Insecticidal activity of Diamondback Moth, Plutella xylostella against Bacillus thuringiensis and Neem oil

Bacillus thuringiensis와 Neem oil에 대한 배추좀나방의 살충활성

  • Cho, Min-Su (Pesticide Safety Evaluation Division, National Academy of Agricultural Science, Rural Development Adminstration) ;
  • Choi, Su-Yeon (Dept. Applied Biology, College of Agriculture and Life Science, Chungnam National University) ;
  • Kim, Tae-Whan (Dept. Applied Biology, College of Agriculture and Life Science, Chungnam National University) ;
  • Park, Chan (Dept. Applied Biology, College of Agriculture and Life Science, Chungnam National University) ;
  • Kim, Dam-A (Pesticide Safety Evaluation Division, National Academy of Agricultural Science, Rural Development Adminstration) ;
  • Kim, Young-Rim (Pesticide Safety Evaluation Division, National Academy of Agricultural Science, Rural Development Adminstration) ;
  • Oh, Se-Mun (Pesticide Safety Evaluation Division, National Academy of Agricultural Science, Rural Development Adminstration) ;
  • Kim, Sung-Woo (Pesticide Safety Evaluation Division, National Academy of Agricultural Science, Rural Development Adminstration) ;
  • Youn, Young-Nam (Dept. Applied Biology, College of Agriculture and Life Science, Chungnam National University) ;
  • Yu, Yong-Man (Dept. Applied Biology, College of Agriculture and Life Science, Chungnam National University)
  • 조민수 (농촌진흥청 국립농업과학원 농약평가과) ;
  • 최수연 (충남대학교 농업생명과학대학 응용생물과) ;
  • 김태환 (충남대학교 농업생명과학대학 응용생물과) ;
  • 박찬 (충남대학교 농업생명과학대학 응용생물과) ;
  • 김담아 (농촌진흥청 국립농업과학원 농약평가과) ;
  • 김영림 (농촌진흥청 국립농업과학원 농약평가과) ;
  • 오세문 (농촌진흥청 국립농업과학원 농약평가과) ;
  • 김성우 (농촌진흥청 국립농업과학원 농약평가과) ;
  • 윤영남 (충남대학교 농업생명과학대학 응용생물과) ;
  • 유용만 (충남대학교 농업생명과학대학 응용생물과)
  • Published : 2009.12.31

Abstract

For the environmental friendly management of diamondback moth, Plutella xylostella (L.), Bacillus thuringiensis subsp. kurstaki ($30{\times}10^8\;cfu/mg$) and neem oil (0.5% azadirachtin) were used as green control agencies with mixed and alternative treatments on the chinese cabbage. When Bacillus thuringiensis subsp. kurstaki was applied to 1st and 2nd larva of P. xylostella with recommended concentration, their mortalities were reached to 100% by 2 days after treatment. In case of azadirachtin, its effect of mortality was continued for 7 days, and reached to 100% mortality. $LC_{50}$ values of Bacillus thuringiensis subsp. kurstaki and azadirachtin against 1st, 2nd, 3rd and 4th larva of P. xylostella were $2.8{\times}10^4$, $3.1{\times}10^4$, $3.4{\times}10^4$ and $1.5{\times}10^5\;cfu/ml$, and 2.7, 3.9, 4.7 and 7.1 ppm, respectively. The number of laid eggs of P. xylostella was reduced to 57.5 at 25 ppm of azadirachtin compared with control treatment. The hatch ratio was not significantly different with Bacillus thuringiensis subsp. kurstaki treatment in comparison with control treatment. However, when azadirachtin was applied, their hatch ratio were reduced to 25.8 and 45.4% at 25 and 50 ppm, respectively. On the other hand, emergence rate of eggs was not different with Bacillus thuringiensis subsp. kurstaki treatment, but 45.4% was shown in azadirachtin treatment with 50 ppm in comparison with control. When the mixture with Bacillus thuringiensis subsp. kurstaki and azadirachtin was applied to adults of P. xylostella, their mortality was higher than Bacillus thuringiensis subsp. kurstaki treatment only. These results are supposed that the mixture of Bacillus thuringiensis subsp. kurstaki and azadirachtin might be used as green control agents for reducing the demage of diamondback moth in the Chinse cabbage.

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