease by 80% by C4 and D8 respectively, compared to the control. Especially strain C4 showed antifungal activity against 6 fungal pathogens, S. sclerotiorum, Rhizoctonia solani, Botrytis cinerea, Fusarium oxysporum, Phytophthora capsici and Colletotrichum acutatum. In greenhouse experiment, the seed treatment of both of them increased plant height, leaf width and leaf length of canola plant to 19.5% and 24.9%, 11.3% and 15.3%, and 14.1% and 20.7% by C4 and D8, respectively, and reduced disease severity of S. sclerotiorium. These results indicate that these two PGPR strains can decrease disease severity and increased plant growth under greenhouse condition. Therefore, these two bacteria have a potential in controlling Sclerotinia stem rot of canola. These strains have to investigate under field condition to determine their role of antibiosis, induced systemic resistance and plant growth promotion on canola.


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