Characterization of the Acetolactate synthase (ALS) gene and Molecular Assay of Mutations Associated with Sulfonylurea Herbicide Resistance of Monochoria vaginalis

물달개비의 Acetolactate synthase (ALS) 유전자의 특성과 Sulfonylurea 제초제 저항성과 관련 돌연변의 분자생물학적 접근

  • 박태선 (국립식량과학원 벼맥류부 벼육종재배과) ;
  • 박홍규 (국립식량과학원 벼맥류부 벼육종재배과) ;
  • 구본일 (국립식량과학원 벼맥류부 벼육종재배과) ;
  • 김영두 (국립식량과학원 벼맥류부 벼육종재배과) ;
  • 고재권 (국립식량과학원 벼맥류부 벼육종재배과) ;
  • 이인용 (국립농업과학기술원 농약안정성부 농약평가과) ;
  • 박재읍 (국립농업과학기술원 농약안정성부 농약평가과)
  • Published : 2009.12.31


This research aims to contribute the characterization of acetolactate synthase (Ec; ALS) and the resistance mechanism by sequence analysis of ALS gene of the sulfonylurea-resistant and -susceptible Monochoria vaginalis. The ALS gene was obtained from susceptible (S) and resistant (R) M. vaginalis to sulfonylurea herbicides (SUs). The 815 bp the fragment and the genomic DNA sequence coding for acetolactate synthase (ALS) of S and R biotypes of M. vaginalis were cloned and sequenced. Nineteen clones were divided greatly into 4 groups as result of sequencing. The first group was not difference to S type, the second group was amino acid of P197S which found point mutations causing substitution of serine for proline at amino acid 197, the third group was observed greatly other part of 6 places than group 1, and the fourth group appeared the intergrade of group 1 and 3. Therefore, it could be assumed what ALS gene of various types can be one plant. The peptide of the 13 amino acid Domain A region for ALS genes from R biotype of M. vaginalis differed from that of the S biotype by one base substitution at proline codon of Domain A. It could also be confirmed that point mutation of serine for proline at amino acid 197.


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