Effect of Bacillus subtilis C4 and B. cereus D8 on Plant Growth of Canola and Controlling Activity Against Soft Rot and Stem Rot

Bacillus subtilis C4와 B. cereus D8에 의한 유채의 생육증대 및 무름병과 균핵병 방제효과

  • Lee, Jae-Eun (Microbial Resources Lab, Department of Agricultural Microbiology, National Academy of Agricultural Science, RDA) ;
  • Lee, Seo-Hyeun (Microbial Resources Lab, Department of Agricultural Microbiology, National Academy of Agricultural Science, RDA) ;
  • Park, Kyung-Soo (Microbial Resources Lab, Department of Agricultural Microbiology, National Academy of Agricultural Science, RDA) ;
  • Park, Jin-Woo (Microbial Resources Lab, Department of Agricultural Microbiology, National Academy of Agricultural Science, RDA) ;
  • Park, Kyung-Seok (Microbial Resources Lab, Department of Agricultural Microbiology, National Academy of Agricultural Science, RDA)
  • 이재은 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 이서현 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 박경수 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 박진우 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 박경석 (농촌진흥청 국립농업과학원 농업미생물과)
  • Published : 2009.12.31

Abstract

The effect of two plant growth-promoting rhizobacteria (PGPR) on plant growth and systemic protection against soft rot disease and stem rot disease of canola (Brassica napus), caused by Erwinia carotovora and Sclerotinia sclerotiorum was investigated in a laboratory and a greenhouse. Selected PGPR strains C4 and D8 were treated to canola seeds by soaking. Strains C4 and D8 significantly not only increased plant height and root length about 74% and 40.3% and also reduced disease severity of soft rot disease by 80% by C4 and D8 respectively, compared to the control. Especially strain C4 showed antifungal activity against 6 fungal pathogens, S. sclerotiorum, Rhizoctonia solani, Botrytis cinerea, Fusarium oxysporum, Phytophthora capsici and Colletotrichum acutatum. In greenhouse experiment, the seed treatment of both of them increased plant height, leaf width and leaf length of canola plant to 19.5% and 24.9%, 11.3% and 15.3%, and 14.1% and 20.7% by C4 and D8, respectively, and reduced disease severity of S. sclerotiorium. These results indicate that these two PGPR strains can decrease disease severity and increased plant growth under greenhouse condition. Therefore, these two bacteria have a potential in controlling Sclerotinia stem rot of canola. These strains have to investigate under field condition to determine their role of antibiosis, induced systemic resistance and plant growth promotion on canola.

References

  1. Chun J. (1995) Computer assisted classification and identification of actinomycetes. Ph. D. Thesis, University of Newcatle, Newcatle upon Tyne, UK
  2. Felsenstein J. (1993) PHYLIP (phylogenetic inference package). Version 3.5c. Department of Genetics, University of Washington, Seattle, USA
  3. Glick B.R. (1995) The enhancement of plant growth by freeliving bacteria. Can. J. Microbiol. 41:109-117 https://doi.org/10.1139/m95-015
  4. Joo G.J., Kim Y.M., Lee I.J., Song K.S., and Rhee I.K.. (2004) Growth promotion of red pepper plug seedlings and the production of gibberellins by Bacillus cereus, Bacillus macroides and Bacillus pumilus. Biotechnol. Lett. 26:487-491 https://doi.org/10.1023/B:BILE.0000019555.87121.34
  5. Kloepper J.W., Tuzun S., Kuc J.A. (1992) Proposed definitions related to induced disease resistance. Biocontrol. Sci. Technol. 2:349-51 https://doi.org/10.1080/09583159209355251
  6. Kloepper J.W., Ryu C.M. and Zhang S. (2004) Induced systemic resistance and promotion of plant growth by Bacillus spp. Phytopathology. 94:1259-1266 https://doi.org/10.1094/PHYTO.2004.94.11.1259
  7. Pieterse C.M.J., van Wees S.C.M., Hoffland E., van Pelt J.A. and van Loon L.C. (1996) Systemic resistance in Arabidopsis induced by biocontrol bacteria is independent of salicylic acid and pathogenesis-related gene expression. Plant Cell 8:1225-1237 https://doi.org/10.1105/tpc.8.8.1225
  8. Park K.S., Paul D., Kim Y.K., Nam K.W., Lee Y.K., Choi H.W. and Lee S.Y. (2007) Induce systemic resistance by Bacillus vallismortis EXTN-1 supressed bacterial wilt in tomato caused by Ralstonia solanacearum. P.P.J. 23:22-25
  9. Raupach G.S., Liu L., Murphy J.F., Tuzun S. and Kloepper J.W. (1996) Induced systemic resistance in cucumber and tomato against cucumber mosaic cucumo virus using plant growth-promoting rhizobacteria (PGPR). Plant Dis. 80:891-894 https://doi.org/10.1094/PD-80-0891
  10. Raymer P.L. (2002) Canola: An emerging oilseed crop. Trends in new crops and new uses. ASHS Press, Alexandria, VA
  11. Tu J.C. (1997) Biological control of white mould in white bean using Trichoderma viride, Gliocladium roseum and Bacillus subtillis as protective foliar spray. In proceedings of 49th International Symposium on Crop Protection. 6. May 1997, Genr. Part IV. Meded. Fac. Landbouwkd. Toegep. Biol. Wet. Univ. Gent, 62:979-986
  12. Yu G.Y., Sinclair J.B., Hartman G.L. and Bertagnolli B.L. (2002) Production of iturin A by Bacillus amyloliquefaciens suppressing Rhizoctonia solani. Soil. Biol. Biochem. 34:955-963 https://doi.org/10.1016/S0038-0717(02)00027-5
  13. Asaka O. and Shoda M. (1996) Biocontrol of Rhizoctonia solani damping-off of tomato with Bacillus subtilis RB14. Appl. Environ. Microbiol. 62:4081-4085
  14. Choudhary D.K., Johri B.N. (2009) Interactions of Bacillus spp. and plants – With special reference to induced systemic resistance (ISR). Microbiol. Res. 164:493-513 https://doi.org/10.1016/j.micres.2008.08.007
  15. Godoy G., Steadman J.R., and Yuen G. (1990) Bean blossom bacteria have potential for biological control of white mold disease caused by Sclerotinia sclerotiorum. Annu. Rep. Bean. Improv. Coop. 33:45-46
  16. Pinchuk I.V., Bressollier P., Sorokulova I., Verneuil B. and Urdaci M.C. (2002) Amicoumacin antibiotic production and genetic diversity of Bacillus subtilis strains isolated from different habitats. Res. Microbiol. 153:269-276 https://doi.org/10.1016/S0923-2508(02)01320-7
  17. Garcia, J.A.L., Probanza A., Ramos B., Palomino M.R. and Manero F.J.G., (2004) Effect of inoculation of Bacillus licheniformis on tomato and pepper. Agronomie. 24:169-176 https://doi.org/10.1051/agro:2004020
  18. Idriss E.E, Makarewicz O., Farouk A., Rosner K., Greiner R., Bochow H., Richter T. and Borriss R. (2002) Extracellular phytase activity of Bacillus amyloliquefaciens FZB45 contributes to its plant-growth-promoting effect. Microbiology. 148:2097-2109
  19. Kloepper J.W. and Schroth M.N. (1978) Plant growth-promoting rhizobacteria on radishes. In: Proceedings of 4th International Conference on Plant Pathogenic Bacteria 2:879-882
  20. Wei G., Kloepper J.W. and Tuzun S. (1991) Induction of systemic resistance of cucumber to Colletotrichum orbiculare by select strains of plant growth-promoting rhizobacteria. Phytopathology. 81:1508-1512 https://doi.org/10.1094/Phyto-81-1508
  21. Wei G., Kloepper J.W. and Tuzun S. (1996) Induced systemic reisistance to cucumber disease and increased plant growth by plant growth-promoting rhizobacteria under field conditions. Phytopathology. 86:221-224 https://doi.org/10.1094/Phyto-86-221
  22. Kimura M. (1980) A simple method for estimating evolutionary rate of base substitution through comparative studies of nucleotide sequence. J. Mol. Evol. 16:111-120 https://doi.org/10.1007/BF01731581
  23. Kloepper J.W., Lifshitz R. and Zablotowicz R.M. (1989) Free living bacterial inocula for enhancing crop productivity. Trends. Biotechnol. 7:39-44 https://doi.org/10.1016/0167-7799(89)90057-7
  24. Boyetchko S.M. (1999) Biological control agents of canola and rapeseed diseases-status and practical approaches. In Mukerji K., Chamola B. and Upadhyay R., eds. Biotechmological approaches in biocontrol of plant pathogens. New York: Kluwer Acadimic / Plemum Publishers. pp. 51-71
  25. Chen T.W. and Wu W.S. (1999) Biological control of carrot black rot. J. Phytopathol. 147:99-104
  26. van Peer R., Niemann G.J. and Schippers B. (1991) Induced resistance and phytoalexin accumulation in biological control of fusarium wilt of carnation by Pseudomonas sp. strain WCS417r. Phytopathology. 81:728-734 https://doi.org/10.1094/Phyto-81-728
  27. Raupach G.S. and Kloepper J.W. (1998) Mixtures of plant growth-promoting rhizobacteria enhance biological control of multiple cucumber pathogens. Phytopathology. 88:1158-1164 https://doi.org/10.1094/PHYTO.1998.88.11.1158
  28. Jetiyanon K. and Kloepper J.W. (2002) Mixtures of plant growth-promoting rhizobacteria for induction of systemic resistance against multiple plant disease. Biol. Control. 24:285-291 https://doi.org/10.1016/S1049-9644(02)00022-1
  29. Ryu C.-M., Farag M.A., Hu C-H., Reddy M.S., Wei H-X., Par P.W. and Kloepper J.W. (2003) Bacterial volatiles promote growth in Arabidopsis. Proc. Natl. Acad. Sci. 100:4927-4932 https://doi.org/10.1073/pnas.0730845100
  30. Glick B.R., Patten C.L., Holguin G. and Penrose D.M. (1999). Biochemical and genetic mechanisms used by plant growth promoting bacteria. Imperial College Press, London, United Kingdom, pp. 267
  31. Hoffland E., Hakulinen J. and van Pelt J.A. (1996) Comparison of systemic resistance induced by avirulent and nonpathogenic Pseudomonas species. Phytopathology. 86:757-762 https://doi.org/10.1094/Phyto-86-757
  32. Kloepper J.W. (1993) Plant growth-promoting rhizobacteria as biological control agents. P. 255-274 in Soil microbial ecology-applications in agricultural and environmental management. Metting, F.B., Jr. (ed.). Marcel Dekker, New York
  33. Fuller P., Coyne D. and Steadman J. (1984) Inheritance of resistance to white mold disease in a diallel cross of dry beans. Crop. Sci. 24:929-933 https://doi.org/10.2135/cropsci1984.0011183X002400050025x
  34. Kloepper J.W. (1994) Plant growth-promoting rhizobacteria. In: Okon, Y. (Ed.), Azospirillum/Plant Associations. CRC Press, Boca Raton, FL, pp. 137-166
  35. Podile A.R. and Prakash A.P. (1996) Lysis and biological control of Aspergillus niger by Bacillus subtilis AF1. Can. J. Microbiol. 42:533-538 https://doi.org/10.1139/m96-072
  36. Priest F. (1993) Systematics and ecology of Bacillus. In: Bacillus subtilis and other gram-positive bacteria, biochemistry, physiology, and molecular genetics. Washington:ASM Press; pp. 3-16
  37. Fernando W.G.D., Nakkeeran S., Zhang Y. and Savchuk S. (2007) Biological control of Sclerotinia sclerotiorum (Lib.) de Bary by Pseudomonas and Bacillus species on canola petals. Crop. Protect. 26:100-107 https://doi.org/10.1016/j.cropro.2006.04.007
  38. Sorensen J. (1997) The rhizosphere as a habitat for soil microorganisms. In van Elsas J. D., Trevors J. T. and Welington E. M. H. (Eds.). Modern Soil Ecology (pp. 21-46). New York: Marcel Dekker, Inc.
  39. Chanway C.P. (1997) Inoculation of tree roots with plant growth-promoting soil bacteria: An emerging technology for reforestation. For. Sci. 43:99-112
  40. Park M.S., Jung S.K., Lee M.S., Kim K.O., Do J.O., Lee K.H., Kim S.B. and Bae K.S. (2005) Isolation and characterization of bacteria associated with two sand dune plant species Calystegia soldanella and Elymus mollis. J. Microbiol. 43:219-227
  41. Liu L., Kloepper J.W. and Tuzum S. (1995) Induction of systemic resistance in cucumber by plant growth-promoting rhizobacteria: Duration of protection and effect of host resistance on protection and root colonization. Phytopathology. 85: 1064-1068 https://doi.org/10.1094/Phyto-85-1064
  42. Lucas-Garcia J.A., Probanza A., Ramos B., Colon-Flores J.J. and Gutierez-Manero F.J. (2004) Effects of plant growth promoting rhizobacteria (PGPRs) on the biological nitrogen fixation, nodulation and growth of Lupinus albus I.cv. Multolupa, Eng. Life Sci. 4:71-77 https://doi.org/10.1002/elsc.200400013
  43. van Loon L.C., Bakker P.A.H.M and Pieterse C.M.J. (1998) Systemic resistance induced by rhizosphere bacteria. Annu. Rev. Phytopathol. 36:453-483 https://doi.org/10.1146/annurev.phyto.36.1.453